Advertisement

Acetylcholinesterase biosensors based on ionic liquid functionalized carbon nanotubes and horseradish peroxidase for monocrotophos determination

  • 72 Accesses

Abstract

Long-term and excessive use of monocrotophos (MPs) pesticide leads to an accumulation of MPs residues in agricultural products. Electrochemical biosensor technology was developed as a simple and efficient method for detecting MPs. However, commercial acetylcholinesterase (AChE) sensors are not applied in practical MPs detection due to poor stability and reliability. In this study, the advantages of functionalized carbon nanotubes (Cl/MWCNTs) and a bi-enzyme system (horseradish peroxidase (HRP)/AChE) were combined, a novel bi-enzyme electrode (Cl/MWCNTs/HRP/AChE/GCE) was constructed. Under optimal conditions, the bi-enzyme sensor had a wide detection range of 1.0 × 10–11 to 1.0 × 10–7 mol/L and low detection limit of 4.5 × 10–12 mol/L. The proposed AChE biosensor exhibited excellent stability and sensitivity for MPs determination and presented a promising tool for monitoring food safety.

This is a preview of subscription content, log in to check access.

Access options

Buy single article

Instant unlimited access to the full article PDF.

US$ 39.95

Price includes VAT for USA

Subscribe to journal

Immediate online access to all issues from 2019. Subscription will auto renew annually.

US$ 199

This is the net price. Taxes to be calculated in checkout.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

References

  1. 1.

    Satnami M, Korram J, Nagwanshi R, Vaishnav SK, Karbhal I, Dewangan H (2018) Gold nanoprobe for inhibition and reactivation of acetylcholinesterase: an application to detection of organophosphorus pesticides. Sensor Actuat B Chem 267:155–164

  2. 2.

    Blennow K, De Leon MJ, Zetterberg H (2006) Alzheimer’s disease. Lancet 368:387–403

  3. 3.

    Aybastıer Ö, Dawbaa S, Demir C, Akgün O, Ulukaya E, Arı F (2018) Quantification of DNA damage products by gas chromatography tandem mass spectrometry in lung cell lines and prevention effect of thyme antioxidants on oxidative induced DNA damage. Mutat Res Fund Mol Mech Mutagen 808:1–9

  4. 4.

    Desbat B, Turlet JM, Buffeteau T (2017) Polarization modulation ft-ir spectroscopy of surfaces and ultra-thin films: experimental procedure and quantitative analysis. Appl Spectrosc 45:380–389

  5. 5.

    Chen D, Liu Z, Fu J, Guo Y, Sun X, Yang Q, Wang X (2017) Electrochemical acetylcholinesterase biosensor based on multi-walled carbon nanotubes/dicyclohexyl phthalate modified screen-printed electrode for detection of chlorpyrifos. J Electroanal Chem 801:185–191

  6. 6.

    Zhang Q, Hou W, Li F, Zhang Y, Guo Y, Sun X (2019) Acetylcholinesterase biosensor odified with ATO/OMC for detecting organophosphorus pesticides. N J Chem 43:946–952

  7. 7.

    Fu J, Zhang Q, Shi Z, Guo Y, Li F, Zhang Y, Xia S (2018) Sensitive acetylcholinesterase biosensor based on screen-printed carbon electrode modified with cerium oxide-chitosan/mesoporous carbon-chitosan for organophosphorus pesticide residue detection. Int J Electrochem Sci 13:9231–9241

  8. 8.

    Dhull V (2018) Fabrication of ache/SnO2-cmwcnts/cu nanocomposite-based sensor electrode for detection of methyl parathion in water. Int J Anal Chem 2018:1–7

  9. 9.

    Gumpu MB, Veerapandian M, Krishnan UM, Rayappan J (2018) Amperometric determination of as(iii) and cd(ii) using a platinum electrode modified with acetylcholinesterase, ruthenium(ii)-tris(bipyridine) and graphene oxide. Microchim Acta 185:297–308

  10. 10.

    Liu ZJ (2007) Review: china's geography: globalization and the dynamics of political, economic, and social change. Eurasian Geogr Econ 48:769–773

  11. 11.

    Vikas D (2018) Nafion/AChE-cSWCNT/MWCNT/Au based amperometric biosensor for determination of organophosphorous compounds. Environ Technol 33:1–23

  12. 12.

    Çevik S, Timur S, Aniku Ü (2013) A poly(allylamine hydrochloride) functionalized multiwalled carbon nanotube modified carbon paste electrode as acetylcholinesterase biosensor transducer. Electroanal 25:2377–2383

  13. 13.

