Microalga Scenedesmus bajacalifornicus BBKLP-07, a new source of bioactive compounds with in vitro pharmacological applications

  • Lakkanagouda Patil
  • B. B. KaliwalEmail author
Research Paper


Microalgae are photosynthetic eukaryotes which are primary producers in the food chain and also excellent sources for bioactive compounds such as alkaloids, flavonoids, phenols, saponins and other fine chemicals. In the present study, the microalga Scenedesmus bajacalifornicus BBKLP-07 was subjected to soxhlet extraction using solvents like chloroform, acetone, ethanol, methanol and aqueous solvents. All the solvents were tested for the presence of phytochemical constituents such as alkaloids, flavonoids, glycosides, phenols, lignin’s, saponins, sterols, tannins, anthraquinone and reducing sugar using the standard procedures. Furthermore, all the crude extracts were subjected to antidiabetic, antioxidant, anti-inflammatory and antimicrobial activities. Antidiabetic activity of the microalgal extracts was observed maximum in Aqueous extract. Methanolic extracts have shown maximum antioxidant activity and chloroform extracts have exhibited highest anti-inflammatory effects. Antimicrobial activities were tested against E.coli, S, typhi, C.perfringens and B.subtilis bacteria and fungi A.niger, and C. albicans. Therefore, the green microalga Scenedesmus bajacalifornicus BBKLP-07 is a rich source of biological active compounds and nutraceuticals and can be exploited for commercial applications.


Scenedesmus bajacalifornicus Microalgae Antidiabetic Anti-inflamatory Antimicrobial Phytochemical screening 



Authors are profusely thankful to the Department of Biotechnology (DBT), Ministry of Science and Technology, Government of India, New Delhi, for funding the Bioinformatics Infrastructure Facility Project (BT/BI/25/001/2006 VOL II date 05-03-2012) and also the Interdisciplinary Program for Life Science Project (BT/PR/4555/INF/22/126/2010 dated 30-09-2010) and P. G Departments of Biotechnology and Microbiology Karnatak University, Dharwad, for providing the facilities for pursuing the research work at the Department.

Compliance with ethical standards

Conflict of interest

Authors do not have any conflict of interest related to the manuscript.

Ethical approval

This article does not contain any studies related to animals and human participants.


