Bioprocess and Biosystems Engineering

, Volume 41, Issue 10, pp 1497–1508 | Cite as

Enhanced production of succinic acid from methanol–organosolv pretreated Strophanthus preussii by recombinant Escherichia coli

  • Ayobami Matthew Olajuyin
  • Maohua Yang
  • Tingzhen Mu
  • Jiangnan Tian
  • Anders Thygesen
  • Omolola Abidemi Adesanoye
  • Oluwatosin Adekunle Adaramoye
  • Andong SongEmail author
  • Jianmin XingEmail author
Research Paper


A biorefinery process for high yield production of succinic acid from biomass sugars was investigated using recombinant Escherichia coli. The major problem been addressed is utilization of waste biomass for the production of succinic acid using metabolic engineering strategy. Here, methanol extract of Strophanthus preussii was used for fermentation. The process parameters were optimized. Glucose (9 g/L), galactose (4 g/L), xylose (6 g/L) and arabinose (0.5 g/L) were the major sugars present in the methanol extract of S. preussii. E. coli K3OS with overexpression of soluble nucleotide pyridine transhydrogenase sthA and mutation of lactate dehydrogenase A (ldhA), phosphotransacetylase acetate kinase A (pta-ackA), pyruvate formate lyase B (pflB), pyruvate oxidase B (poxB), produced a final succinic acid concentration of 14.40 g/L and yield of 1.10 mol/mol total sugars after 72 h dual-phase fermentation in M9 medium. Here, we show that the maximum theoretical yield using methanol extracts of S. preussii was 64%. Hence, methanol extract of S. preussii could be used for the production of biochemicals such as succinate, malate and pyruvate.


Escherichia coli Succinic acid Fermentation Strophanthus preussii Biorefinery 



This work was supported by National High Technology Research and Development Program of China (863 Project, No 2014AA021905).

Supplementary material

449_2018_1977_MOESM1_ESM.docx (342 kb)
Supplementary material 1 (DOCX 342 KB)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Ayobami Matthew Olajuyin
    • 1
    • 2
  • Maohua Yang
    • 1
  • Tingzhen Mu
    • 1
    • 2
  • Jiangnan Tian
    • 1
    • 2
  • Anders Thygesen
    • 3
    • 4
  • Omolola Abidemi Adesanoye
    • 5
  • Oluwatosin Adekunle Adaramoye
    • 5
  • Andong Song
    • 6
    Email author
  • Jianmin Xing
    • 1
    • 2
    Email author
  1. 1.State Key Laboratory of Biochemical EngineeringInstitute of Process EngineeringBeijingPeople’s Republic of China
  2. 2.University of Chinese Academy of SciencesBeijingPeople’s Republic of China
  3. 3.Department of Chemical and Biochemical EngineeringTechnical University of DenmarkLyngbyDenmark
  4. 4.Sino-Danish Center for Education and ResearchAarhus CDenmark
  5. 5.Department of Biochemistry, Faculty of Basic Medical SciencesUniversity of IbadanIbadanNigeria
  6. 6.Key Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture, College of Life SciencesHenan Agricultural UniversityZhengzhouPeople’s Republic of China

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