Influence of amino acids and vitamins on the growth of gdhA derivative Pasteurella multocida B:2 for use as an animal vaccine
Pasteurella multocida serotype B:2 is the causative agent of haemorrhagic septicaemia, a fatal disease in cattle and buffaloes. For use as a vaccine in the treatment of HS disease, an efficient cultivation of attenuated gdhA derivative P. multocida B:2 (mutant) for mass production of viable cells is required. In this study, the role of amino acids and vitamins on the growth of this particular bacterium was investigated. Initially, three basal media (Brain–heart infusion, Terrific broth, and defined medium YDB) were assessed in terms of growth performance of P. multocida B:2. YDB medium was selected and redesigned to take into account the effects of amino acids (glutamic acid, cysteine, glycine, methionine, lysine, tyrosine, and histidine) and vitamins (vitamin B1, nicotinic acid, riboflavin, pyridoxine, pantothenic acid, and biotin). High viable cell number was largely affected by the availability of micronutrient components and macronutrients. Histidine was essential for the growth whereby a traceable amount (20 mM) was found to greatly enhance the growth of gdhA derivative P. multocida B:2 mutant (6.6 × 109 cfu/mL) by about 19 times as compared to control culture (3.5 × 108 cfu/mL). In addition, amongst the vitamins added, riboflavin exhibited the highest impact on the viability of gdhA derivative P. multocida B:2 mutant (5.3 × 109 cfu/mL). Though the combined histidine and riboflavin in the culture eventually did not promote the stacking impact on cell growth and cell viability, nonetheless, they were still essential and important in either growth medium or production medium.
KeywordsGdhA derivative Pasteurella multocida B:2 Cell viability Amino acid Vitamins
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Conflicts of interest
The authors declare that they have no conflict of interest.
Research involving human participants and/or animals
This article does not contain any studies with human participants or animals performed by any of the authors.
- 1.Abdullah FFJ, Adamu L, Osman AY, Zakaria Z, Abdullah R, Saad MZ, Saharee AA (2013) Clinico-pathological responses of calves associated with infection of” Pasteurella multocida” Type B and the bacterial lipopolysaccharide and outer membrane protein immunogens. Int J Anim Vet Adv 5:190–198Google Scholar
- 2.Prasannavadhana A, Kumar S, Thomas P, Sarangi LN, Gupta SK, Priyadarshini A, Nagaleekar VK, Singh VP (2014) Outer membrane proteome analysis of indian strain of Pasteurella multocida serotype B: 2 by MALDI-TOF/MS analysis. Sci World JGoogle Scholar
- 3.Tabatabaei M, Jula GM, Jabbari A, Esmailzadeh M (2007) Vaccine efficacy in cattle against hemorrhagic septicemia with live attenuated aroA mutant of Pasteurella multocida B: 2 strain. J Cell Anim Biol 1:062–065Google Scholar
- 4.Oslan SNH (2013) In vivo survivality and optimization of parameters for biomass production of GDHA derivative of Pasteurella multocida B: 2. Universiti Putra MalaysiaGoogle Scholar
- 6.Othman SS (2007) Construction of an attenuated Pasteurella multocida B: 2 by mutation in the gdha gene. Universiti Putra MalaysiaGoogle Scholar
- 13.Sarah S, Zamri-Saad M, Zunita Z, Raha A (2006) Molecular cloning and sequence analysis of gdhA gene of Pasteurella multocida B: 2. J Anim Vet Adv 5:1146–1149Google Scholar
- 17.Shah A, Kamboh A, Rajput N, Korejo N (2008) Optimization of physico-chemical conditions for the growth of Pasteurella multocida in vitro. J Agric Soc SciGoogle Scholar
- 20.Fernández A, Saguir F, Manca de Nadra M (2003) Effect of amino acids and peptides on growth of Pediococcus pentosaceus from wine. Lat Am Appl Res 33:225–229Google Scholar
- 25.Magasanik B (1992) Regulation of nitrogen utilization. Cold Spring Harbor Monogr Arch 21:283–317Google Scholar
- 28.Kosbb S (1968) Vitamin requirements of bacteria and yeasts. Vitamin requirements of bacteria and yeasts. ThomasGoogle Scholar