Response of extracellular polymeric substances and enzymatic activity to salinity for the waste activated sludge anaerobic fermentation process

  • Baodan JinEmail author
  • Jintao Niu
  • Ju Zhang
  • Jiahui Niu
  • Ping Zhou
  • Jingwen Dai
  • Nuonan Li
  • Hongfan Tao
  • Zhigang Ma
  • Zhongfang Zhang
Rapid Communication


Salinity (NaCl) was used in waste activated sludge (WAS) anaerobic fermentation system which had been presented to greatly enhance the extracellular polymeric substance (EPS) production including protein and polysaccharide and short-chain fatty acids (SCFAs). Salinity enhanced soluble protein and polysaccharide (SB-EPS) release which was 4.04 times (protein) and 1.83 times (polysaccharide) compared to 0 g/L NaCl level. More important, salinity restrained the coenzyme 420 activity (F420), but increased the hydrolase activity. Abundant hydrolysis of substrate and highly active hydrolase led to abundant SCFA production. Pearson correlation coefficient showed that the protein became the main reaction substrate for SCFA generation.

Graphic abstract


Waste activated sludge Salinity Extracellular polymeric substances Biological enzyme 



The authors are grateful to the doctoral foundation and incubation project of Zhengzhou University of Light Industry (13501050052, 2018ZCKJ201), also supported by the foundation of Henan province natural science (182300410140).


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Baodan Jin
    • 1
    Email author
  • Jintao Niu
    • 2
  • Ju Zhang
    • 1
  • Jiahui Niu
    • 1
  • Ping Zhou
    • 1
  • Jingwen Dai
    • 1
  • Nuonan Li
    • 1
  • Hongfan Tao
    • 1
  • Zhigang Ma
    • 1
  • Zhongfang Zhang
    • 1
  1. 1.Henan Collaborative Innovation Center of Environmental Pollution Control and Ecological Restoration, School of Material and Chemical EngineeringZhengzhou University of Light IndustryZhengzhouChina
  2. 2.Henan Hengan Environmental Protection Technology Co., Ltd.ZhengzhouChina

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