Bioprocess and Biosystems Engineering

, Volume 42, Issue 1, pp 37–46 | Cite as

Microbial calcium carbonate precipitation with high affinity to fill the concrete pore space: nanobiotechnological approach

  • Mostafa Seifan
  • Alireza Ebrahiminezhad
  • Younes Ghasemi
  • Aydin BerenjianEmail author
Research Paper


Despite the advantages of concrete, it has a pore structure and is susceptible to cracking. The initiated cracks as well as pores and their connectivity accelerate the structure degradation by permitting aggressive substances to flow into the concrete matrix. This phenomenon results in a considerable repair and maintenance costs and decreases the concrete lifespan. In recent years, biotechnological approach through immobilization of bacteria in/or protective vehicles has emerged as a viable solution to address this issue. However, the addition of macro- or micro scale size particles can decrease the integrity of matrix. In this study, the immobilization of bacteria with magnetic iron oxide nanoparticle (ION) was proposed to protect the bacterial cell and evaluate their effect on healing the concrete pore space. The results show that the addition of immobilized bacteria with IONs resulted in a lower water absorption and volume of permeable pore space. Crystal analysis using scanning electron microscope (SEM) and energy dispersive X-ray spectroscopy (EDS) revealed that CaCO3 was precipitated in bio-concrete specimen as a result of microbial biosynthesis.


Iron oxide nanoparticle Immobilization CaCO3 Bacteria Concrete Water absorption 



This investigation was financially supported by The University of Waikato, New Zealand.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Ethical approval

This study does not contain any studies with human participants or animals performed by any of the authors.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Mostafa Seifan
    • 1
  • Alireza Ebrahiminezhad
    • 2
  • Younes Ghasemi
    • 2
    • 3
  • Aydin Berenjian
    • 1
    Email author
  1. 1.School of Engineering, Faculty of Science and EngineeringThe University of WaikatoHamiltonNew Zealand
  2. 2.Department of Medical Nanotechnology, School of Advanced Medical Sciences and TechnologiesShiraz University of Medical ScienceShirazIran
  3. 3.Department of Pharmaceutical Biotechnology, School of Pharmacy and Pharmaceutical Sciences Research CentreShiraz University of Medical SciencesShirazIran

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