Acetone, butanol, and ethanol production from the green seaweed Enteromorpha intestinalis via the separate hydrolysis and fermentation

  • Trung Hau Nguyen
  • In Yung Sunwoo
  • Chae Hun Ra
  • Gwi-Taek Jeong
  • Sung-Koo Kim
Research Paper


Acetone, butanol, and ethanol (ABE) were produced following the separate hydrolysis and fermentation (SHF) method using polysaccharides from the green macroalgae Enteromorpha intestinalis as biomass. We focused on the optimization of enzymatic saccharification as pretreatments for the fermentation of E. intestinalis. Pretreatment was carried out with 10% (w/v) seaweed slurry and 270-mM H2SO4 at 121 °C for 60 min. Monosaccharides (mainly glucose) were obtained from enzymatic hydrolysis with a 16-U/mL mixture of Celluclast 1.5 L and Viscozyme L at 45 °C for 36 h. ABE fermentation with 10% (w/v) E. intestinalis hydrolysate was performed using the anaerobic bacteria Clostridium acetobutylicum with either uncontrolled pH, pH controlled at 6.0, or pH controlled initially at 6.0 and then 4.5 after 4 days, which produced ABE contents of 5.6 g/L with an ABE yield (YABE) of 0.24 g/g, 4.8 g/L with an YABE of 0.2 g/g, and 8.5 g/L with an YABE of 0.36 g/g, respectively.


Clostridium acetobutylicum Enteromorpha intestinalis Enzymatic saccharification Fermentation Thermal acid hydrolysis 



This research was supported by Basic Science Research Program through the National Research Foundation of Korea, funded by the Ministry of Education (2016R1D1A1A09918683).

Compliance with ethical standards

Conflict of interest

The authors indicate no potential conflicts of interest.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Trung Hau Nguyen
    • 1
  • In Yung Sunwoo
    • 1
  • Chae Hun Ra
    • 2
  • Gwi-Taek Jeong
    • 1
  • Sung-Koo Kim
    • 1
  1. 1.Department of BiotechnologyPukyong National UniversityBusanRepublic of Korea
  2. 2.Department of Food Science and BiotechnologyHankyong National UniversityAnseongRepublic of Korea

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