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Outdoor microalgae cultivation in airlift photobioreactor at high irradiance and temperature conditions: effect of batch and fed-batch strategies, photoinhibition, and temperature stress

  • Suvidha Gupta
  • Sanjay B. PawarEmail author
  • R. A. Pandey
  • Gajanan S. Kanade
  • Satish K. Lokhande
Research Paper
  • 42 Downloads

Abstract

The microalgae Scenedesmus abundans cultivated in five identical airlift photobioreactors (PBRs) in batch and fed-batch modes at the outdoor tropical condition. The microalgae strain S. abundans was found to tolerate high temperature (35–45 °C) and high light intensity (770–1690 µmol m− 2 s− 1). The highest biomass productivities were 152.5–162.5 mg L− 1 day− 1 for fed-batch strategy. The biomass productivity was drastically reduced due to photoinhibition effect at a culture temperature of > 45 °C. The lipid compositions showed fatty acids mainly in the form of saturated and monounsaturated fatty acids (> 80%) in all PBRs with Cetane number more than 51. The fed-batch strategies efficiently produced higher biomass and lipid productivities at harsh outdoor conditions. Furthermore, the microalgae also accumulated omega-3 fatty acid (C18:3) up to 14% (w/w) of total fatty acid at given outdoor condition.

Keywords

Dark respiration Biodiesel Mixotrophic cultivation Airlift photobioreactor Fed-batch strategy 

Notes

Acknowledgements

The corresponding author is thankful to the Department of Science and Technology, New Delhi, India for their financial support for this research work under the scheme of DST INSPIRE Faculty Award (IFA13–ENG63). Ms. Suvidha Gupta is thankful to Council of Scientific and Industrial Research (CSIR), New Delhi, India for providing Senior Research Fellowship (SRF) to her for Ph.D. research work.

Supplementary material

449_2018_2037_MOESM1_ESM.doc (586 kb)
Supplementary material 1 (DOC 585 KB)

