Bioprocess and Biosystems Engineering

, Volume 40, Issue 12, pp 1763–1773 | Cite as

Scale-up cultivation enhanced arachidonic acid accumulation by red microalgae Porphyridium purpureum

  • Jingyu Chang
  • Kai Le
  • Xiaoqiang Song
  • Kailin Jiao
  • Xianhai Zeng
  • Xueping Ling
  • Tuo Shi
  • Xing Tang
  • Yong Sun
  • Lu Lin
Research Paper

Abstract

The present study attempts to cultivate Porphyridium purpureum under different scale-up conditions for further development and commercialization of microalgae-derived PUFAs such as ARA and EPA. Different temperatures (25, 30, and 35 °C) and light intensities (70, 165, and 280 μmol/m2s) were applied to the 50 L pilot-scale cultivation of P. purpureum in ASW. The cultivation under the light intensity of 280 μmol/m2s at 35 °C obtained biomass concentration up to 9.52 g/L, total fatty acid content to 56.82 mg/g, and ARA content to 22.29 mg/g. While the maximum EPA content of 7.00 mg/g was achieved under the light intensity of 280 μmol/m2s at 25 °C and the highest ratio of UFAs to TFAs of 74.66% was also obtained in this trial. Both biomass concentration and TFAs content were improved by increasing light intensity and temperature. Moreover, the ratio of ARA to EPA was enhanced by increasing cultivation temperature under the light intensity of 280 μmol/m2s. In contrast with flask culture, the conversion of linoleic acid (C18:2) to ARA was enhanced in scale-up culture, leading to more ARA content. Phosphate limitation enhanced the synthesis of lipid and LPUFAs. Moreover, the biomass concentration and biosynthesis of palmitic acid were preferred by sufficient C (NaHCO3).

Graphical abstract

Keywords

Scale-up cultivation Enhance Arachidonic acid Phosphate limitation Porphyridium purpureum 

Supplementary material

449_2017_1831_MOESM1_ESM.doc (227 kb)
Supplementary material 1 (DOC 227 kb)

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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Jingyu Chang
    • 1
  • Kai Le
    • 1
  • Xiaoqiang Song
    • 1
  • Kailin Jiao
    • 1
  • Xianhai Zeng
    • 1
    • 4
  • Xueping Ling
    • 2
  • Tuo Shi
    • 3
  • Xing Tang
    • 1
    • 4
  • Yong Sun
    • 1
    • 4
  • Lu Lin
    • 1
    • 4
  1. 1.College of EnergyXiamen UniversityXiamenPeople’s Republic of China
  2. 2.Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical EngineeringXiamen UniversityXiamenPeople’s Republic of China
  3. 3.State Key Laboratory of Marine Environmental Science, College of Ocean and Earth SciencesXiamen UniversityXiamenPeople’s Republic of China
  4. 4.Xiamen Key Laboratory of High-valued Conversion Technology of Agricultural BiomassXiamen UniversityXiamenPeople’s Republic of China

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