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Bioprocess and Biosystems Engineering

, Volume 24, Issue 1, pp 25–31 | Cite as

Dye-ligand poly(GMA–TAIC–DVB) affinity adsorbent for protein adsorption

  •  Y.-H. Yu
  •  B. Xue
  •  Y. Sun

Abstract.

Macroporous poly(glycidyl methacrylate–triallyl isocyanurate–divinylbenzene) was prepared by a radical suspension copolymerization. Reaction of the copolymer with 2-hydroxyethyl amine was employed to obtain a hydrophilic matrix. An affinity dye, Cibacron blue 3GA, was then coupled covalently to prepare a novel macroporous affinity adsorbent. The surface and pore structure of the affinity adsorbent were examined by scanning electron micrography (SEM). SEM observations showed that the affinity adsorbent abounded in macropores. Bovine serum albumin (BSA) and lysozyme (Lys) were used as samples to examine the adsorption properties of the adsorbent. Under appropriate conditions, the affinity adsorbent had a capacity of 15.5 mg BSA/g and 22.3 mg Lys/g (wet adsorbent weight). The adsorbed proteins could be desorbed by increasing liquid phase ionic strength or by using a NaOH solution, and the adsorbent could be recycled for protein adsorption.

Cibacron blue 3GA Affinity adsorbent Protein adsorption 

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Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  •  Y.-H. Yu
    • 1
  •  B. Xue
    • 1
  •  Y. Sun
    • 1
  1. 1.Department of Biochemical Engineering, Tianjin University, Tianjin 300072, P.R. China

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