References

  1. 1.
    Hindersin S, Leupold M, Kerner M, Hanelt D (2013) Irradiance optimization of outdoor microalgal cultures using solar tracked photobioreactors. Bioprocess Biosyst Eng 36:345–355CrossRefGoogle Scholar
  2. 2.
    Yoo JJ, Choi SP, Kim JY, Chang WS, Sim SJ (2013) Development of thin-film photo-bioreactor and its application to outdoor culture of microalgae. Bioprocess Biosyst Eng 36:729–736CrossRefGoogle Scholar
  3. 3.
    Pawar S (2016) Effectiveness mapping of open raceway pond and tubular photobioreactors for sustainable production of microalgae biofuel. Renew Sustain Energy Rev 62:640–653CrossRefGoogle Scholar
  4. 4.
    Koller AP, Wolf L, Brück T, Weuster-Botz D (2018) Studies on the scale-up of biomass production with Scenedesmus spp. in flat-plate gas-lift photobioreactors. Bioprocess Biosyst Eng 41:213–220CrossRefGoogle Scholar
  5. 5.
    Chiu PH, Soong K, Chen CNN (2016) Cultivation of two thermotolerant microalgae under tropical conditions: influences of carbon sources and light duration on biomass and lutein productivity in four seasons. Bioresour Technol 212:190–198CrossRefGoogle Scholar
  6. 6.
    Xin L, Hong-ying H, Yu-ping Z (2011) Growth and lipid accumulation properties of a freshwater microalga Scenedesmus sp. under different cultivation temperature. Bioresour Technol 102:3098–3102CrossRefGoogle Scholar
  7. 7.
    Barati B, Lim PE, Gan SY, Poong SW, Phang SM, Beardall J (2018) Effect of elevated temperature on the physiological responses of marine Chlorella strains from different latitudes. J Appl Phycol 30:1–13CrossRefGoogle Scholar
  8. 8.
    Nishiyama Y, Allakhverdiev SI, Murata N (2006) A new paradigm for the action of reactive oxygen species in the photoinhibition of photosystem II. Biochim Biophys Acta 1757:742–749CrossRefGoogle Scholar
  9. 9.
    Murata N, Takahashi S, Nishiyama Y, Allakhverdiev SI (2007) Photoinhibition of photosystem II under environmental stress. Biochim Biophys Acta 1767:414–421CrossRefGoogle Scholar
  10. 10.
    Hoekman SK, Broch A, Robbins C, Ceniceros E, Natarajan M (2012) Review of biodiesel composition, properties, and specifications. Renew Sustain Energy Rev 16:143–169CrossRefGoogle Scholar
  11. 11.
    Ceron-Garcia MC, Fernandez-Sevilla JM, Sanchez-Miron A, Garcia-Camacho F, Contreras-Gomez A, Molina-Grima E (2013) Mixotrophic growth of Phaeodactylum tricornutum on fructose and glycerol in fed-batch and semi-continuous modes. Bioresour Technol 147:569–576CrossRefGoogle Scholar
  12. 12.
    Moon M, Kim CW, Park WK, Yoo G, Choi YE, Yang JW (2013) Mixotrophic growth with acetate or volatile fatty acids maximizes growth and lipid production in Chlamydomonas reinhardtii. Algal Res 2:352–357CrossRefGoogle Scholar
  13. 13.
    Cheirsilp B, Torpee S (2012) Enhanced growth and lipid production of microalgae under mixotrophic culture condition: Effect of light intensity, glucose concentration and fed-batch cultivation. Bioresour Technol 110:510–516CrossRefGoogle Scholar
  14. 14.
    Bouarab L, Dauta A, Loudiki M (2004) Heterotrophic and mixotrophic growth of Micractinium pusillum Fresenius in the presence of acetate and glucose: effect of light and acetate gradient concentration. Water Res 38:2706–2712CrossRefGoogle Scholar
  15. 15.
    Miron AS, Garcia MCC, Camacho FG, Grima EM, Chisti Y (2002) Growth and biochemical characterization of microalgal biomass produced in bubble column and airlift photobioreactors: studies in fed-batch culture. Enzyme Microb Technol 31:1015–1023CrossRefGoogle Scholar
  16. 16.
    Najafabadi HA, Malekzadeh M, Jalilian F, Vossoughi M, Pazuki G (2015) Effect of various carbon sources on biomass and lipid production of Chlorella vulgaris during nutrient sufficient and nitrogen starvation conditions. Bioresour Technol 180:311–317CrossRefGoogle Scholar
