Surgical Endoscopy

, Volume 14, Issue 5, pp 448–451 | Cite as

Hepatic tumor spread of colorectal cancer in a laparoscopic animal model

  • C. N. Gutt
  • Z. G. Kim
  • T. Gessmann
  • M. Lorenz
  • V. Paolucci
Article

Abstract

Background: We devised a standardized animal model to study the impact of laparoscopic colorectal surgery on intrahepatic tumor cell growth.

Methods: The technique of laparoscopic surgery in the rat was extended by endoscopic inoculation of colon cancer cells (CC531) into the portal vein (1 × 104, 5 × 104, 1 × 105, 3 × 105, 5 × 105 cells/ml) of WAG/Rij rats (n= 25). As controls, five animals underwent laparotomy and open intraportal inoculation of 5 × 104 cells/ml.

Results: Hepatic tumor growth occurred after inoculation of 5 × 104, 1 × 105, 3 × 105, and 5 × 105 cells/ml. Extrahepatic tumor and conflating hepatic tumor was observed after the inoculation of 1 × 105, 3 × 105, and 5 × 105 cells/ml. Concentrations of 5 × 104 cells/ml injected either laparoscopically or via an open technique led to single hepatic tumor nodules. No tumor growth was seen after inoculation of 1 × 104 cells/ml.

Conclusions: Laparoscopic intraportal tumor cell inoculation is a feasible technique to create hepatic metastases. The inoculation of 5 × 104 CC531 cells leads to reliable cell growth that can be used to investigate the impact of various laparoscopic techniques on tumor spread.

Key words: Laparoscopy — Colorectal surgery — Intrahepatic tumor cells — Hepatic metastases — Cancer — Animal model — Liver 

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Copyright information

© Springer-Verlag New York Inc. 2000

Authors and Affiliations

  • C. N. Gutt
    • 1
  • Z. G. Kim
    • 1
  • T. Gessmann
    • 1
  • M. Lorenz
    • 1
  • V. Paolucci
    • 1
  1. 1.Department of General and Vascular Surgery, Johann Wolfgang Goethe University, Theodor-Stern-Kai 7, 60590 Frankfurt/Main, GermanyDE

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