Bioprocess Engineering

, Volume 16, Issue 6, pp 345–347

Effect of shear rate on hybridoma cell metabolism

  • N. Shiragami

Abstract

The effect of shear rate on cell growth and monoclonal antibody production of hybridoma cells was studied. The dependence of agitation rate on antibody production is discussed by measuring the amount of monoclonal antibody in cells cultured by a spinner vessel. The effect of shear rate is also studied by exposing a homogeneous shear flow to hybridoma cells in a cone-and-plate viscometer. The dependence of shear rate on hybridoma cells was observed and the increase of antibody production was arised from the increase of secretion from cells.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • N. Shiragami
    • 1
  1. 1.Department of Biological and Chemical Engineering, Gunma University, 1-5-1 Tenjin-cho, Kiryu, Gunma, JapanJP

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