Micelle-mediated transport disturbance providing extracellular strategy for alleviating n-butanol stress on Escherichia coli
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One barrier inhibiting further progress in biofuel production is the toxicity of biofuels towards their producers. It is promising to apply gene-based intracellular techniques to engineer better strains with higher organic solvent tolerance. These methods are, however, complex. In the present study, we developed a simple, manageable, and commercial extracellular prototypal strategy to alleviate n-butanol (n-BuOH) stress on Escherichia coli via a micelle-mediated transport disturbance. When the concentration of sodium dodecyl sulfate, a typical anionic surfactant, is high enough to form micelles, n-BuOH will be trapped into/onto the micelles, and the negative charge prevents the n-BuOH from approaching the cells. Our study provides an extracellular strategy to relieve the stress from n-BuOH, and it also exhibits a new angle to advance microbial factories through extracellular routines.
KeywordsBiofuels Micelles n-Butanol Organic solvent tolerance Surfactants
This work was supported by the National Natural Science Foundation of China (no. 21476130).
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Conflict of interest
The authors declare that they have no conflict of interest.