Advertisement

Dysphagia

, Volume 27, Issue 2, pp 277–283 | Cite as

Extracellular Matrix Composition of the Cricopharyngeus Muscle

  • Raquel Aguiar TavaresEmail author
  • Luiz Ubirajara Sennes
  • Thais Mauad
  • Rui Imamura
  • Luiz Fernando Ferraz da Silva
  • Ricardo Luis Carrau
Original Article
  • 189 Downloads

Abstract

The aim of this study was to analyze the presence and distribution of total collagen, type I and type III collagen, elastic fibers, fibronectin, and versican in the endomysium of cricopharyngeus muscles from adults of various ages. The study was a cross-sectional analysis of human cricopharyngeus muscles. Twenty-seven muscles obtained from autopsies of men and women ranging in age from 28 to 92 years were analyzed with the Picrosirius method, oxidized Weigert resorcin-fuchsin, immunohistochemistry, and image analysis. Collagen had the highest density among the analyzed components. Elastic fibers surrounded each muscle cell; they were aligned longitudinally by their long axis and associated with traversing fibers, thereby forming a fiber network with embedded muscle cells. The fibronectin and versican contents varied widely among the specimens. We found no statistically significant differences between the proportion of extracellular matrix (ECM) components and factors such as gender and race. We conclude that the higher proportion of type I and type III collagen is compatible with the cricopharyngeus muscle’s sphincteric behavior, and the arrangement of the elastic fibers may also contribute to the muscle’s elasticity. We found no statistically significant correlation between the ECM components and age.

Keywords

Collagen fibers Elastic fibers Fibronectin Versican Cricopharyngeus muscle Immunohistochemistry 

Notes

Acknowledgments

We thank the autopsy assistants of the SVOC-USP who helped gather the study material, and we thank Angela Batista Gomes dos Santos and Maria Cristina Rodrigues Medeiros for their support in the immunohistochemical procedures. This work was supported by FAPESP (São Paulo State Research Agency—Brazil) and CNPq (National Council for Scientific and Technological Department—Brazil).

