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Bioprocess Engineering

, Volume 16, Issue 5, pp 261–264 | Cite as

Electroporation: basic principles, practical considerations and applications in molecular biology

  • G. Lakshmi Prasanna
  • T. Panda

Abstract

There are many approaches available for introducing foreign DNA into eukaryotes and prokaryotes. The most commonly used technique and currently of more practical interest is the electroporation. This review will focus on the electroporation as a promising, highly efficient and effective means of gene transfer. We summarize a detailed assessment of the various parameters and conditions that govern electroporation of a wide range of cell types.

Keywords

Molecular Biology Gene Transfer Basic Principle Practical Interest Effective Means 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • G. Lakshmi Prasanna
    • 1
  • T. Panda
    • 1
  1. 1.Biochemical Engineering Division, Department of Chemical Engineering, Indian Institute of Technology, Madras 600 036, IndiaIN

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