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Bioprocess and Biosystems Engineering

, Volume 42, Issue 4, pp 603–610 | Cite as

Polyhydroxybutyrate production in halophilic marine bacteria Vibrio proteolyticus isolated from the Korean peninsula

  • Ju-Won Hong
  • Hun-Suk Song
  • Yu-Mi Moon
  • Yoon-Gi Hong
  • Shashi Kant Bhatia
  • Hye-Rim Jung
  • Tae-Rim Choi
  • Soo-yeon Yang
  • Hyung-Yeon Park
  • Yong-Keun Choi
  • Yung-Hun YangEmail author
Research Paper

Abstract

Polyhydroxybutyrates (PHB) are biodegradable polymers that are produced by various microbes, including Ralstonia, Pseudomonas, and Bacillus species. In this study, a Vibrio proteolyticus strain, which produces a high level of polyhydroxyalkanoate (PHA), was isolated from the Korean marine environment. To determine optimal growth and production conditions, environments with different salinity, carbon sources, and nitrogen sources were evaluated. We found that the use of a medium containing 2% (w/v) fructose, 0.3% (w/v) yeast extract, and 5% (w/v) sodium chloride (NaCl) in M9 minimal medium resulted in high PHA content (54.7%) and biomass (4.94 g/L) over 48 h. Addition of propionate resulted in the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (P(HB-co-HV)) copolymer as propionate acts as a precursor for the HV unit. In these conditions, the bacteria produced poly(3-hydroxybutyrate-co-3-hydroxyvalerate) containing a 15.8% 3HV fraction with 0.3% propionate added as the substrate. To examine the possibility of using unsterilized media with high NaCl content for PHB production, V. proteolyticus was cultured in sterilized and unsterilized conditions. Our results indicated a higher growth, leading to a dominant population in unsterilized conditions and higher PHB production. This study showed the conditions for halophilic PHA producers to be later implemented at a larger scale.

Keywords

Polyhydroxyalkanoate Bioplastic Marine bacteria Halophilic bacteria 

Notes

Acknowledgements

This study was supported by the National Research Foundation of Korea (NRF) (NRF-2015M1A5A1037196, 2016R1D1A1B03932163, NRF-2017R1D1A1B03033594) and Polar Academic Program (PAP, PE18900). This paper was also supported by Konkuk University Researcher Fund in 2017. Consulting service from the Microbial Carbohydrate Resource Bank (MCRB, Seoul, Korea) was kindly appreciated.

Supplementary material

449_2018_2066_MOESM1_ESM.doc (524 kb)
Supplementary material 1 (DOC 524 KB)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Ju-Won Hong
    • 1
  • Hun-Suk Song
    • 1
  • Yu-Mi Moon
    • 1
  • Yoon-Gi Hong
    • 1
  • Shashi Kant Bhatia
    • 1
    • 2
  • Hye-Rim Jung
    • 1
  • Tae-Rim Choi
    • 1
  • Soo-yeon Yang
    • 1
  • Hyung-Yeon Park
    • 1
  • Yong-Keun Choi
    • 1
  • Yung-Hun Yang
    • 1
    Email author
  1. 1.Department of Biological Engineering, College of EngineeringKonkuk UniversitySeoulRepublic of Korea
  2. 2.Institute for Ubiquitous Information Technology and Applications (CBRU)Konkuk UniversitySeoulRepublic of Korea

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