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Removal of nitrogen by heterotrophic nitrification–aerobic denitrification of a novel halotolerant bacterium Pseudomonas mendocina TJPU04

  • Xiaoling HeEmail author
  • Qi Sun
  • Tengyao Xu
  • Meng Dai
  • Dongsheng WeiEmail author
Research Paper

Abstract

Excess inorganic nitrogen in water poses a severe threat to enviroment. Removal of inorganic nitrogen by heterotrophic nitrifying–aerobic denitrifying microorganism is supposed to be a promising and applicable technology only if the removal rate can be maintained sufficiently high in real wastewater under various conditions, such as high concentration of salt and wide range of different nitrogen concentrations. Here, a new heterotrophic nitrifying–aerobic denitrifying bacterium was isolated and named as Pseudomonas mendocina TJPU04, which removes NH4+-N, NO3-N and NO2-N with average rate of 4.69, 5.60, 4.99 mg/L/h, respectively. It also maintains high nitrogen removal efficiency over a wide range of nitrogen concentrations. When concentration of NH4+-N, NO3-N and NO2-N was up to 150, 150 and 50 mg/L, 98%, 93%, and 100% removal efficiency could be obtained, respectively, after 30-h incubation under sterile condition. When it was applied under non-sterile condition, the ammonia removal efficiency was slightly lower than that under sterile condition. However, the nitrate and nitrite removal efficiencies under non-sterile condition were significantly higher than those under sterile condition. Strain TJPU04 also showed efficient nitrogen removal performance in the presence of high concentration of salt and nitrogen. In addition, the removal efficiencies of NH4+-N, NO3-N and TN in real wastewater were 91%, 52%, and 75%, respectively. These results suggest that strain TJPU04 is a promising candidate for efficient removal of inorganic nitrogen in wastewater treatment.

Keywords

Pseudomonas mendocina TJPU04 Simultaneous nitrification and denitrification Nitrogen removal Salt tolerance Wastewater treatment 