  17. 17.
    Fei Q, Fu R, Shang L, Brigham CJ, Chang HN (2015) Lipid production by microalgae Chlorella protothecoides with volatile fatty acids (VFAs) as carbon sources in heterotrophic cultivation and its economic assessment. Bioprocess Biosyst Eng 38:691–700CrossRefGoogle Scholar
  18. 18.
    Gupta S, Pawar SB (2018) An integrated approach for microalgae cultivation using raw and anaerobic digested wastewaters from food processing industry. Bioresour Technol 269:571–576CrossRefGoogle Scholar
  19. 19.
    Liu X, Duan S, Li A, Xu N, Cai Z, Hu Z (2009) Effects of organic carbon sources on growth, photosynthesis, and respiration of Phaeodactylum tricornutum. J Appl Phycol 21:239–246CrossRefGoogle Scholar
  20. 20.
    Pawar SB (2016) Process engineering aspects of vertical gas sparged photobioreactors for mass production of microalgae. Chem Bio Eng Rev 3:101–115Google Scholar
  21. 21.
    Negi S, Barry AN, Friedland N, Sudasinghe N, Subramanian S, Pieris S, Holguin O, Dungan B, Schaub T, Sayre R (2016) Impact of nitrogen limitation on biomass, photosynthesis, and lipid accumulation in Chlorella sorokiniana. J Appl Phycol 28:803–812CrossRefGoogle Scholar
  22. 22.
    Lichtenthaler HK (1987) Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. In: Packer L, Douce R (eds) Methods in enzymology. Academic Press, London, pp 350–382Google Scholar
  23. 23.
    Anahas AMP, Muralitharan G (2015) Isolation and screening of heterocystous cyanobacterial strains for biodiesel production by evaluating the fuel properties from fatty acid methyl ester (FAME) profiles. Bioresour Technol 184:9–17CrossRefGoogle Scholar
  24. 24.
    Gupta S, Pawar SB (2018) Mixotrophic cultivation of microalgae to enhance the quality of lipid for biodiesel application: effects of scale of cultivation and light spectrum on reduction of α-linolenic acid. Bioprocess Biosyst Eng 41:531–542CrossRefGoogle Scholar
  25. 25.
    Krisnangkura KA (1986) Simple method for estimation of cetane index of vegetable oil methyl esters. J Am Oil Chem Soc 63:552–553CrossRefGoogle Scholar
  26. 26.
    Ong SC, Kao CY, Chiu SY, Tsai MT, Lin CS (2010) Characterization of the thermal-tolerant mutants of Chlorella sp. with high growth rate and application in outdoor photobioreactor cultivation. Bioresour Technol 101:2880–2883CrossRefGoogle Scholar
  27. 27.
    Chu HQ, Tan XB, Zhang YL, Yang LB, Zhao FC, Guo J (2015) Continuous cultivation of Chlorella pyrenoidosa using anaerobic digested starch processing wastewater in the outdoors. Bioresour Technol 185:40–48CrossRefGoogle Scholar
  28. 28.
    Tan X, Chu H, Zhang Y, Yang L, Zhao F, Zhou X (2014) Chlorella pyrenoidosa cultivation using anaerobic digested starch processing wastewater in an airlift circulation photobioreactor. Bioresour Technol 170:538–548CrossRefGoogle Scholar
  29. 29.
    Wang SK, Hu YR, Wang F, Stiles AR, Liu CZ (2014) Scale-up cultivation of Chlorella ellipsoidea from indoor to outdoor in bubble column bioreactors. Bioresour Technol 156:117–122CrossRefGoogle Scholar
  30. 30.
    Guo Z, Phooi WBA, Lim ZJ, Tong YW (2015) Control of CO2 input conditions during outdoor culture of Chlorella vulgaris in bubble column photobioreactors. Bioresour Technol 186:238–245CrossRefGoogle Scholar
  31. 31.
    Huang CC, Hung JJ, Peng SH, Chen CNN (2012) Cultivation of a thermo-tolerant microalga in an outdoor photobioreactor: Influences of CO2 and nitrogen sources on the accelerated growth. Bioresour Technol 112:228–233CrossRefGoogle Scholar
  32. 32.
    Lu W, Wang Z, Wang X, Yuan Z (2015) Cultivation of Chlorella sp. using raw dairy wastewater for nutrient removal and biodiesel production: characteristics comparison of indoor bench-scale and outdoor pilot-scale cultures. Bioresour Technol 192:382–388CrossRefGoogle Scholar
  33. 33.
