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Trametes versicolor laccase production using agricultural wastes: a comparative study in Erlenmeyer flasks, bioreactor and tray

  • Vanessa Elisa Pinheiro
  • Michele Michelin
  • Ana Claudia Vici
  • Paula Zaghetto de Almeida
  • Maria de Lourdes Teixeira de Moraes PolizeliEmail author
Research Paper
  • 76 Downloads

Abstract

Laccases are very interesting biocatalysts of recognized importance for several industrial applications. Its production by Trametes versicolor, a white-rot fungus, was induced by a combination of cotton gin wastes (1%), a lignocellulosic waste, and vinasse (15%), an industrial by-product from sugarcane industry. The use of these agro-industrial wastes are interesting, since it helps in reducing the enzyme production costs, due to their low cost and wide availability, as well as the environmental contamination issues, due to their improper disposal. Thus, laccase production was studied in submerged fermentation of T. versicolor using these agro-industrial wastes (cotton gin waste and vinasse) as carbon source and an additional nitrogen source (0.1% peptone). Three different bioreactors were evaluated for laccase production, such as BioFlo 310 bioreactor, aluminium tray and Erlenmeyer flasks to achieve high levels of laccase production. The highest specific production of laccase was found in BioFlo 310 bioreactor with 12 days of fermentation (55.24 U/mg prot.), which has been shown to be closely related to the oxygen supply to the microorganism through aeration of the fermentation medium. This study brings new insights into green biotechnology regarding vinasse utilization, which is frequently discharged in soils, rivers, and lakes causing adverse effects on agricultural soils and biota, as well as the cotton gin waste recovery.

Keywords

Laccase Trametes versicolor Vinasse Cotton gin wastes Aerated bioreactor Tray 

Notes

Acknowledgements

The authors are grateful to Mariana Cereia and Maurício de Oliveira for their technical assistance. This work was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, processes 2010/52322-3; 2014/50884-5; 2018/07522-6), and V. E. P. was the recipient of a FAPESP fellowship (Process 2015/23200-0).

Compliance with ethical standards

Conflict of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the manuscript.

Supplementary material

449_2019_2245_MOESM1_ESM.docx (1.5 mb)
Supplementary material 1 (DOCX 1559 kb)