    Zou B, Chu Y, Xia J, Yao J (2018) Acetylcholinesterase biosensor based on functionalized surface of carbon nanotubes for monocrotophos detection. Anal Biochem 560:12–18

  14. 14.

    Chauhan N, Pundir CS (2011) An amperometric biosensor based on acetylcholinesterase immobilized onto iron oxide nanoparticles/multi-walled carbon nanotubes modified gold electrode for measurement of organophosphorus insecticides. Anal Chim Acta 701:66–74

  15. 15.

    Baker PA, Goltz MN, Schrand AM, Yoon DY, Kim DS (2014) Organophosphate vapor detection on gold electrodes using peptide nanotubes. Biosens Bioelectron 61:119–123

  16. 16.

    Liu B, Lu L, Liu C (2011) Immunosensor for myeloperoxidase based on PoPD-MWCNTs-ionic liquid/nanogold modified Au electrode. Acta Chim Sin 69:438–444

  17. 17.

    Cui H, Wu W, Li M, Song X, Lv Y, Zhang T (2017) A highly stable acetylcholinesterase biosensor based on chitosan-TiO2-graphene nanocomposites for detection of organophosphate pesticides. Biosens Bioelectron 99:223–229

  18. 18.

    Bidari A, Ganjali MR, Norouzi P (2011) Sample preparation method for the analysis of some organophosphorus pesticides residues in tomato by ultrasound-assisted solvent extraction followed by dispersive liquid-liquid microextraction. Food Chem 126:1840–1844

  19. 19.

    Rodrigues N, Sakae N, Rita L, Damos F, Hideko Y (2018) Ultrasensitive determination of malathion using acetylcholinesterase immobilized on chitosan-functionalized magnetic iron nanoparticles. Biosensors 8:16–27

  20. 20.

    Ezhilan M, Gumpu MB, Ramachandra BL, Nesakumar N, Babu KJ, Krishnan UM (2017) Design and development of electrochemical biosensor for the simultaneous detection of melamine and urea in adulterated milk samples. Sens Actuators B Chem 238:1283–1292

  21. 21.

    Song D, Li Y, Lu X, Sun M, Liu H, Yu G (2017) Palladium-copper nanowires-based biosensor for the ultrasensitive detection of organophosphate pesticides. Anal Chim Acta 982:168–175

  22. 22.

    Guo Y, Chen W, Xiong Q, Ren Q, Sun L, Han B (2017) Chemically modified multiwalled carbon nanotubes improve the cytocompatibility. Mater Res Express 4:125801–125808

  23. 23.

    Jeyanthi P, Ramalingam C (2019) Acetylcholinesterase with mesoporous silica: covalent immobilization, physiochemical characterization, and its application in food for pesticide detection. J Cell Biochem 120:1–10

  24. 24.

    Wang S, Zhao P, Li N, Qiao X, Xu Z (2017) Development of a chemiluminescence sensor based on molecular imprinting technology for the determination of trace monocrotophos in vegetables. Adv Polym Tech 37:1–9

  25. 25.

    Zou B, Chu Y, Xia J (2019) Monocrotophos detection with a bienzyme biosensor based on ionic-liquid-modified carbon nanotubes. Anal Bioanal Chem 411:2905–2914

  26. 26.

    Tseng F, Lin K, Hsu H (2004) A surface-tension-driven fluidic network for precise enzyme batch-dispensing and glucose detection. Sensor Actuators A phys 111:107–117

Download references

Acknowledgements

The work was funded by the National Natural Science Foundation of China (No. 21406093), the Natural Science Foundation of Jiangsu province (BK20140529), the Open Project Program of State Key Laboratory of Food Science and Technology of Jiangnan University (SKLF-KF-201919), Key University Science Research Project of Jiangsu Province (14KJB530001), China Postdoctoral Science Foundation (2014M550271), and Priority Academic Program Development of Jiangsu Higher Education Institutions.

Author information

Correspondence to Zou Bin.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Jiaojiao, X., Bin, Z., Pengyun, W. et al. Acetylcholinesterase biosensors based on ionic liquid functionalized carbon nanotubes and horseradish peroxidase for monocrotophos determination. Bioprocess Biosyst Eng 43, 293–301 (2020). https://doi.org/10.1007/s00449-019-02226-2

Download citation

Keywords

  • Monocrotophos
  • Carbon nanotubes
  • Horseradish peroxidase
  • Acetylcholinesterase
  • Biosensor