References

  1. 1.
    Goyal RK, Martin SB, Shapiro J, Spechler SJ. The role of the cricopharyngeus muscle in pharyngoesophageal disorders. Dysphagia. 1993;8:252–8.PubMedCrossRefGoogle Scholar
  2. 2.
    Sivarao DV, Goyal RJ. Functional anatomy and physiology of the upper esophageal sphincter. Am J Med. 2000;108(4A):27(S)–37(S).Google Scholar
  3. 3.
    Kristmundsdottir F, Mohon M, Froes MMQ, Cumming WJK. Histomorphometric and histopathological study of the human cricopharyngeus muscle in health and in motor neuron disease. Neuropathol App Neurobiol. 1990;16:461–75.CrossRefGoogle Scholar
  4. 4.
    Bonington A, Mahon M, Whitmore I. A histological and histochemical study of the cricopharyngeus muscle in man. J Anat. 1988;156:27–37.PubMedGoogle Scholar
  5. 5.
    Laurikainen E, Aitasalo K, Halonen P, Falck B, Kalimo H. Muscle pathology in idiopathic cricopharyngeus dysphagia. Enzyme histochemical and electron microscopic findings. Eur Arch Otorhinolaryngol. 1992;249:216–23.PubMedCrossRefGoogle Scholar
  6. 6.
    Kelly JH, Kencl RW. Myology of the pharyngoesophageal segment: gross anatomy and histologic characteristics. Laryngoscope. 1996;106:713–20.PubMedCrossRefGoogle Scholar
  7. 7.
    Cruse JP, Edwards DAW, Smith JF, Wyllie JH. The pathology of cricopharyngeal dysphagia. Histopathology. 1979;3:223–32.PubMedCrossRefGoogle Scholar
  8. 8.
    Browlow H, Whitemore I, Willian PLT. A quantitative study of the histochemical and morphometric characteristics of the human cricopharyngeus muscle. J Anat. 1989;166:67–75.Google Scholar
  9. 9.
    Montes GS. Structural biology of the fibers of the collagenous and elastic systems. Cell Biol Int. 1996;20(1):15–27.PubMedCrossRefGoogle Scholar
  10. 10.
    Bradbury P, Rae K. Connective tissue and stains. In: Bancroft JD, Steves A, editors. Theory and practice of histological techniques. 4th ed. New York: Churchill Livingstone; 1996. p. 113–38.Google Scholar
  11. 11.
    Araújo BB, Dolhnikoff Silva LF, et al. Extracellular matrix components and regulators in the airway smooth muscle in asthma. Eur Res J. 2008;32(1):61–9.CrossRefGoogle Scholar
  12. 12.
    Shaker R, Kern M, Bardan E, et al. Augmentation of deglutitive upper esophageal sphincter opening in the elderly by exercise. Am J Physiol. 1997;272:1518–22.Google Scholar
  13. 13.
    Järvinen TA, Józsa L, Kannus P, et al. Organization and distribution of intramuscular connective tissue in normal and immobilized muscles. J Musc Res Cell Motil. 2002;23:245–54.CrossRefGoogle Scholar
  14. 14.
    Kovanen V. Effects of ageing and physical training on rat skeletal muscle. Acta Physiol Scand Suppl. 1989;577:1–56.PubMedGoogle Scholar
  15. 15.
    Light N, Champion AE. Characterization of muscle epimysium, perymisium and endomysium collagens. Biochem J. 1984;219:1017–26.PubMedGoogle Scholar
  16. 16.
    Goyal RJ, Sinvarao DV. Functional anatomy and physiology of swallowing and esophageal motility. In: Castell DO, Richter JE, editors. The esophagus. Philadelphia: Lippincott Williams & Wilkins; 1999. p. 1–31.Google Scholar
  17. 17.
    Ross R. The elastic fiber. J Histochem Cytochem. 1973;21(3):199–208.PubMedCrossRefGoogle Scholar
  18. 18.
    Hynes RO, Yamada KM. Fibronectins: multifunctional modular glycoproteins. J Cell Biol. 1982;95:369–77.PubMedCrossRefGoogle Scholar
  19. 19.
    Gulati AK, Reddi AH, Zlewski AA. Distribution of fibronectin in normal and regenerating skeletal muscle. Anat Rec. 1982;204:175–83.PubMedCrossRefGoogle Scholar
  20. 20.
    Buhler RB, Sennes LU, Mauad T, et al. Collagen fiber and versican distribution with the lamina propria of fetal vocal folds. Laryngoscope. 2007;117:1–4.Google Scholar
  21. 21.
    Fomovsky GM, Thomopouulos S, Holmes JW. Contribution of extracellular matrix to the mechanical properties of the heart. J Mol Cel Cardiol. 2010;48(3):490–6.CrossRefGoogle Scholar
  22. 22.
    Souza RR. Aging of myocardial collagen. Biogerontology. 2002;3:325–35.PubMedCrossRefGoogle Scholar
  23. 23.
    Puff A, Langer H. Das Problem der diastolischen Entfaltung der herzkammer (Eine Untersuchung eber das elastichen Gewebe im Myocard). Gegenbaurs Morphol Jahrb. 1965;107:184.PubMedGoogle Scholar
  24. 24.
    Farahnaz F, Contard F, Corbier A, et al. Fibronectin expression during physiological and pathological cardiac growth. J Mol Cel Cardiol. 1995;27(4):981–90.CrossRefGoogle Scholar
  25. 25.
    Rodrigues CJ, Rodrigues Júnior AJ. A comparative study of aging of the elastic fibers system of the diaphragm and the rectus abdominis muscles in rats. Braz J Med Biol Res. 2000;33(12):1449–54.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Raquel Aguiar Tavares
    • 1
    • 2
    Email author
  • Luiz Ubirajara Sennes
    • 1
  • Thais Mauad
    • 3
  • Rui Imamura
    • 1
  • Luiz Fernando Ferraz da Silva
    • 3
  • Ricardo Luis Carrau
    • 4
  1. 1.Otolaryngology DepartmentUniversity of São Paulo, School of MedicineSão PauloBrazil
  2. 2.FortalezaBrazil
  3. 3.Pathology DepartmentUniversity of São Paulo, School of MedicineSão PauloBrazil
  4. 4.Otolaryngology DepartmentUniversity of Pittsburgh, School of MedicinePittsburghUSA

Personalised recommendations