Potential of in situ SSF laccase produced from Ganoderma lucidum RCK 2011 in biobleaching of paper pulp

  • Abha Sharma
  • Kavish Kumar Jain
  • Anita Srivastava
  • Bhuvnesh Shrivastava
  • Vasanta Vadde Thakur
  • R. K. Jain
  • R. C. Kuhad
Research Paper


Production of laccase from Ganoderma lucidum RCK 2011 under solid-state fermentation (SSF) conditions was optimized using response surface methodology, resulting in an approximate eightfold increase compared to that in the unoptimized media. Further, the enzyme produced under SSF as whole fermented substrate (in situ SSF laccase) was found to be more stable than the in vitro enzyme (harvested by downstreaming processing of fermented wheat bran). Interestingly, the biobleaching potentials of both in situ and in vitro SSF laccases were comparable, saving 25% chlorine dioxide for achieving similar pulp brightness as obtained in the pulp treated chemically. The reduction in the demand of chlorine dioxide in the pulp bleaching sequence subsequently decreased the levels of adsorbable organic halogen (AOX) in the resulting effluents of the process by 20% compared to the effluents obtained from chemical bleaching sequence. Therefore, direct application of in situ SSF laccase in pulp biobleaching will be environmentally friendly as well as economical and viable for implementation in paper mills.


Solid-state fermentation (SSF) Laccase Biobleaching Eucalyptus kraft pulp AOX 



Financial assistance procured from the Department of Biotechnology (DBT), Ministry of Science and Technology, Govt. of India is acknowledged by all the authors. The necessary research facilities provided by the University of Delhi, South Campus, and CPPRI, Saharanpur, U.P., is highly acknowledged by all the authors of the manuscript. AS acknowledges the Department of Science and Technology, India, for the Women Scientist Fellowship (File no. SR/WOS-A/LS-700/2016). KKJ acknowledges the Science and Engineering Research Board, India, for the postdoctoral fellowship (File no. PDF/2016/001068). BS wishes to acknowledge the DST Fast Track Young Scientist Grant (File no. YSS/2015/001950) for the support during the current study.

Compliance with ethical standards

Conflict of interest

The authors do not have any conflict of interest.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Abha Sharma
    • 1
  • Kavish Kumar Jain
    • 1
    • 4
  • Anita Srivastava
    • 1
  • Bhuvnesh Shrivastava
    • 1
    • 5
  • Vasanta Vadde Thakur
    • 2
  • R. K. Jain
    • 2
  • R. C. Kuhad
    • 1
    • 3
  1. 1.Lignocellulose Biotechnology Laboratory, Department of MicrobiologyUniversity of DelhiNew DelhiIndia
  2. 2.Central Paper and Pulp Research InstituteSaharanpurIndia
  3. 3.Central University of HaryanaMahendargarhIndia
  4. 4.Department of MicrobiologyMahrashi Dayanand UniversityRohtakIndia
  5. 5.One Stream Research CentrePanacea Biotec Ltd.New DelhiIndia

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