    Wagenen JV, Francisci DD, Angelidaki I (2015) Comparison of mixotrophic to cyclic autotrophic/heterotrophic growth strategies to optimize productivity of Chlorella sorokiniana. J Appl Phycol 27:1775–1782CrossRefGoogle Scholar
  34. 34.
    Gupta S, Pandey R, Pawar S (2017) Bioremediation of synthetic high strength chemical oxygen demand wastewater using microalgae sp. Chlorella pyrenoidosa. Bioremediat J 21:38–51CrossRefGoogle Scholar
  35. 35.
    Edmundson SJ, Huesemann MH (2015) The dark side of algae cultivation: characterizing night biomass loss in three photosynthetic algae, Chlorella sorokiniana, Nannochloropsis salina and Picochlorum sp. Algal Res 12:470–476CrossRefGoogle Scholar
  36. 36.
    Krzeminiska I, Oleszek M (2016) Glucose supplementation-induced changes in the Auxenochlorella protothecoides fatty acid composition suitable for biodiesel production. Bioresour Technol 218:1294–1297CrossRefGoogle Scholar
  37. 37.
    Yang L, Chen J, Qin S, Zeng M, Jiang Y, Hu L, Xiao P, Hao W, Hu Z, Lei A, Wang J (2018) Growth and lipid accumulation by different nutrients in the microalga Chlamydomonas reinhardtii. Biotechnol Biofuels 11:40CrossRefGoogle Scholar
  38. 38.
    Li T, Zheng Y, Yu L, Chen S (2014) Mixotrophic cultivation of a Chlorella sorokiniana strain for enhanced biomass and lipid production. Biomass Bioenerg 66:204–213CrossRefGoogle Scholar
  39. 39.
    Chen F, Johns MR (1991) Effect of C/N ratio and aeration on the fatty acid composition of heterotrophic Chlorella sorokiniana. J Appl Phycol 3:203–209CrossRefGoogle Scholar
  40. 40.
    Pawar S, Gupta S (2018) Mass production of microalgae in photobioreactors for biodiesel application: selection, limitations, and optimization. In: Purohit H, Kalia V, Vaidya A, Khardenavis A (eds) Optimization and applicability of bioprocesses. Springer, Singapore, pp 211–232Google Scholar
  41. 41.
    Gnouma A, Sehli E, Medhioub W, Ben Dhieb R, Masri M, Mehlmer N, Slimani W, Sebai K, Zouari A, Brück T, Medhioub A (2018) Strain selection of microalgae isolated from Tunisian coast: characterization of the lipid profile for potential biodiesel production. Bioprocess Biosyst Eng 41:1449–1459CrossRefGoogle Scholar
  42. 42.
    Huang A, Sun L, Wu S, Liu C, Zhao P, Xie X, Wang G (2017) Utilization of glucose and acetate by Chlorella and the effect of multiple factors on cell composition. J Appl Phycol 29:23–33CrossRefGoogle Scholar
  43. 43.
    Mayers JJ, Nilsson AK, Albers E, Flynn KJ (2017) Nutrients from anaerobic digestion effluents for cultivation of the microalga Nannochloropsis sp. —impact on growth, biochemical composition and the potential for cost and environmental impact savings. Algal Res 26:275–286CrossRefGoogle Scholar
  44. 44.
    Shin DY, Cho HU, Utomo JC, Choi YN, Xu X, Park JM (2015) Biodiesel production from Scenedesmus bijuga grown in anaerobically digested food wastewater effluent. Bioresour Technol 184:215–221CrossRefGoogle Scholar
  45. 45.
    Zhou XP, Xia L, Ge HM, Zhang DL, Hu CX (2013) Feasibility of biodiesel production by microalgae Chlorella sp. (FACHB-1748) under outdoor conditions. Bioresour Technol 138:131–135CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Suvidha Gupta
    • 1
    • 2
  • Sanjay B. Pawar
    • 2
    Email author
  • R. A. Pandey
    • 3
  • Gajanan S. Kanade
    • 4
  • Satish K. Lokhande
    • 4
  1. 1.Academy of Scientific and Innovative Research (AcSIR)GhaziabadIndia
  2. 2.Environmental Biotechnology and Genomics DivisionCSIR-National Environmental Engineering Research Institute (NEERI)NagpurIndia
  3. 3.CSIR-National Environmental Engineering Research Institute (NEERI)NagpurIndia
  4. 4.Analytical Instruments DivisionCSIR-National Environmental Engineering Research Institute (NEERI)NagpurIndia

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