Surgical Endoscopy

, Volume 28, Issue 8, pp 2437–2442 | Cite as

Gut inflammation and expression of ICC in a fetal lamb model of fetoscopic intervention for gastroschisis

  • Thomas Krebs
  • Michael Boettcher
  • Hansjörg Schäfer
  • Georg Eschenburg
  • Katharina Wenke
  • Birgit Appl
  • Beate Roth
  • Thomas Andreas
  • Carla Schmitz
  • Rebecca Fahje
  • Birthe Jacobsen
  • Bastian Tiemann
  • Konrad Reinshagen
  • Kurt Hecher
  • Robert Bergholz
Article

Abstract

Background

The pathogenesis of intestinal dysmotility in gastroschisis is not completely understood. Peel formation and disorganization of interstitial Cajal cells (ICC) have been proposed in humans. The aim of this study was to evaluate the impact of prenatal coverage of gastroschisis on gut inflammation and expression of ICC in a fetal lamb model.

Methods

Twenty-one German blackhead sheep with an abdominal wall defect that was created fetoscopically on day 77 of 145 days gestation were used in this study. Intrauterine surgery with the aim to cover the defect was performed 3 weeks later; two fetuses were covered completely, 5 partially and 11 remained uncovered. Three fetuses without gastroschisis were used as controls. All fetuses were retrieved by cesarean section at day 135. Samples of the small intestine were stained with hematoxylin and eosin for histologic analysis of peel formation and serosal and muscular thickness. For ICC detection, immunohistochemistry using anti-CD117 (c-Kit) antibody was used.

Results

In all samples with exposure to amniotic fluid, peel formation and significantly decreased ICC were found. Complete coverage reduced peel formation and disorganization of ICC compared to uncovered animals almost to the level of controls.

Conclusions

Peel formation and ICC derangement were significantly reduced by prenatal coverage of gastroschisis. Moreover, this animal model mimics the histopathological bowel changes as seen in human gastroschisis and may, therefore, be used for further research on the pathophysiology and fetal therapy of this malformation.

Keywords

Fetoscopy Fetal surgery Gastroschisis Motility ICC Pathology Animal model 

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Thomas Krebs
    • 1
  • Michael Boettcher
    • 1
  • Hansjörg Schäfer
    • 2
  • Georg Eschenburg
    • 1
  • Katharina Wenke
    • 1
  • Birgit Appl
    • 1
  • Beate Roth
    • 1
  • Thomas Andreas
    • 3
  • Carla Schmitz
    • 1
  • Rebecca Fahje
    • 1
  • Birthe Jacobsen
    • 1
  • Bastian Tiemann
    • 4
  • Konrad Reinshagen
    • 1
  • Kurt Hecher
    • 3
  • Robert Bergholz
    • 1
  1. 1.Department of Pediatric SurgeryUniversity Medical Center Hamburg-EppendorfHamburgGermany
  2. 2.Department of PathologyUniversity Medical Center Hamburg-EppendorfHamburgGermany
  3. 3.Department of Obstetrics and Fetal MedicineUniversity Medical Center Hamburg-EppendorfHamburgGermany
  4. 4.Department of Experimental Animal ResearchUniversity Medical Center Hamburg-EppendorfHamburgGermany

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