Notes

Acknowledgements

This work was supported by Natural Science Foundation of Tianjin (no. 16JCYBJC23800), National Science Foundation (no. 31872388), Science and Technology Correspondent Project of Tianjin (no. 18JCTPJC55400) and The Science and Technology Plans of Tianjin (no. 17PTSYJC00040 and 18PTSYJC00180).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. 1.
    Angar Y, Kebbouche-Gana S, Djelali NE, Khemili-Talbi S (2016) Novel approach for the ammonium removal by simultaneous heterotrophic nitrification and denitrification using a novel bacterial species co-culture. World J Microbiol Biotechnol 32:36CrossRefGoogle Scholar
  2. 2.
    Sun Z, Lv Y, Liu Y, Ren R (2016) Removal of nitrogen by heterotrophic nitrification-aerobic denitrification of a novel metal resistant bacterium Cupriavidus sp. S1. Bioresour Technol 220:142–150CrossRefGoogle Scholar
  3. 3.
    Wang JL, Quan XC, Wu LB, Qian Y, Hegemann W (2002) Bioaugmentation as a tool to enhance the removal of refractory compound in coke plant wastewater. Process Biochem 38:777–781CrossRefGoogle Scholar
  4. 4.
    Devlin JF, Eedy R, Butler BJ (2000) The effects of electron donor and granular iron on nitrate transformation rates in sediments from a municipal water supply aquifer. J Contam Hydrol 46:81–97CrossRefGoogle Scholar
  5. 5.
    Tseng IT, Chen JC (2004) The immune response of white shrimp Litopenaeus vannamei and its susceptibility to Vibrio alginolyticus under nitrite stress. Fish Shellfish Immunol 17:325–333CrossRefGoogle Scholar
  6. 6.
    He T, Xie D, Li Z, Ni J, Sun Q (2017) Ammonium stimulates nitrate reduction during simultaneous nitrification and denitrification process by Arthrobacter arilaitensis Y-10. Bioresour Technol 239:66–73CrossRefGoogle Scholar
  7. 7.
    Chen P, Li J, Li QX, Wang Y, Li S, Ren T, Wang L (2012) Simultaneous heterotrophic nitrification and aerobic denitrification by bacterium Rhodococcus sp. CPZ24. Bioresour Technol 116:266–270CrossRefGoogle Scholar
  8. 8.
    Zhao L, She Z, Jin C, Yang S, Guo L, Zhao Y, Gao M (2016) Characteristics of extracellular polymeric substances from sludge and biofilm in a simultaneous nitrification and denitrification system under high salinity stress. Bioprocess Biosyst Eng 39:1375–1389CrossRefGoogle Scholar
  9. 9.
    Li H, Pan M, Zhou S, Huang S, Zhang Y10 (2016) Characterization of nitrous oxide emissions from a thermophilic denitrifying bacterium Chelatococcus daeguensis TAD1 in an aerated sequencing batch reactor. Biochem Eng J 112:233–240CrossRefGoogle Scholar
  10. 10.
    Zhou Y (2010) Using sludge fermentation liquid to improve wastewater short-cut nitrification–denitrification and denitrifying phosphorus removal via nitrite. Environ Sci Technol 44(23):8957–8963CrossRefGoogle Scholar
  11. 11.
    Ji B, Yang K, Wang H, Zhou J, Zhang H (2015) Aerobic denitrification by Acinetobacter sp. WB-1 during simultaneous nitrification/denitrification process. J Chem Technol Biotechnol 92:649–656Google Scholar
  12. 12.
    Robertson LA, Kuenen JG (1990) Combined heterotrophic nitrification and aerobic denitrification in Thiosphaera pantotropha and other bacteria. Antonie Van Leeuwenhoek Int J Gen 57:139–152CrossRefGoogle Scholar
  13. 13.
    Zhang QL, Liu Y, Ai GM, Miao LL, Zheng HY, Liu ZP (2012) The characteristics of a novel heterotrophic nitrification–aerobic denitrification bacterium, Bacillus methylotrophicus strain L7. Bioresour Technol 108:35–44CrossRefGoogle Scholar
  14. 14.
    Jin R, Liu T, Liu G, Zhou J, Huang J, Wang A (2015) Simultaneous heterotrophic nitrification and aerobic denitrification by the marine origin bacterium Pseudomonas sp. ADN-42. Appl Biochem Biotechnol 175:2000–2011CrossRefGoogle Scholar
  15. 15.
    Huang TL, Zhou SL, Zhang HH, Zhou N, Guo L, Di SY, Zhou ZZ (2015) Nitrogen removal from micro-polluted reservoir water by indigenous aerobic denitrifiers. Int J Mol Sci 16:8008–8026CrossRefGoogle Scholar
  16. 16.
    Shoda M, Ishikawa Y (2014) Heterotrophic nitrification and aerobic denitrification of high-strength ammonium in anaerobically digested sludge by Alcaligenes faecalis strain no. 4. J Biosci Bioeng 117:737–741CrossRefGoogle Scholar
