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Bioprocess and Biosystems Engineering

, Volume 41, Issue 9, pp 1355–1370 | Cite as

Enhanced production of carotenoids using a Thraustochytrid microalgal strain containing high levels of docosahexaenoic acid-rich oil

  • Hansung Park
  • Minsoo Kwak
  • JeongWoo Seo
  • JeongHyun Ju
  • SunYeon Heo
  • SeungMoon ParkEmail author
  • WonKyung HongEmail author
Research Paper

Abstract

Results to date suggest that microalgal Thraustochytrids family strains can be used to produce high-functional omega-3 rich oil (~ 30–70% of dry cell weight) and carotenoid-based antioxidant pigments simultaneously with value-added bioactive potential. In the present study, we describe the isolation and characterization of a new Thraustochytrid Schizochytrium sp. from the west coastal area of Korea. This newly isolated Thraustochytrid, identified as Schizochytrium sp. through 18S rRNA analysis and named SH104, simultaneously produces high levels of DHA and carotenoid-based antioxidant pigments. An improved Schizochytrium mutant, named SHG104, was obtained from the original host strain by γ-irradiation-induced mutagenesis. Under combined temperature-shift cultivation conditions employing white-light LEDs (light-emitting diodes), Schizochytrium sp. SHG104 yielded 10.8 g L−1 of biomass comprising 45.8% total lipids (32.1% DHA) and 4.6 mg L−1 of astaxanthin. In addition to DHA, the main fatty acids produced by Schizochytrium sp. SHG104 were palmitic acid and a trace of other long-chain fatty acids. The carotenoid profile of SH104 and SHG104 was β-carotene, astaxanthin, canthaxanthin, pheonicoxanthin and echinenone, which analyzed by HPLC and LC/APCI–MS. Furthermore, genomic analysis of Schizochytrium and Aurantiochytrium microalgae confirmed that the presence of carotenogenesis pathway enzymes and genes including geranylgeranyl diphosphate, phytoene synthase, lycopene cyclase, and cytochrome P450 hydroxylase that necessary for the production of antioxidants via a complete biosynthetic KEGG synthesis pathway. This newly isolated Schizochytrium microalga potentially have wide application as a source of antioxidants for astaxanthin-containing pigments, commercial omega-3 lipids and feed additives, such as nutritional supplements for aquaculture.

Keywords

Thraustochytrid Schizochytrium Antioxidant Astaxanthin DHA Carotenogenesis 

Notes

Acknowledgements

This research was supported by a grant from the Next Generation BioGreen 21 project through the Animal Genome Breeding Project Team funded by the Rural Development Administration of Korea (PJ01182502).

Supplementary material

449_2018_1963_MOESM1_ESM.doc (28 kb)
Supplementary material 1 (DOC 27 KB)
449_2018_1963_MOESM2_ESM.tif (133 kb)
Supplementary material 2 (TIF 132 KB)
449_2018_1963_MOESM3_ESM.tif (115 kb)
Supplementary material 3 (TIF 114 KB)
449_2018_1963_MOESM4_ESM.tif (88 kb)
Supplementary material 4 (TIF 87 KB)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Hansung Park
    • 1
  • Minsoo Kwak
    • 2
  • JeongWoo Seo
    • 3
  • JeongHyun Ju
    • 3
  • SunYeon Heo
    • 3
  • SeungMoon Park
    • 1
    Email author
  • WonKyung Hong
    • 1
    Email author
  1. 1.Division of Biotechnology, College of Environmental and Bioresource ScienceChonbuk National UniversityIksanSouth Korea
  2. 2.Department of Chemical and Biomolecular EngineeringKorea Advanced Institute of Science and Technology (KAIST)DaejeonSouth Korea
  3. 3.Applied Microbial Research Center, Jeonbuk Branch InstituteKorea Research Institute of Bioscience and Biotechnology (KRIBB)JeongeupSouth Korea

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