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Embedding live bacteria in porous hydrogel/ceramic nanocomposites for bioprocessing applications

  • Jessica Condi Mainardi
  • Kurosch Rezwan
  • Michael MaasEmail author
Research Paper
  • 61 Downloads

Abstract

In this work, we present a biocompatible one-pot processing route for ceramic/hydrogel nanocomposites in which we embed live bacteria. In our approach, we fabricate a highly stable alginate hydrogel with minimal shrinkage, highly increased structural and mechanical stability, as well as excellent biocompatibility. The hydrogel was produced by ionotropic gelation and reinforced with alumina nanoparticles to form a porous 3D network. In these composite gels, the bacteria Escherichia coli and Bacillus subtilis were embedded. The immobilized bacteria showed high viability and similar metabolic activity as non-embedded cells. Even after repeated glucose consumption cycles, the material maintained high structural stability with stable metabolic activity of the immobilized bacteria. Storing the bionanocomposite for up to 60 days resulted in only minor loss of activity. Accordingly, this approach shows great potential for producing macroscopic bioactive materials for biotechnological processes.

Keywords

Cell encapsulation Nanocomposite Hydrogel Ceramic nanoparticles 

Notes

Acknowledgements

We would like to thank DFG Research Training Group GRK 1860, ‘Micro-, meso- and macroporous nonmetallic materials: fundamentals and applications’ (MIMENIMA) for funding.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

449_2019_2119_MOESM1_ESM.doc (107 kb)
Supplementary material 1 (DOC 107 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Keramische Werkstoffe und Bauteile, Advanced Ceramics, Universität BremenBremenGermany
  2. 2.MAPEX Center for Materials and ProcessesUniversity of BremenBremenGermany

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