Trametes versicolor laccase production using agricultural wastes: a comparative study in Erlenmeyer flasks, bioreactor and tray
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Laccases are very interesting biocatalysts of recognized importance for several industrial applications. Its production by Trametes versicolor, a white-rot fungus, was induced by a combination of cotton gin wastes (1%), a lignocellulosic waste, and vinasse (15%), an industrial by-product from sugarcane industry. The use of these agro-industrial wastes are interesting, since it helps in reducing the enzyme production costs, due to their low cost and wide availability, as well as the environmental contamination issues, due to their improper disposal. Thus, laccase production was studied in submerged fermentation of T. versicolor using these agro-industrial wastes (cotton gin waste and vinasse) as carbon source and an additional nitrogen source (0.1% peptone). Three different bioreactors were evaluated for laccase production, such as BioFlo 310 bioreactor, aluminium tray and Erlenmeyer flasks to achieve high levels of laccase production. The highest specific production of laccase was found in BioFlo 310 bioreactor with 12 days of fermentation (55.24 U/mg prot.), which has been shown to be closely related to the oxygen supply to the microorganism through aeration of the fermentation medium. This study brings new insights into green biotechnology regarding vinasse utilization, which is frequently discharged in soils, rivers, and lakes causing adverse effects on agricultural soils and biota, as well as the cotton gin waste recovery.
KeywordsLaccase Trametes versicolor Vinasse Cotton gin wastes Aerated bioreactor Tray
The authors are grateful to Mariana Cereia and Maurício de Oliveira for their technical assistance. This work was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, processes 2010/52322-3; 2014/50884-5; 2018/07522-6), and V. E. P. was the recipient of a FAPESP fellowship (Process 2015/23200-0).
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Conflict of interest
The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the manuscript.
- 2.Srivastava A, Srivastava M (2018) Enzymes market type. https://www.alliedmarketresearch.com/request-sample/708. Accessed 03 Sept 2018
- 3.Bravo CEC, De Carvalho EP, Schwan RF, Gómez RJHC, Pilon L (2000) Determinação de condições ideais para produção de poligalacturonase por Kluyveromyces marxianus. Ciênc Agrotec 24:137–152Google Scholar
- 5.Anisa SK, Girish K (2014) Pectinolytic activity of Rhizopus sp., and Trichoderma viride. J Pure Appl Microbiol 4:28–31Google Scholar
- 8.Market and Markets (2016) Industrial enzymes market by type (amylases, cellulases, proteases, lipases, and phytases), application (food & beverages, cleaning agents, and animal feed), source (microorganism, plant, and animal), and region—global forecast to 2022. http://www.marketsandmarkets.com/Market-Reports/industrial-enzymes-market-237327836.html Accessed 22 Feb 2017
- 9.Spier MR, de Vandenberghe LPS, Medeiros ABP, Soccol CR (2011) Application of different types of bioreactors in bioprocesses. In: Antolli PG, Liu Z (eds) Bioreactors: design, properties and applications. New York, Nova Science Publishers Inc, pp 55–90Google Scholar
- 10.Alves F (2010) Modelagem e simulação de biorreator operando com fungo Trametes versicolor para produção de enzima lacase. Masters dissertation—Escola de Engenharia Mauá—Centro Universitário do Instituto Mauá de TecnologiaGoogle Scholar
- 11.Stanbury PF (1995) Principles of fermentation technology. Pergamon Press, OxfordGoogle Scholar
- 12.Cavalcanti JEWA (2009) Manual de tratamento de efluentes industriais. Engenho Editora técnica Ltda, São PauloGoogle Scholar
- 13.NOVACANA (2013) Uso da vinhaça na cultura da cana-de-açúcar. https://www.novacana.com/cana/uso-vinhaca-cultura Accessed 22 Mar 2017
- 17.Boran F, Yesilada O (2011) Enhanced production of laccase by fungi under solid substrate fermentation condition. BioResources 6:4404–4416Google Scholar
- 18.España-Gamboa E, Vicent T, Font X, Dominguez-Maldonado J, Canto-Canché B, Alzate-Gaviria L (2017) Pretreatment of vinasse from the sugar refinery industry under non-sterile conditions by Trametes versicolor in a fluidized bed bioreactor and its effect when coupled to an UASB reactor. J Biol Eng 11:1–11CrossRefGoogle Scholar
- 22.Nicochelli LM (2011) Sorção ao potássio de diferentes materiais submetidos à aplicação de vinhaça. Master dissertation—Universidade Federal de Mato Grosso, CuiabáGoogle Scholar
- 26.De Souza ÉS, Sampaio ID, Freire AKD, Da Silva BKS, Sobrinho AD, Lima AM, Souza JVB (2011) Production of Trametes versicolor laccase by solid state fermentation using a fixed-bed bioreactor. JFAE 9:55–58Google Scholar
- 27.Rodríguez Couto S, Moldes D, Liébanas A, Sanromán Á (2003) Investigation of several bioreactor configurations for laccase production by Trametes versicolor operating in solid-state conditions. Biochem Eng J 6:15–21Google Scholar
- 29.Wang F, Hu JH, Guo C, Liu CZ (2014) Enhanced laccase production by Trametes versicolor using corn steep liquor as both nitrogen source and inducer. Bioresour Technol 166:62–65Google Scholar