Bioprocess and Biosystems Engineering

, Volume 42, Issue 1, pp 29–36 | Cite as

Application of high-voltage electrical discharges and high-pressure homogenization for recovery of intracellular compounds from microalgae Parachlorella kessleri

  • Rui Zhang
  • Nabil Grimi
  • Luc Marchal
  • Eugène Vorobiev
Research Paper


Treatments with high-voltage electrical discharges (HVED) and high-pressure homogenization (HPH) were studied and compared for the release of ionic components, carbohydrates, proteins, and pigments from microalgae Parachlorella kessleri (P. kessleri). Suspensions (1% w/w) of microalgae were treated by HVED (40 kV/cm, 1–8 ms) or by HPH (400–1200 bar, 1–10 passes). Particle-size distribution (PSD) and microscopic analyses were used to detect the disruption and damage of cells. HVED were very effective for the extraction of ionic cell components and carbohydrates (421 mg/L after 8 ms of the treatment). However, HVED were ineffective for pigments and protein extraction. The concentration of proteins extracted by HVED was just 750 mg/L and did not exceed 15% of the total quantity of proteins. HPH permitted an effective release overall of intracellular compounds from P. kessleri microalgae including a large quantity of proteins, whose release (at 1200 bar) was 4.9 times higher than that obtained by HVED. Consequently, HVED can be used at the first step of the overall extraction process for the selective recovery of low-molecular-weight components. HPH can be then used at the second step for the recovery of remaining cell compounds.


Microalgae Parachlorella kessleri High-voltage electrical discharges High-pressure homogenization Selective extraction 



Rui Zhang would like to acknowledge the financial support of the China Scholarship Council for thesis fellowship.

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interest.