  17. 17.
    Padhi SK, Tripathy S, Sen R, Mahapatra AS, Mohanty S, Maiti NK (2013) Characterisation of heterotrophic nitrifying and aerobic denitrifying Klebsiella pneumoniae CF-S9 strain for bioremediation of wastewater. Int Biodeterior Biodegrad 78:67–73CrossRefGoogle Scholar
  18. 18.
    Wang Y, Zhang Z, Qiu L, Guo Y, Wang X, Xiong X, Chen S (2015) Effect of temperature downshifts on biological nitrogen removal and community structure of a lab-scale aerobic denitrification process. Biochem Eng J 101:200–208CrossRefGoogle Scholar
  19. 19.
    Chen J, Zhao B, An Q, Wang X, Zhang YX (2016) Kinetic characteristics and modelling of growth and substrate removal by Alcaligenes faecalis strain NR. Bioprocess Biosyst Eng 39:593–601CrossRefGoogle Scholar
  20. 20.
    Gui M, Chen Q, Ni J (2017) Effect of NaCl on aerobic denitrification by strain Achromobacter sp. GAD-3. Appl Microbiol Biotechnol 101:5139–5147CrossRefGoogle Scholar
  21. 21.
    Joo HS, Hirai M, Shoda M (2007) Improvement in ammonium removal efficiency in wastewater treatment by mixed culture of Alcaligenes faecalis no. 4 and L1. J Biosci Bioeng 103:66–73CrossRefGoogle Scholar
  22. 22.
    Rout PR, Bhunia P, Dash RR (2017) Simultaneous removal of nitrogen and phosphorous from domestic wastewater using Bacillus cereus GS-5 strain exhibiting heterotrophic nitrification, aerobic denitrification and denitrifying phosphorous removal. Bioresour Technol 244:484–495CrossRefGoogle Scholar
  23. 23.
    APHA (1998) Standard methods for the examination of water and wastewater, 20th edn. American Public Health Association, Washington, DCGoogle Scholar
  24. 24.
    Wan W, He D, Xue Z (2017) Removal of nitrogen and phosphorus by heterotrophic nitrification-aerobic denitrification of a denitrifying phosphorus-accumulating bacterium Enterobacter cloacae HW-15. Ecol Eng 99:199–208CrossRefGoogle Scholar
  25. 25.
    Tian L, Ma P, Zhong JJ (2002) Kinetics and key enzyme activities of phenanthrene degradation by Pseudomonas mendocina. Process Biochem 37:1431–1437CrossRefGoogle Scholar
  26. 26.
    Miranda-Carrazco A, Vigueras-Cortés JM, Villa-Tanaca L, Hernández-Rodríguez C (2018) Cyanotrophic and arsenic oxidizing activities of Pseudomonas mendocina P6115 isolated from mine tailings containing high cyanide concentration. Arch Microbiol 200:1037–1048CrossRefGoogle Scholar
  27. 27.
    Zhang S, Sun X (2017) Heterotrophic nitrification and aerobic denitrification by Diaphorobacter polyhydroxybutyrativorans SL-205 using poly(3-hydroxybutyrate-co-3-hydroxyvalerate) as the sole carbon source. Bioresour Technol 241:500–507CrossRefGoogle Scholar
  28. 28.
    Ren YX, Yang L, Liang X (2014) The characteristics of a novel heterotrophic nitrifying and aerobic denitrifying bacterium, Acinetobacter junii YB. Bioresour Technol 171:1–9CrossRefGoogle Scholar
  29. 29.
    Sun Y, Li A, Zhang X, Ma F (2015) Regulation of dissolved oxygen from accumulated nitrite during the heterotrophic nitrification and aerobic denitrification of Pseudomonas stutzeri T13. Appl Microbiol Biotechnol 99:3243–3248CrossRefGoogle Scholar
  30. 30.
    Ye Q, Li K, Li Z, Xu Y, He T, Tang W, Xiang S (2017) Heterotrophic nitrification–aerobic denitrification performance of strain Y-12 under low temperature and high concentration of inorganic nitrogen conditions. Water 9:835CrossRefGoogle Scholar
  31. 31.
    Huang X, Li W, Zhang D, Qin W (2013) Ammonium removal by a novel oligotrophic Acinetobacter sp. Y16 capable of heterotrophic nitrification–aerobic denitrification at low temperature. Bioresour Technol 146:44–50CrossRefGoogle Scholar
  32. 32.
    He T, Li Z, Sun Q, Xu Y, Ye Q (2016) Heterotrophic nitrification and aerobic denitrification by Pseudomonas tolaasii Y-11 without nitrite accumulation during nitrogen conversion. Bioresour Technol 200:493–499CrossRefGoogle Scholar
  33. 33.