  1. 1.
    Abd El Baky HH, El-Baroty GS (2013) Healthy benefit of microalgal bioactive substances. J Aquat Sci 1(1):11–23Google Scholar
  2. 2.
    Abedin RMA, Taha HM (2008) Antibacterial and antifungal activity of Cyanobacteria and Green Microalgae. Evaluation of medium components by Plackett–Burman design for antimicrobial activity of Spirulina Platensis. Glob J Biotechnol Biochem 3(1):22–31Google Scholar
  3. 3.
    Ann M, Zigang D (2013) Signal transduction and molecular targets of selected flavonoids. Antioxid Redox Signal 19(2):163 180Google Scholar
  4. 4.
    Baviskar JW, Khandelwal SR (2015) Extraction, detection and identification of flavonoids from microalgae: an emerging secondary metabolite. Int J Curr Microbiol App Sci 2:110–117Google Scholar
  5. 5.
    Bely A (2002) The potential application of Spirulina (Arthrospira) as a nutritional and therapeutic supplement in health management. JANA 5:27–48Google Scholar
  6. 6.
    Bhagavathy S, Sumathi P, Bell JS (2011) Green algae Chlorococcum humicola—a new source of bioactive compounds with antimicrobial activity. Asian Pac J Trop Med 1(1):S1–S7Google Scholar
  7. 7.
    Bhosale P (2004) Environmental and cultural stimulants in the production of carotenoids from microorganisms. Appl Microbiol Biotechnol 63:351–361Google Scholar
  8. 8.
    Carocho M, Ferreira IC (2013) A review on antioxidants, prooxidants and related controversy: natural and synthetic compounds, screening and analysis methodologies and future perspectives. Food Chem Toxicol 51:15–25Google Scholar
  9. 9.
    Catarina GA, Catarina RB, Helena MA, Claudia IP, Francisco XM (2011) Microalgal and cyanobacterial cell extracts for use as natural antibacterial additives against food pathogens. Int J Food Sci Technol 46(4):862–870Google Scholar
  10. 10.
    Chacon-Lee TL, González-Marino GE (2010) Microalgae for healthy foods–possibilities and challenges. Compr Rev Food Sci Food Saf 9(6):655–675Google Scholar
  11. 11.
    Chang C, Yang M, Wen H, Chern J (2002) Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal 10:178–182Google Scholar
  12. 12.
    Chu CY, Liao WR, Huang R, Lin LP (2004) Haemagglutinating and antibiotic activities of freshwater microalgae. World J Microbiol Biotechnol 20(8):817–825Google Scholar
  13. 13.
    Dillard CJ, Litov RE, Savin WM, Dumelin EE, Tappel AL (1978) Effects of exercise, vitamin-E, and ozone on pulmonary-function and lipid peroxidation. J Appl Physiol 45(6):927–932Google Scholar
  14. 14.
    Edeoga HO, Okwu DE, Mbaebie BO (2005) Phytochemical constituents of some Nigerian medicinal plants. Afr J Biotechnol 4:685–688Google Scholar
  15. 15.
    Ferreira LC, Grabe-Guimarães A, de Paula CA, Michel MCP, Guimarães RG, Rezende SA, Filho JDS, Saúde-Guimaraes DA (2013) Anti-inflammatory and antinociceptive activities of Campomanesia adamantium. J Ethnopharmacol 145:100–108Google Scholar
  16. 16.
    Guedes AG, Morisseau C, Sole A, Soares JH, Ulu A, Dong H, Hammock BD (2013) Use of a soluble epoxide hydrolase inhibitor as an adjunctive analgesic in a horse with laminitis. Vet Anaesth Analg 40(4):440–448Google Scholar
  17. 17.
    Guiry MD, Guiry G, M (2014) Algaebase. In: World-wide electronic publication. National University of Ireland, GalwayGoogle Scholar
  18. 18.
    Halliwell B (1996) Antioxidants in human health and disease. Ann Rev Nutr 16:33–50Google Scholar
  19. 19.
    Herrero M, Cifuentes. A, Ibáñez E (2006) Sub- and supercritical fluid extraction of functional ingredients from different natural sources: plants, food-by-products, algae and microalgae: a review. Food Chem 98:136–148Google Scholar
  20. 20.
    Hiremath GB, Kaliwal BB (2017) Phytochemical analysis and antimicrobial activity of rhizome extracts of Curcuma pseudomontana j. Graham. Int J Recent Sci Res 8(7):18890–18895Google Scholar
  21. 21.
