Bioprocess and Biosystems Engineering

, Volume 41, Issue 8, pp 1185–1193 | Cite as

Soluble lipase-catalyzed synthesis of methyl esters using a blend of edible and nonedible raw materials

  • João H. C. Wancura
  • Daniela V. Rosset
  • Michel Brondani
  • Marcio A. Mazutti
  • J. Vladimir Oliveira
  • Marcus V. TresEmail author
  • Sérgio L. Jahn
Research Paper


This work investigates the use of blends of edible and nonedible raw materials as an alternative feedstock to fatty acid methyl esters (FAME) production through enzymatic catalysis. As biocatalyst, liquid lipase from Thermomyces lanuginosus (Callera™ Trans L), was used. Under reaction conditions of 35 °C, methanol to feedstock molar ratio of 4.5:1 and 1.45% of catalyst load, the best process performance was reached using 9% of water concentration in the medium—yield of 79.9% after 480 min of reaction. In terms of use of tallow mixed with soybean oil, the best yield was obtained when 100% of tallow was used in the process—84.6% after 480 min of reaction—behavior that was associated with the degree of unsaturation of the feedstock, something by that time, not addressed in papers of the area. The results show that tallow can be used as an alternative to FAME production, catalyzed by soluble lipase.


FAME Tallow Callera™ Trans L Degree of unsaturation Transesterification 



The authors would like to thank the National Council of Technological and Science Development (CNPq), Research Support Foundation of the State of Rio Grande do Sul (FAPERGS) and Coordination for the Improvement of Higher Education Personnel (CAPES) for the financial support for this project.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Chemical EngineeringFederal University of Santa MariaSanta MariaBrazil
  2. 2.Department of Chemical and Food EngineeringFederal University of Santa CatarinaFlorianópolisBrazil
  3. 3.Laboratory of Agroindustrial Processes Engineering (LAPE)Federal University of Santa MariaCachoeira do SulBrazil

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