Trametes versicolor laccase production using agricultural wastes: a comparative study in Erlenmeyer flasks, bioreactor and tray

  • Vanessa Elisa Pinheiro
  • Michele Michelin
  • Ana Claudia Vici
  • Paula Zaghetto de Almeida
  • Maria de Lourdes Teixeira de Moraes PolizeliEmail author
Research Paper


Laccases are very interesting biocatalysts of recognized importance for several industrial applications. Its production by Trametes versicolor, a white-rot fungus, was induced by a combination of cotton gin wastes (1%), a lignocellulosic waste, and vinasse (15%), an industrial by-product from sugarcane industry. The use of these agro-industrial wastes are interesting, since it helps in reducing the enzyme production costs, due to their low cost and wide availability, as well as the environmental contamination issues, due to their improper disposal. Thus, laccase production was studied in submerged fermentation of T. versicolor using these agro-industrial wastes (cotton gin waste and vinasse) as carbon source and an additional nitrogen source (0.1% peptone). Three different bioreactors were evaluated for laccase production, such as BioFlo 310 bioreactor, aluminium tray and Erlenmeyer flasks to achieve high levels of laccase production. The highest specific production of laccase was found in BioFlo 310 bioreactor with 12 days of fermentation (55.24 U/mg prot.), which has been shown to be closely related to the oxygen supply to the microorganism through aeration of the fermentation medium. This study brings new insights into green biotechnology regarding vinasse utilization, which is frequently discharged in soils, rivers, and lakes causing adverse effects on agricultural soils and biota, as well as the cotton gin waste recovery.


Laccase Trametes versicolor Vinasse Cotton gin wastes Aerated bioreactor Tray 



The authors are grateful to Mariana Cereia and Maurício de Oliveira for their technical assistance. This work was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, processes 2010/52322-3; 2014/50884-5; 2018/07522-6), and V. E. P. was the recipient of a FAPESP fellowship (Process 2015/23200-0).

Compliance with ethical standards

Conflict of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the manuscript.

Supplementary material

449_2019_2245_MOESM1_ESM.docx (1.5 mb)
Supplementary material 1 (DOCX 1559 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Departamento de Bioquímica e ImunologiaUniversidade de São Paulo (USP), Faculdade de Medicina de Ribeirão PretoRibeirão PretoBrazil
  2. 2.Centre of Biological EngineeringUniversity of MinhoBragaPortugal
  3. 3.Departamento de BiologiaUniversidade de São Paulo (USP), Faculdade de Filosofia, Ciências e Letras de Ribeirão PretoRibeirão PretoBrazil

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