Bioprocess and Biosystems Engineering

, Volume 42, Issue 2, pp 291–296 | Cite as

Removal of aluminium from aqueous solution by four wild-type strains of Aspergillus niger

  • Katarína Boriová
  • Slavomír Čerňanský
  • Peter Matúš
  • Marek Bujdoš
  • Alexandra Šimonovičová
  • Martin UríkEmail author
Research Paper


This paper provides a unique comparison of the performance of four wild-type Aspergillus niger strains in remediation of aluminium(III)-contaminated aqueous solutions. The direct fungal aluminium removal via biosorption and bioaccumulation was compared among all fungal strains, including bioaccumulation efficiency during dynamic and static cultivation. Our results indicate that aluminium bioaccumulation by living biomass outperformed biosorption, although biosorption by non-living biomass is a less time-demanding process. Among others, only one strain significantly differed regarding comparison of dynamic and static bioaccumulation. In this case, a significantly higher removal performance was achieved under dynamic cultivation conditions at initial aluminium(III) concentrations over 2.5 mg L−1. Although the fungal sensitivity towards aluminium(III) differed among selected fungal strains, there was no apparent correlation between the strains’ removal performance and their adaptive mechanisms.


Aluminium Filamentous fungi Biosorption Bioaccumulation Aspergillus niger 



This research was supported by funds obtained from the Scientific Grant Agency of Ministry of Education, Science, Research and Sport of the Slovak Republic and the Slovak Academy of Sciences VEGA Nos. 1/0424/18 and 1/0354/19, and COST IS1408 Industrially Contaminated Sites and Health Network (ICSHNet).

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interest.


