Integrated thermophilic enzyme-immobilized reactor and high-rate biological reactors for treatment of palm oil-containing wastewater without sludge production

  • P. SaranyaEmail author
  • P. K. Selvi
  • G. SekaranEmail author
Research Paper


Bacillus pumilus, the thermophilic microorganism, was used to biosynthesise lipase of specific activity 5173 U. The purified lipase was highly stable in the pH range from 1 to 7 and temperature at 50 °C. The functionalized nanoporous activated carbon matrix was used for the immobilization of lipase at the optimum conditions and was used for the hydrolysis of palm oil-containing wastewater at optimum time, 3 h, pH 7, and at temperature 50 °C. The hydrolyzed palm oil wastewater was treated in an upflow anaerobic reactor for the removal of soluble COD at hydraulic retention time (HRT) of 3 days. The anaerobic-treated wastewater was applied to the fluidized immobilized carbon catalytic oxidation (FICCO) reactor at HRT of 16 h to reduce the soluble COD. The FICCO-treated wastewater was further treated in chemo-autotrophic activated carbon oxidation (CAACO) reactor to reduce the COD less than 100 mg/L.


Lipase Hydrolysis Functionalized nanoporous activated carbon Upflow anaerobic reactor FICCO CACCO 



Author Dr. P. Saranya is grateful to the Council of Scientific and Industrial Research (CSIR), India.

Supplementary material

449_2019_2104_MOESM1_ESM.docx (155 kb)
Supplementary material 1 (DOCX 154 KB)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Ministry of Environment, Forest and Climate Change, Indira Paryavaran BhawanNew DelhiIndia
  2. 2.Central Pollution Control Board (Ministry of Environment, Forest and Climate Change), Parivesh BhawanNew DelhiIndia
  3. 3.Environmental Science and EngineeringCSIR-Central Leather Research InstituteChennaiIndia

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