References

  1. 1.
    Madhu A, Chakraborty JN (2017) Developments in application of enzymes for textile processing. J Clean Prod 145:114–133CrossRefGoogle Scholar
  2. 2.
    Srivastava A, Srivastava M (2018) Enzymes market type. https://www.alliedmarketresearch.com/request-sample/708. Accessed 03 Sept 2018
  3. 3.
    Bravo CEC, De Carvalho EP, Schwan RF, Gómez RJHC, Pilon L (2000) Determinação de condições ideais para produção de poligalacturonase por Kluyveromyces marxianus. Ciênc Agrotec 24:137–152Google Scholar
  4. 4.
    Sirotek K, Marounek M, Rada V, Benda V (2001) Isolation and characterization of rabbit caecal pectinolytic bacteria. Folia Microbiol 46:79–82CrossRefGoogle Scholar
  5. 5.
    Anisa SK, Girish K (2014) Pectinolytic activity of Rhizopus sp., and Trichoderma viride. J Pure Appl Microbiol 4:28–31Google Scholar
  6. 6.
    Sigoillot JC, Berrin JG, Bey M, Lesage-Meessen L, Levasseur A, Lomascolo A, Record E, Uzan-Boukhris E (2012) Lignins: biosynthesis, biodegradation and bioengineering. Adv Bot Res 61:263–308CrossRefGoogle Scholar
  7. 7.
    Martínkova L, Kotik M, Marková E, Homolka L (2016) Biodegradation of phenolic compounds by basidiomycota and its phenol oxidases: a review. Chemosphere 149:373–382CrossRefGoogle Scholar
  8. 8.
    Market and Markets (2016) Industrial enzymes market by type (amylases, cellulases, proteases, lipases, and phytases), application (food & beverages, cleaning agents, and animal feed), source (microorganism, plant, and animal), and region—global forecast to 2022. http://www.marketsandmarkets.com/Market-Reports/industrial-enzymes-market-237327836.html Accessed 22 Feb 2017
  9. 9.
    Spier MR, de Vandenberghe LPS, Medeiros ABP, Soccol CR (2011) Application of different types of bioreactors in bioprocesses. In: Antolli PG, Liu Z (eds) Bioreactors: design, properties and applications. New York, Nova Science Publishers Inc, pp 55–90Google Scholar
  10. 10.
    Alves F (2010) Modelagem e simulação de biorreator operando com fungo Trametes versicolor para produção de enzima lacase. Masters dissertation—Escola de Engenharia Mauá—Centro Universitário do Instituto Mauá de TecnologiaGoogle Scholar
  11. 11.
    Stanbury PF (1995) Principles of fermentation technology. Pergamon Press, OxfordGoogle Scholar
  12. 12.
    Cavalcanti JEWA (2009) Manual de tratamento de efluentes industriais. Engenho Editora técnica Ltda, São PauloGoogle Scholar
  13. 13.
    NOVACANA (2013) Uso da vinhaça na cultura da cana-de-açúcar. https://www.novacana.com/cana/uso-vinhaca-cultura Accessed 22 Mar 2017
  14. 14.
    Silva APM, Bono JAM, Pereira FAR (2014) Fertigation with vinasse in sugarcane crop: effect on the soil and on productivity. Rev Bras Eng Agric Amb 18:38–43CrossRefGoogle Scholar
  15. 15.
    Christofoletti CA, Escher JP, Correia JE, Marinho JFU, Fontanetti CS (2013) Sugarcane vinasse: environmental implications of its use. J Waste Manag 33:2752–2761CrossRefGoogle Scholar
  16. 16.
    Kahraman SS, Gurdal IH (2002) Effect of synthetic and natural culture media on laccase production by white rot fungi. Bioresour Technol 82:215–217CrossRefGoogle Scholar
  17. 17.
    Boran F, Yesilada O (2011) Enhanced production of laccase by fungi under solid substrate fermentation condition. BioResources 6:4404–4416Google Scholar
  18. 18.
    España-Gamboa E, Vicent T, Font X, Dominguez-Maldonado J, Canto-Canché B, Alzate-Gaviria L (2017) Pretreatment of vinasse from the sugar refinery industry under non-sterile conditions by Trametes versicolor in a fluidized bed bioreactor and its effect when coupled to an UASB reactor. J Biol Eng 11:1–11CrossRefGoogle Scholar
  19. 19.
    Rizzatti ACS, Jorge JA, Terenzi HF, Rechia CGV, Polizeli MLTM (2001) Purification and properties of a thermostable extracellular β-dxylosidase produced by thermotolerant Aspergillus phoenicis. J Ind Microbiol Biotechnol 26:156–160CrossRefGoogle Scholar
  20. 20.
    Buswell JK, Cai YJ, Chang ST (1995) Effect of nutrient nitrogen on manganese peroxidase and laccase production by Lentinula (Lentinus) edodes. FEMS Microbiol Lett 128:81–88CrossRefGoogle Scholar
  21. 21.
    Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254CrossRefGoogle Scholar
  22. 22.
