Strategies related to light quality and temperature to improve lutein production of marine microalga Chlamydomonas sp.

  • Xurui Zhao
  • Ruijuan Ma
  • Xiaoting Liu
  • Shih-Hsin Ho
  • Youping XieEmail author
  • Jianfeng ChenEmail author
Research Paper


The marine microalga Chlamydomonas sp. JSC4 was examined for its potential as a lutein producer. Environmental conditions, including light quality, temperature and light wavelength mixing ratio, were individually altered to enhance the cell growth rate and lutein production in strain JSC4. Results showed that optimal cell growth was obtained under white light and a temperature of 35 °C, while the optimal lutein content was obtained under blue light and a lower temperature of 20–25 °C. The best lutein production occurred when using a mixing ratio of 3:1 (white light: blue light). Strategies related to light quality and temperature (namely, temperature-gradient and two-stage strategies) were then used to further improve lutein production. Among them, the two-stage strategy proved to be effective markedly improving lutein content from 2.52 to 4.24 mg/g and resulting in the highest lutein productivity of 3.25 mg/L/day.


Microalgae Lutein Light quality Temperature Two-stage strategy 



This work was supported by the National Natural Science Foundation of China (No. 41641050), the Natural Science Foundation of Fujian Province, China (No. 2016J05077), the Oceans and Fisheries Bureau of Fuzhou, China (No. FZHJ15 and FZHJ04), and the Special Fund for Fujian Ocean High-Tech Industry Development, China (No. [2016] 15).


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.College of Biological Science and EngineeringFuzhou UniversityFuzhouChina
  2. 2.Fujian Engineering and Technology Research Center for Comprehensive Utilization of Marine Products WasteFuzhou UniversityFuzhouChina
  3. 3.State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental EngineeringHarbin Institute of TechnologyHarbinChina

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