  1. 1.
    Dhankhar R, Hooda A (2011) Fungal biosorption—an alternative to meet the challenges of heavy metal pollution in aqueous solutions. Environ Technol 32(5):467–491. CrossRefGoogle Scholar
  2. 2.
    Verma T, Maurya A, Tripathi M, Garg SK (2017) Mycoremediation: an alternative treatment strategy for heavy metal-laden wastewater. In: Satyanarayana T, Deshmukh SK, Johri BN (eds) Developments in fungal biology and applied mycology. Springer, Singapore, pp 315–340. CrossRefGoogle Scholar
  3. 3.
    Mishra V (2014) Biosorption of zinc ion: a deep comprehension. Appl Water Sci 4(4):311–332. CrossRefGoogle Scholar
  4. 4.
    Oladipo OG, Awotoye OO, Olayinka A, Bezuidenhout CC, Maboeta MS (2018) Heavy metal tolerance traits of filamentous fungi isolated from gold and gemstone mining sites. Braz J Microbiol 49(1):29–37. CrossRefGoogle Scholar
  5. 5.
    Gadd GM (1994) Interactions of fungi with toxic metals. In: Powell KA, Renwick A, Peberdy JF (eds) The genus Aspergillus: from taxonomy and genetics to industrial application. Springer, Boston, pp 361–374. CrossRefGoogle Scholar
  6. 6.
    Gadd GM (1993) Interactions of fungi with toxic metals. New Phytol 124(1):25–60. CrossRefGoogle Scholar
  7. 7.
    Sağ Y (2001) Biosorption of heavy metals by fungal biomass and modeling of fungal biosorption: a review. Sep Purif Methods 30(1):1–48. CrossRefGoogle Scholar
  8. 8.
    Benila Smily J, Sumithra RM P, A (2017) Optimization of chromium biosorption by fungal adsorbent, Trichoderma sp. BSCR02 and its desorption studies. Hayati J Biosci 24(2):65–71. CrossRefGoogle Scholar
  9. 9.
    Urík M, Hlodák M, Mikušová P, Matúš P (2014) Potential of microscopic fungi isolated from mercury contaminated soils to accumulate and volatilize mercury(II). Water Air Soil Pollut 225(12):2219. CrossRefGoogle Scholar
  10. 10.
    Šimonovičová A, Nosalj S, Takáčová A, Mackuľak T, Jesenák K, Čerňanský S (2017) Responses of Aspergillus niger to selected environmental factors. Nova Biotechnol Chim 16(2):92–98. CrossRefGoogle Scholar
  11. 11.
    Manrique LA (1986) The relationship of soil pH to aluminum saturation and exchangeable aluminum in ultisols and oxisols. Commun Soil Sci Plant Anal 17(4):439–455. CrossRefGoogle Scholar
  12. 12.
    Šimonovičová A, Hlinková E, Chovanová K, Pangallo D (2013) Influence of the environment on the morphological and biochemical characteristics of different Aspergillus niger wild type strains. Indian J Microbiol 53(2):187–193. CrossRefGoogle Scholar
  13. 13.
    Nováková A (2012) Collection of microscopic fungi ISB—catalogue of strains. Institute of Soil Biology, Biology Centre AS CR, v.v.i., České BudějoviceGoogle Scholar
  14. 14.
    Polák F, Urík M, Bujdoš M, Uhlík P, Matúš P (2018) Evaluation of aluminium mobilization from its soil mineral pools by simultaneous effect of Aspergillus strains’ acidic and chelating exometabolites. J Inorg Biochem 181:162–168. CrossRefGoogle Scholar
  15. 15.
    Gadd GM, Ramsay L, Crawford JW, Ritz K (2006) Nutritional influence on fungal colony growth and biomass distribution in response to toxic metals. FEMS Microbiol Lett 204(2):311–316. CrossRefGoogle Scholar
  16. 16.
    Darlington AB, Rauser WE (1988) Cadmium alters the growth of the ectomycorrhizal fungus Paxillus involutes: a new growth model accounts for changes in branching. Can J Bot 66(2):225–229. CrossRefGoogle Scholar
  17. 17.
    Zwietering MH, Jongenburger I, Rombouts FM, van ‘t Riet K (1990) Modeling of the bacterial growth curve. Appl Environ Microbiol 56(6):1875–1881Google Scholar
  18. 18.
    Zołotajkin M, Ciba J, Kluczka J, Skwira M, Smoliński A (2011) Exchangeable and bioavailable aluminium in the mountain forest soil of Barania Góra range (Silesian Beskids, Poland). Water Air Soil Pollut 216(1–4):571–580. CrossRefGoogle Scholar
  19. 19.
    Jones DL, Prabowo AM, Kochian LV (1996) Aluminium-organic acid interactions in acid soils. Plant Soil 182(2):229–237. CrossRefGoogle Scholar
  20. 20.
    Omeike SO, Kareem SO, Adewuiy S, Balogun SA (2013) Biosorption of aluminium from solution by dead Aspergillus oryzae biomass isolated from aluminium mills waste site. Ife J Sci 15(1):119–124Google Scholar
  21. 21.
    Gáplovská K, Šimonovičová A, Halko R, Okenicová L, Žemberyová M, Čerňanský S, Brandeburová P, Mackuľak T (2018) Study of the binding sites in the biomass of Aspergillus niger wild-type strains by FTIR spectroscopy. Chem Pap 72(9):2283–2288. CrossRefGoogle Scholar
  22. 22.
    Gadd GM (1990) Fungi and yeasts for metal binding. In: Ehrlich H, Brierley CL (eds) Microbial mineral recovery. McGraw-Hill, New York, pp 249–275Google Scholar
  23. 23.
    Boeris PS, Agustín MdR, Acevedo DF, Lucchesi GI (2016) Biosorption of aluminum through the use of non-viable biomass of Pseudomonas putida. J Biotechnol 236:57–63. CrossRefGoogle Scholar
  24. 24.
    Boriová K, Urík M, Bujdoš M, Matúš P (2015) Bismuth(III) volatilization and immobilization by filamentous fungus Aspergillus clavatus during aerobic incubation. Arch Environ Contam Toxicol 68(2):405–411. CrossRefGoogle Scholar
  25. 25.
    Urík M, Bujdoš M, Milová B (2014) Biologically induced mobilization of arsenic adsorbed onto amorphous ferric oxyhydroxides in aqueous solution during fungal cultivation. Water Air Soil Pollut. Google Scholar
  26. 26.
    Boriová K, Urík M, Matúš P (2015) Biosorption, bioaccumulation, biovolatilization of potentially toxic elements by microorganisms. Chem Listy 109(2):109–112Google Scholar
  27. 27.
    Urík M, Boriová K, Bujdoš M, Matúš P (2016) Fungal selenium(VI) accumulation and biotransformation—filamentous fungi in selenate contaminated aqueous media remediation. CLEAN Soil Air Water 44(6):610–614. CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Institute of Laboratory Research on Geomaterials, Faculty of Natural SciencesComenius University in BratislavaBratislavaSlovak Republic
  2. 2.Department of Environmental Ecology, Faculty of Natural SciencesComenius University in BratislavaBratislavaSlovak Republic
  3. 3.Department of Soil Science, Faculty of Natural SciencesComenius University in BratislavaBratislavaSlovak Republic

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