    Nicochelli LM (2011) Sorção ao potássio de diferentes materiais submetidos à aplicação de vinhaça. Master dissertation—Universidade Federal de Mato Grosso, CuiabáGoogle Scholar
  23. 23.
    Prado RM, Caione G, Campos CNF (2013) Filter cake and vinasse as fertilizers contributing to conservation agriculture. Appl Environ Soil Sci 2013:1–8CrossRefGoogle Scholar
  24. 24.
    Agblevor FA, Batz S, Trumbo J (2003) Composition and ethanol production potential of cotton gin residues. Appl Biochem Biotechnol 105–108:219–230CrossRefGoogle Scholar
  25. 25.
    Hsieh Y (2007) Chemical structure and properties of cotton. Cotton Sci Technol.  https://doi.org/10.1533/9781845692483.1.3 CrossRefGoogle Scholar
  26. 26.
    De Souza ÉS, Sampaio ID, Freire AKD, Da Silva BKS, Sobrinho AD, Lima AM, Souza JVB (2011) Production of Trametes versicolor laccase by solid state fermentation using a fixed-bed bioreactor. JFAE 9:55–58Google Scholar
  27. 27.
    Rodríguez Couto S, Moldes D, Liébanas A, Sanromán Á (2003) Investigation of several bioreactor configurations for laccase production by Trametes versicolor operating in solid-state conditions. Biochem Eng J 6:15–21Google Scholar
  28. 28.
    Levin L, Melignani E, Ramos AM (2010) Effect of nitrogen sources and vitamins on ligninolytic enzyme production by some white-rot fungi. Dye decolorization by selected culture filtrates. Bioresour Technol 101:4554–4563CrossRefGoogle Scholar
  29. 29.
    Wang F, Hu JH, Guo C, Liu CZ (2014) Enhanced laccase production by Trametes versicolor using corn steep liquor as both nitrogen source and inducer. Bioresour Technol 166:62–65Google Scholar
  30. 30.
    Mikiashvili N, Elisashvili V, Wasser S, Nevo E (2005) Carbon and nitrogen sources influence the ligninolytic enzyme activity of Trametes versicolor. Biotechnol Lett 27:955–959CrossRefGoogle Scholar
  31. 31.
    Galhaup C, Wagner H, Hinterstoisser B, Haltrich D (2002) Increased production of laccase by the wood-degrading basidiomycete Trametes pubescens. Enzyme Microb Technol 30:529–536CrossRefGoogle Scholar
  32. 32.
    Collins PJ, Dobson ADW (1997) Regulation of laccase gene transcription in Trametes versicolor. Appl Environ Microbiol 63:3444–3450PubMedPubMedCentralGoogle Scholar
  33. 33.
    Cascaval D, Galaction A-I, Turnea M (2011) Comparative analysis of oxygen transfer rate distribution in stirred bioreactor for simulated and real fermentation broths. J Ind Microbiol Biotechnol 38:1449–1466CrossRefGoogle Scholar
  34. 34.
    Michelin M, Mota AMO, Polizeli MLTM, Silva DP, Vicente AA, Teixeira JA (2013) Influence of volumetric oxygen transfer coefficient (kLa) on xylanases batch production by Aspergillus niger van Tieghem in stirred tank and internal-loop airlift bioreactors. Biochem Eng J 80:19–26CrossRefGoogle Scholar
  35. 35.
    Ghoshal K, Bhattacharyya K (2014) Overview of platelet physiology: its hemostatic and nonhemostatic role in disease pathogenesis. Sci World J 2014:1–16CrossRefGoogle Scholar
  36. 36.
    Fontana RC, Silveira MM (2012) Production of polygalacturonases by Aspergillus oryzae in stirred tank and internal—and external-loop airlift reactors. Bioresour Technol 123:157–163CrossRefGoogle Scholar
  37. 37.
    Mainardi PH, Feitosa VA, De Paiva LBB, Bonugli-Santos RC, Squina FM, Pessoa A Jr, Sette LD (2018) Laccase production in bioreactor scale under saline condition by the marine-derived basidiomycete Peniophora Sp. CBMAI 1063. Fungal Biol 122:302–309CrossRefGoogle Scholar
  38. 38.
    El-Batal AI, ElKenawy NM, Yassin AS, Amin MA (2015) Laccase production by Pleurotus ostreatus and its application in synthesis of gold nanoparticles. Biotechnol Rep 5:31–39CrossRefGoogle Scholar
  39. 39.
    Adekunle AE, Zhang C, Guo C, Liu C (2016) Laccase production from Trametes versicolor in solid-state fermentation of steam-exploded pretreated corn stalk. Waste Biomass Valoriz.  https://doi.org/10.1007/s12649-016-9562-9 CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Departamento de Bioquímica e ImunologiaUniversidade de São Paulo (USP), Faculdade de Medicina de Ribeirão PretoRibeirão PretoBrazil
  2. 2.Centre of Biological EngineeringUniversity of MinhoBragaPortugal
  3. 3.Departamento de BiologiaUniversidade de São Paulo (USP), Faculdade de Filosofia, Ciências e Letras de Ribeirão PretoRibeirão PretoBrazil

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