    Ho SH, Chen CY, Chang JS (2012) Effect of light intensity and nitrogen starvation on CO2 fixation and lipid/carbohydrate production of an indigenous microalga Scenedesmus obliquus CNW-N. Bioresour Technol 113:244–252Google Scholar
  22. 22.
    Kavitha J, Palani S (2016) Phytochemical screening, Gc-Ms analysis and antioxidant activity af Marine Algae Chlorococcum humicola. WJPPS 5(6):1154–1167Google Scholar
  23. 23.
    Kuda T, Tsunekawa M, Hishi T, Araki Y (2005) Antioxidant properties of dried kayamo-nori, a brown alga Scytosiphon lomentaria (Scytosiphonales, Phaeophyceae). Food Chem 89:617–622Google Scholar
  24. 24.
    Lee JB, Hayashi K, Hirata M, Kuroda E, Suzuki E, Kubo Y (2006) Antiviral sulfated polysaccharide from Navicula directa, a diatom collected from deep-sea water in Toyama Bay. Biol Pharm Bull 29:2135–2139Google Scholar
  25. 25.
    Lee SH, Li Y, Karadeniz F (2009) α-Glucosidase and α-amylase inhibitory activities of phloroglucinal derivatives from edible marine brown alga. Ecklonia cava J Sci Food Agric 89:1552–1558Google Scholar
  26. 26.
    Li X, Ma S (2015) Advances in the discovery of novel antimicrobials targeting the assembly of bacterial cell division protein FtsZ. Eur J Med Chem 95:1–15Google Scholar
  27. 27.
    Lü J, Sheahan C, Fu P (2011) Metabolic engineering of algae for fourth generation biofuels production. Energ Environ Sci 4(7):2451–2466Google Scholar
  28. 28.
    Mayer AMS, Hamann MT (2005) Marine pharmacology in 2001–2002: marine compounds with anthelmintic, antibacterial, anticoagulant, antidiabetic, antifungal, anti-inflammatory, antimalarial, antiplatelet, antiprotozoal, antituberculosis, and antiviral activities; affecting the cardiovascular, immune and nervous systems and other miscellaneous mechanisms of action. Comp Biochem Phycol 140:265–286Google Scholar
  29. 29.
    Mendes LC, Gazarini ML, Rodrigues (2001) Acclimation of Myrtus communis to contrasting Mediterranean light environments: effects on structure and chemical composition of foliage and plant water relations. Environ Exp Bot 45:165–178Google Scholar
  30. 30.
    Mendiola JA, Herrero M, Cifuentes A, Ibáñez E (2007) Use of compressed fluids for sample preparation: food applications. J Chromatogr 1152:234–246Google Scholar
  31. 31.
    Metting B, Pyne JW (1986) Biologically-active compounds from microalgae. Enzyme Microb Technol 8:386–394Google Scholar
  32. 32.
    Mohamed M, Wei L, Ariff A (2011) Heterotrophic cultivation of microalgae for production of biodiesel. Recent Pat Biotechnol 5:95–107. Google Scholar
  33. 33.
    De Morais MG, Vaz BS, de Morais EG, Costa JAV (2015) Biologically active metabolites synthesized by microalgae. Bio Med Res Int 835761:15Google Scholar
  34. 34.
    Nakamura CV, Nakamura TV, Bando E, Melo AFN, Cortez DAG, Dias FBP (1999) Antibacterial activity of Ocimum gratissimum L. essential oil”. Mem Inst Oswaldo Cruz 94,675–678Google Scholar
  35. 35.
    Nwosu CC, Seipert RR, Strum JS, Hua SS, An HJ, Zivkovic AM, German B, Lebrilla JCB (2011) Simultaneous and extensive site-specific N- and O-glycosylation analysis in protein mixtures. J Proteome Res 10(5):2612–2624Google Scholar
  36. 36.
    Olaizola M (2003) Commercial development of microalgal Biotechnology: from the test tube to the market place. Biomol Eng 20:459–466Google Scholar
  37. 37.
    Olfat MAS, Hoballah EM, Safia MG, Suzy NH (2014) Antimicrobial activity of microalgal extracts with special emphasize on Nostoc sp. Life Sci J 11(12):752–758Google Scholar
  38. 38.
    Oyaizu M (1986) Studies on products of browning reactions: antioxidative activities of products of browning reaction prepared from glucosamine. Jpn J Nutr 44:307–315Google Scholar
  39. 39.
    Patra PK, Takigawa M, Dutton GS, Uhse K, Ishijima K, Lintner BR, Miyazaki K, Elkins JW (2009) Transport mechanisms for synoptic, seasonal and interannual SF6 variations and “age” of air in troposphere. Atmos Chem Phys 9:1209–1225Google Scholar
  40. 40.
