Functionalized kaolin as support for endoglucanase immobilization
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Endoglucanases are an enzyme of cellulases complex that has a great potential for many technological applications. One of the issues of its use concerns the recovery and reuse of this enzyme. Thus, in this study, the use of a surface-modified kaolin was evaluated to immobilize endoglucanase and evaluate the enzyme activity for its reuse. Kaolin was surface modified with 3-aminopropyltriethoxysilane (APTES) and glutaraldehyde (GA). In addition, the properties of the immobilized enzyme were investigated and compared with those of the free enzyme. Results showed that the optimal pH value of endoglucanase was not affected by the immobilization process but showed a broader range of optimal temperature compared to free enzyme. Immobilization on kaolin allowed fast and easy cellulase recovery with a loss of enzyme activity of only 20% after eight cycles of use. These results indicate that kaolin is a promising substitute to the currently synthetic supports studied for cellulases immobilization with the advantage of being abundant in nature, resistant to microbial attack, chemically and mechanically stable.
KeywordsEndoglucanase Immobilized cellulase Enzyme immobilization Kaolin
The authors would like to thank Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for financial support, as well as Laboratório Central de Microscopia Eletrônica at UFSC (LCME-UFSC) for TEM and SEM analyses.
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