Bioprocess and Biosystems Engineering

, Volume 42, Issue 2, pp 173–186 | Cite as

Emerging techniques for cell disruption and extraction of valuable bio-molecules of microalgae Nannochloropsis sp.

  • Rui ZhangEmail author
  • Oleksii Parniakov
  • Nabil Grimi
  • Nikolai Lebovka
  • Luc Marchal
  • Eugène Vorobiev
Critical Review


Microalgae of Nannochloropsis sp. present valuable source of bio-molecules (pigments, lipids, proteins) that have nutritional potential for the prevention and treatment of human diseases. Moreover, some species of Nannochloropsis are the promising sources of biofuels and excellent candidates for the replacement of classical biofuel crops. This review describes and compares the efficiency of different conventional and novel techniques that can be used for cell disruption and recovery of bio-molecules from Nannochloropsis sp. Classification of different extraction techniques includes chemical, enzymatic, mechanical and other physical methods. The detailed analysis of extraction efficiency assisted by pressure and temperature (subcritical and supercritical fluids, hydrothermal liquefaction), ultrasound, microwaves, and pulsed electric energy (pulsed electric fields and high voltage electrical discharges) is presented. The general discussion includes comparison between techniques, their effectiveness for cell disruption and selectivity of bio-molecules extraction from Nannochloropsis sp. The cost-effectiveness, benefits and limitations of different techniques are also analyzed.

Graphical abstract


Microalgae Nannochloropsis sp. Extraction Bio-molecules Disruption techniques 



Rui Zhang would like to acknowledge the financial support of China Scholarship Council for thesis fellowship.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

449_2018_2038_MOESM1_ESM.doc (157 kb)
Supplementary material 1 (DOC 157 KB)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Rui Zhang
    • 1
    Email author
  • Oleksii Parniakov
    • 1
  • Nabil Grimi
    • 1
  • Nikolai Lebovka
    • 1
    • 2
  • Luc Marchal
    • 3
  • Eugène Vorobiev
    • 1
  1. 1.Laboratoire de Transformations Intégrées de la Matière Renouvelable, EA 4297, Centre de Recherches de RoyallieuSorbonne Universités, Université de Technologie de CompiègneCompiègne CedexFrance
  2. 2.Institute of Biocolloidal Chemistry named after F. D. OvcharenkoNAS of UkraineKyivUkraine
  3. 3.LUNAM Université, CNRS, GEPEA, Université de Nantes, UMR6144, CRTT, Boulevard de l’UniversitéSaint-Nazaire CedexFrance

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