    Peng B, Chen X, Shen Y, Bao X (2011) Effect of controlled overexpression of xylulokinase by different promoters on xylose metabolism in Saccharomyces cerevisiae. Acta Microbiol Sin 51(7):914–922Google Scholar
  41. 41.
    Pesando D (1990) Antibacterial and antifungal activities of marine algae. In: Akatsuka I (ed) Introduction to applied phycology. SPB Academic Publishing, The Hague, pp 3–26Google Scholar
  42. 42.
    Philipose MT (1960) Fresh water phytoplankton of inland fisheries. Proc Sym Algal. ICAR, 272–299Google Scholar
  43. 43.
    Powers SK, Jackson MJ (2008) Exercise-induced oxidative stress: cellular mechanisms and impact on muscle force production. Physiol Rev 88(4):1243–1276Google Scholar
  44. 44.
    Prieto P, Pineda M, Anguilar M (1999) Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of Vitamin E. Anal Biochem 269:337–341Google Scholar
  45. 45.
    Pultz O, Gross W (2004) Valuable products from biotechnology of microalgae. Appl Microbiol 65:635–648Google Scholar
  46. 46.
    Samarakoon KW, Ko JY, Lee JH, Kwon ON, Kim SW, Jeon YJ (2014) Apoptotic anticancer activity of a novel fatty alcoholesterisolated from cultured marine diatom, Phaeodactylum tricornutum. J Funct Foods 6:231–240Google Scholar
  47. 47.
    Sanjeet K, Sokona D, Adamou H, Alain R, Dov P, Christophe K (2010) Okra (Abelmoschus spp.) in West and Central Africa: potential and progress on its improvement. Afr J Agric Res 5(25):3590–3598Google Scholar
  48. 48.
    Shakeel AA, Shivasharana CT, Kaliwal BB (2016) Identification and characterisation of Chlorella vulgaris for biodiesel production. Int J Sci Res Eng 3(1):7–15Google Scholar
  49. 49.
    Shettar AK, Vedamurthy AB (2017) An in-vitro approach for evaluating anthelmintic activity of Kandelia candel and Rhizophora apiculata. J Pharmacogn Phytochem 6(1):05–09Google Scholar
  50. 50.
    Singh A, Chaudhary B (2010) Preliminary phycochemical analysis and in vitro antibacterial screening of Pithophora oedogonia (Mont.) Wittrock: a freshwater green alga forming mats in the water bodies. J Algal Biomass Util 1(2):33–41Google Scholar
  51. 51.
    Singleton VL, Orthofer R, Lamuela-Raventos RM (1999) Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol 299:152–178Google Scholar
  52. 52.
    Tiwari A, Thakur N (2016) Nutraceuticals from freshwater microalgae. Int J Ther Appl 32:5–10Google Scholar
  53. 53.
    Uma R, Sivasubramanian V, Devaraj NS (2011) Evaluation of in vitro antioxidant activities and antiproliferative activity of green microalgae, Desmococcus olivaceous and Chlorococcum humicola. J Algal Biomass Utln 2:82–93Google Scholar
  54. 54.
    Valko M, Rhodes CJ, Moncol J (2006) Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chemico-Biol Inter 160:1–40Google Scholar
  55. 55.
    Volk RB, Furkert FH (2005) Antialgal, antibacterial and antifungal activity of two metabolites produced and excreted by cyanobacteria during growth. Microbiol Res 161(2):180–186Google Scholar
  56. 56.
    Wang Y, Xu Z, Bach S, McAllister T (2009) Sensitivity of Escherichia coli to seaweed (Ascophyllum nodosum) phlorotannins and terrestrial tannins. Asian-Australas J Anim Sci 22:238–245Google Scholar
  57. 57.
    Yoo C, Jun S, Lee J, Ahn C, Oh H (2010) Selection of microalgae for lipid production under high levels carbon dioxide. Bioresour Technol 101(1):S71–S74Google Scholar
  58. 58.
    Yun YS, Lee BS, Park TM, Lee C, Yang JW (1997) Carbon dioxide fixation by algal cultivation using waste water nutrients. J Chem Technol Biotechnol 69:451–455Google Scholar
  59. 59.
    Zhang H, Bhattacharya D, Lin S (2005) Phylogeny of dinoflagellates based on mitochondrial cytochrome B and nuclear small subunit rDNA sequence comparison. J Phycol 41:411–420Google Scholar

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© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Studies in Biotechnology and MicrobiologyKarnataka UniversityDharwadIndia

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