    Huang G, Ou L, Pan F, Wang Y, Fan G, Liu G, Wang W (2017) Isolation of a novel heterotrophic nitrification–aerobic denitrification bacterium Serratia marcescens CL1502 from deep-sea sediment. Environ Eng Sci 34:453–459CrossRefGoogle Scholar
  34. 34.
    Li C, Yang J, Wang X, Wang E, Li B, He R, Yuan H (2015) Removal of nitrogen by heterotrophic nitrification–aerobic denitrification of a phosphate accumulating bacterium Pseudomonas stutzeri YG-24. Bioresour Technol 182:18–25CrossRefGoogle Scholar
  35. 35.
    Yang Y, Liu Y, Yang T, Lv Y (2017) Characterization of a microbial consortium capable of heterotrophic nitrifying under wide C/N range and its potential application in phenolic and coking wastewater. Biochem Eng J 120:33–40CrossRefGoogle Scholar
  36. 36.
    Zhao B, He YL, Zhang XF (2010) Nitrogen removal capability through simultaneous heterotrophic nitrification and aerobic denitrification by Bacillus sp. LY. Environ Technol 31:409–416CrossRefGoogle Scholar
  37. 37.
    Zhang Y, Shi Z, Chen M, Dong X, Zhou J (2015) Evaluation of simultaneous nitrification and denitrification under controlled conditions by an aerobic denitrifier culture. Bioresour Technol 175:602–605CrossRefGoogle Scholar
  38. 38.
    Huang F, Pan L, Lv N, Tang X (2017) Characterization of novel Bacillus strain N31 from mariculture water capable of halophilic heterotrophic nitrification–aerobic denitrification. J Biosci Bioeng 124:564–571CrossRefGoogle Scholar
  39. 39.
    Yang X, Wang S, Zhou L (2012) Effect of carbon source, C/N ratio, nitrate and dissolved oxygen concentration on nitrite and ammonium production from denitrification process by Bacillus strain N31 from mariculture water capable of halophilic heterotrophic nitrification–aerobic denitrification. J Biosci Bioeng 124:564–571Google Scholar
  40. 40.
    Joo HS, Hirai M, Shoda M (2005) Characteristics of ammonium removal by heterotrophic nitrification–aerobic denitrification by Alcaligenes faecalis no. 4. J Biosci Bioeng 100:184–191CrossRefGoogle Scholar
  41. 41.
    Chen J, Gu S, Hao H, Chen J (2016) Characteristics and metabolic pathway of Alcaligenes sp. TB for simultaneous heterotrophic nitrification–aerobic denitrification. Appl Microbiol Biotechnol 100:9787–9794CrossRefGoogle Scholar
  42. 42.
    Qu D, Wang C, Wang Y, Zhou R, Ren H (2015) Heterotrophic nitrification and aerobic denitrification by a novel groundwater origin cold-adapted bacterium at low temperatures. RSC Adv 5:5149–5157CrossRefGoogle Scholar
  43. 43.
    Guo Y, Zhou X, Li Y, Li K, Wang C, Liu J, Yan D, Liu Y, Yang D, Xing J (2013) Heterotrophic nitrification and aerobic denitrification by a novel Halomonas campisalis. Biotechnol Lett 35:2045–2049CrossRefGoogle Scholar
  44. 44.
    Duan J, Fang H, Su B, Chen J, Lin J (2015) Characterization of a halophilic heterotrophic nitrification–aerobic denitrification bacterium and its application on treatment of saline wastewater. Bioresour Technol 179:421–428CrossRefGoogle Scholar
  45. 45.
    Lu Y, Feng L, Yang G, Yang Q, Zhang X, Mu J (2018) Intensification and microbial pathways of simultaneous nitrification–denitrification in a sequencing batch biofilm reactor for seawater-based saline wastewater treatment. J Chem Technol Biotechnol 93:2766–2773CrossRefGoogle Scholar
  46. 46.
    Patureau D, Zumstein E, Delgenes JP, Moletta R (2000) Aerobic denitrifiers isolated from diverse natural and managed ecosystems. Microb Ecol 39:145–152CrossRefGoogle Scholar
  47. 47.
    Zhang J, Wu P, Hao B, Yu Z (2011) Heterotrophic nitrification and aerobic denitrification by the bacterium Pseudomonas stutzeri YZN-001. Bioresour Technol 102:9866–9869CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.State Key Laboratory of Separation Membranes and Membrane Process, National Center for International Joint Research on Separation Membranes, School of Chemistry and Chemical EngineeringTianjin Polytechnic UniversityTianjinChina
  2. 2.School of Environmental Science and EngineeringTianjin Polytechnic UniversityTianjinChina
  3. 3.Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life ScienceNankai UniversityTianjinChina

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