Separation and purification of α-ketoglutarate and pyruvate from the fermentation broth of Yarrowia lipolytica
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A strategy to achieve the efficient co-production of α-ketoglutarate (KGA) and pyruvate (PYR) via Yarrowia lipolytica fermentation was established in our previous work. The next big challenge is to achieve an efficient separation of the two keto acids. A strategy for simultaneously separating and purifying KGA and PYR based on their different boiling points was established, leading to the efficient separation and purification of the two keto acids from the fermentation broth of Y. lipolytica. The purity and yield of KGA/PYR reached 99.3/99.5 and 79.8/80.6%, respectively. Application of the separation method on industrial scale could further decrease the cost of the production of the two keto acids by biotechnological routes.
KeywordsKeto acids Yarrowia lipolytica Ethyl acetate Extraction Purification
This work was supported by the National Key Research and Development Program of China (2017YFC1600403), the National Natural Science Foundation of China (Major Program, 21390204), the Key Research and Development Program of Jiangsu Province (BE2016689), the National Natural Science Foundation of China (31670095, 31770097), the Fundamental Research Funds for the Central Universities (JUSRP51701A), the Distinguished Professor Project of Jiangsu Province, the National First-class Discipline Program of Light Industry Technology and Engineering (LITE2018-08), and the 111 Project (111-2-06).
- 14.Yovkova V, Otto C, Aurich A, Mauersberger S, Barth G (2014) Engineering the alpha-ketoglutarate overproduction from raw glycerol by overexpression of the genes encoding NADP+-dependent isocitrate dehydrogenase and pyruvate carboxylase in Yarrowia lipolytica. Appl Microbiol Biotechnol 98:2003–2013CrossRefPubMedGoogle Scholar
- 24.Koo YM, Woo SK, Lee SM, Kim YJ (2006) Recovery of lactic acid from fermentation broth using precipitation and reactive distillation. KSBB J 21:199–203Google Scholar
- 32.Jipa I, Dobre T, Stroescu M, Stoica A (2009) Acetic acid extraction from fermentation broth experimental and modelling studies. Rev Chim 60:1084–1089Google Scholar
- 36.Eda S, Kumari A, Thella PK, Satyavathi B, Rajarathinam P (2017) Recovery of volatile fatty acids by reactive extraction using tri-n-octylamine and tri-butyl phosphate in different solvents: equilibrium studies, pH and temperature effect, and optimization using multivariate taguchi approach. Can J Chem Eng 95:1373–1387CrossRefGoogle Scholar
- 42.Childs SL, Chyall LJ, Dunlap JT, Smolenskaya VN, Stahly BC, Stahly GP (2004) Crystal engineering approach to forming cocrystals of amine hydrochlorides with organic acids. Molecular complexes of fluoxetine hydrochloride with benzoic, succinic, and fumaric acids. J Am Chem Soc 126:13335–13342CrossRefPubMedGoogle Scholar