Effects of fundamental nutrient stresses on the lipid accumulation profiles in two diatom species Thalassiosira weissflogii and Chaetoceros muelleri
- 50 Downloads
Microalgae are considered as attractive feedstocks for biofuel production nowadays because of their high lipid contents and easy cultivation. In the present study, two diatoms, Thalassiosira weissflogii and Chaetoceros muelleri, were cultured under various nutrient-limitation conditions to explore their comprehensive lipid accumulation profiles for further commercialization. In T. weissflogii, the highest neutral lipid accumulation and highest lipid productivity (14.28 mg L−1 day−1) were both recorded under P-limitation. In C. muelleri, the highest lipid content (35.03% of dry cell weight), highest neutral lipid accumulation, and highest lipid productivity (29.07 mg L−1 day−1) were all recorded under N-limitation. Besides, the predominant fatty acids of T. weissflogii and C. muelleri were myristic acid (C14:0), palmitic acid (C16:0), and palmitoleic acid (C16:1), with the amounts of 58.4–74.4 and 74.1–87.7% of the total fatty acids, respectively. Moreover, nutrient limitations led to a lower proportion of polyunsaturated fatty acids (PUFA) than that of saturated fatty acid (SFA) and monounsaturated fatty acid (MUFA) in both species. The ratios of (SFA + MUFA) to PUFA were from 1.65 to 3.01 in T. weissflogii, and up to 3.61 to 8.59 in C. muelleri. Our results suggested the feasibility of C. muelleri as biodiesel feedstock due to its more suitable fatty acid composition and higher lipid productivity compared to T. weissflogii.
KeywordsDiatom Lipid accumulation profiles and biofuel Nutrient limitation Thalassiosira weissflogii Chaetoceros muelleri
This work was supported by the National 973 project under Grant number 2011CB200901, and the National Natural Science Foundation of China (Grant nos. 41576138 and 41276130).
Compliance with ethical standards
Conflict of interest
We declare that we have no conflict of interest to this work.
- 9.Merzlyak M, Chivkunova O, Gorelova O, Reshetnikova I, Solovchenko A, Khozin-Goldberg I, Cohen Z (2007) Effect of nitrogen starvation on optical properties, pigments, and arachidonic acid content of the unicellular green alga Parietochloris incisa (Trebouxiophyceae, Chlorophyta). J Phycol 43(4):833–843CrossRefGoogle Scholar
- 14.Miller R, Wu G, Deshpande R, Vieler A, Gärtner K, Li X, Moellering E, Zäuner S, Cornish A, Liu B, Bullard B, Sears B, Kuo M, Hegg E, Shachar-Hill Y, Shiu S, Benning C (2010) Changes in transcript abundance in Chlamydomonas reinhardtii following nitrogen deprivation predict diversion of metabolism. Plant Physiol 154(4):1737–1752CrossRefGoogle Scholar
- 22.Wang X, Liang J, Luo C, Chen C, Gao Y (2014) Biomass, total lipid production, and fatty acid composition of the marine diatom Chaetoceros muelleri in response to different CO2 levels. Bioresour Technol 161(6):124–130Google Scholar
- 25.Hallegraeff G, Anderson D, Cembella A, Enevoldsen H, Commission I (2003) Manual on harmful marine microalgae. UNESCO, ParisGoogle Scholar
- 26.Guillard R (2005) Purification methods for microalgae. In: Andersen RA (ed) Phycological methods: algal culturing techniques. Elsevier Academic Press, Burlington, pp 117–132Google Scholar