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Videoscopic fluorescence diagnosis of peritoneal and thoracic metastases from human hepatoblastoma in nude rats

  • H. Till
  • F. BergmannEmail author
  • R. Metzger
  • B. Haeberle
  • K. Schaeffer
  • D. von  Schweinitz
  • R. L. Prosst
Article

Abstract

Background

Various medical disciplines are employing photodynamic diagnosis (PDD) when searching for malignancies. It is still unknown whether pediatric solid tumors such as hepatoblastoma are susceptible to this technique as well.

Methods

Human hepatoblastoma cells were injected into the abdomen or right thoracic cavity of nude rats. Tumor growth was allowed for 7 weeks. Then, photosensitization was induced by peritoneal lavage with 5-aminolevulinic acid (ALA). Applying the Storz PDD system and one 4-mm scope, all animals were investigated by videoscopic white light diagnosis (WD) and PDD. Suspicious lesions were marked and analyzed by spectrometry and histology.

Results

Positive fluorescence was documented for every tumor seen by WD in the abdomen or right thoracic cavity. Spectrometry of lesions showed a 6.34-fold increased fluorescence intensity. Histology revealed hepatoblastoma in all specimens.

Conclusions

Human hepatoblastoma can be detected by PDD in a rat model. Considering the clinical success of this method in other specialties, our findings indicate that further investigations to evaluate the benefit of PDD for children with hepatoblastoma should be performed.

Keywords

Fluorescence diagnosis Laparoscopy Thoracoscopy Hepatoblastoma Pediatric oncology 

Notes

Acknowledgments

The authors thank N. Decker for her invaluable help. This work was supported by Karl Storz GmbH (equipment), Medac GmbH (ALA), and Prof. Dr. med. T. Pietsch (HuH 6 cells).

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Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  • H. Till
    • 1
  • F. Bergmann
    • 1
    Email author
  • R. Metzger
    • 1
  • B. Haeberle
    • 1
  • K. Schaeffer
    • 1
  • D. von  Schweinitz
    • 1
  • R. L. Prosst
    • 2
  1. 1.Department of Pediatric SurgeryDr. V. Hauner Children’s Hospital, University of MunichMünchenGermany
  2. 2.Department of SurgeryUniversity Hospital Mannheim, University of HeidelbergGermany

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