  1. 1.
    Gong Y, Hu H, Gao Y et al (2011) Microalgae as platforms for production of recombinant proteins and valuable compounds: progress and prospects. J Ind Microbiol Biotechnol 38:1879–1890CrossRefGoogle Scholar
  2. 2.
    Matos CT, Santos M, Nobre BP, Gouveia L (2013) Nannochloropsis sp. biomass recovery by electro-coagulation for biodiesel and pigment production. Bioresour Technol 134:219–226CrossRefGoogle Scholar
  3. 3.
    Parniakov O, Barba FJ, Grimi N et al (2015) Pulsed electric field and pH assisted selective extraction of intracellular components from microalgae nannochloropsis. Algal Res 8:128–134CrossRefGoogle Scholar
  4. 4.
    Barba FJ (2017) Microalgae and seaweeds for food applications: challenges and perspectives. Food Res Int 99:969CrossRefGoogle Scholar
  5. 5.
    Barba FJ, Grimi N, Vorobiev E (2014) New approaches for the use of non-conventional cell disruption technologies to extract potential food additives and nutraceuticals from microalgae. Food Eng Rev 7:45–62CrossRefGoogle Scholar
  6. 6.
    Poojary MM, Barba FJ, Aliakbarian B et al (2016) Innovative alternative technologies to extract carotenoids from microalgae and seaweeds. Mar Drugs 14:214CrossRefGoogle Scholar
  7. 7.
    Günerken E, d’Hondt E, Eppink MHM et al (2015) Cell disruption for microalgae biorefineries. Biotechnol Adv 33:243–260CrossRefGoogle Scholar
  8. 8.
    Luengo E, Martínez JM, Bordetas A et al (2015) Influence of the treatment medium temperature on lutein extraction assisted by pulsed electric fields from Chlorella vulgaris. Innov Food Sci Emerg Technol 29:15–22CrossRefGoogle Scholar
  9. 9.
    Yap BHJ, Dumsday GJ, Scales PJ, Martin GJO (2015) Energy evaluation of algal cell disruption by high pressure homogenisation. Bioresour Technol 184:280–285CrossRefGoogle Scholar
  10. 10.
    Zhang F, Cheng L-H, Xu X-H et al (2011) Screening of biocompatible organic solvents for enhancement of lipid milking from Nannochloropsis sp. Process Biochem 46:1934–1941CrossRefGoogle Scholar
  11. 11.
    Grimi N, Dubois A, Marchal L et al (2014) Selective extraction from microalgae Nannochloropsis sp. using different methods of cell disruption. Bioresour Technol 153:254–259CrossRefGoogle Scholar
  12. 12.
    Norton T, Sun D-W (2008) Recent advances in the use of high pressure as an effective processing technique in the food industry. Food Bioprocess Technol 1:2–34CrossRefGoogle Scholar
  13. 13.
    Patrignani F, Lanciotti R (2016) Applications of high and ultra high pressure homogenization for food safety. Front Microbiol 7:1132Google Scholar
  14. 14.
    Mendes-Pinto MM, Raposo MFJ, Bowen J et al (2001) Evaluation of different cell disruption processes on encysted cells of Haematococcus pluvialis: effects on astaxanthin recovery and implications for bio-availability. J Appl Phycol 13:19–24CrossRefGoogle Scholar
  15. 15.
    Carullo D, Abera BD, Casazza AA et al (2018) Effect of pulsed electric fields and high pressure homogenization on the aqueous extraction of intracellular compounds from the microalgae Chlorella vulgaris. Algal Res 31:60–69CrossRefGoogle Scholar
  16. 16.
    Cooney MJ, Young G, Pate R (2011) Bio-oil from photosynthetic microalgae: case study. Bioresour Technol 102:166–177CrossRefGoogle Scholar
  17. 17.
    Balasundaram B, Harrison S, Bracewell DG (2009) Advances in product release strategies and impact on bioprocess design. Trends Biotechnol 27:477–485CrossRefGoogle Scholar
  18. 18.
    Lee AK, Lewis DM, Ashman PJ (2013) Force and energy requirement for microalgal cell disruption: an atomic force microscope evaluation. Bioresour Technol 128:199–206CrossRefGoogle Scholar
  19. 19.
    Boussetta N, Vorobiev E (2014) Extraction of valuable biocompounds assisted by high voltage electrical discharges: a review. Comptes Rendus Chim 17:197–203CrossRefGoogle Scholar
  20. 20.
    Dubois M, Gilles KA, Hamilton JK et al (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356CrossRefGoogle Scholar
  21. 21.
    Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254CrossRefGoogle Scholar
  22. 22.
    Shynkaryk MV, Lebovka NI, Lanoisellé J-L et al (2009) Electrically-assisted extraction of bio-products using high pressure disruption of yeast cells (Saccharomyces cerevisiae). J Food Eng 92:189–195CrossRefGoogle Scholar
  23. 23.
    Samarasinghe N, Fernando S, Lacey R, Faulkner WB (2012) Algal cell rupture using high pressure homogenization as a prelude to oil extraction. Renew Energy 48:300–308CrossRefGoogle Scholar
  24. 24.
    Spiden EM, Yap BHJ, Hill DRA et al (2013) Quantitative evaluation of the ease of rupture of industrially promising microalgae by high pressure homogenization. Bioresour Technol 140:165–171CrossRefGoogle Scholar
  25. 25.
    Barba FJ, Grimi N, Vorobiev E (2015) Evaluating the potential of cell disruption technologies for green selective extraction of antioxidant compounds from Stevia rebaudiana Bertoni leaves. J Food Eng 149:222–228CrossRefGoogle Scholar
  26. 26.
    Xie Y, Ho S-H, Chen C-NN et al (2016) Disruption of thermo-tolerant Desmodesmus sp. F51 in high pressure homogenization as a prelude to carotenoids extraction. Biochem Eng J 109:243–251CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Rui Zhang
    • 1
  • Nabil Grimi
    • 1
  • Luc Marchal
    • 2
  • Eugène Vorobiev
    • 1
  1. 1.Laboratoire Transformations Intégrées de la Matière Renouvelable (UTC/ESCOM, EA 4297 TIMR) Centre de recherche RoyallieuUniversité de Technologie de Compiègne, Sorbonne UniversitésCompiègne CedexFrance
  2. 2.LUNAM Université, CNRS, GEPEA, Université de Nantes, UMR6144, CRTT, Boulevard de l’UniversitéSaint-Nazaire CedexFrance

Personalised recommendations