Acetylcholinesterase biosensors based on ionic liquid functionalized carbon nanotubes and horseradish peroxidase for monocrotophos determination

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Long-term and excessive use of monocrotophos (MPs) pesticide leads to an accumulation of MPs residues in agricultural products. Electrochemical biosensor technology was developed as a simple and efficient method for detecting MPs. However, commercial acetylcholinesterase (AChE) sensors are not applied in practical MPs detection due to poor stability and reliability. In this study, the advantages of functionalized carbon nanotubes (Cl/MWCNTs) and a bi-enzyme system (horseradish peroxidase (HRP)/AChE) were combined, a novel bi-enzyme electrode (Cl/MWCNTs/HRP/AChE/GCE) was constructed. Under optimal conditions, the bi-enzyme sensor had a wide detection range of 1.0 × 10–11 to 1.0 × 10–7 mol/L and low detection limit of 4.5 × 10–12 mol/L. The proposed AChE biosensor exhibited excellent stability and sensitivity for MPs determination and presented a promising tool for monitoring food safety.

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The work was funded by the National Natural Science Foundation of China (No. 21406093), the Natural Science Foundation of Jiangsu province (BK20140529), the Open Project Program of State Key Laboratory of Food Science and Technology of Jiangnan University (SKLF-KF-201919), Key University Science Research Project of Jiangsu Province (14KJB530001), China Postdoctoral Science Foundation (2014M550271), and Priority Academic Program Development of Jiangsu Higher Education Institutions.

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Correspondence to Zou Bin.

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Jiaojiao, X., Bin, Z., Pengyun, W. et al. Acetylcholinesterase biosensors based on ionic liquid functionalized carbon nanotubes and horseradish peroxidase for monocrotophos determination. Bioprocess Biosyst Eng 43, 293–301 (2020).

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  • Monocrotophos
  • Carbon nanotubes
  • Horseradish peroxidase
  • Acetylcholinesterase
  • Biosensor