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Bioprocess and Biosystems Engineering

, Volume 40, Issue 6, pp 857–866 | Cite as

Change of Monascus pigment metabolism and secretion in different extractive fermentation process

  • Gong Chen
  • Rui Tang
  • Xiaofei Tian
  • Peng Qin
  • Zhenqiang WuEmail author
Research Paper

Abstract

Monascus pigments that were generally produced intracellularly from Monascus spp. are important natural colorants in food industry. In this study, change of pigment metabolism and secretion was investigated through fed-batch extractive fermentation and continuous extractive fermentation. The biomass, secreting rate of pigment and total pigment yield closely correlated with the activated time of extractive fermentation as well as the composition of feeding nutrients. Metal ions played a key role in both the cell growth and pigment metabolism. Nitrogen source was necessary for a high productivity of biomass but not for high pigment yield. Furthermore, fermentation period for the fed-batch extractive fermentation could be reduced by 18.75% with a nitrogen source free feeding medium. Through a 30-day continuous extractive fermentation, the average daily productivity for total pigments reached 74.9 AU day−1 with an increase by 32.6 and 296.3% compared to that in a 6-day conventional batch fermentation and a 16-day fed-batch extractive fermentation, respectively. At the meantime, proportions of extracellular pigments increased gradually from 2.7 to 71.3%, and yellow pigments gradually became dominated in both intracellular and extracellular pigments in the end of continuous extractive fermentation. This findings showed that either fed-batch or continuous extractive fermentation acted as a promising method in the efficient production of Monascus pigments.

Keywords

Monascus pigment Extractive fermentation Extracellular secretion Spectral characteristics Nonionic surfactant 

Notes

Acknowledgements

The authors gratefully acknowledge the financial support of the National Natural Science Foundation of China (No. 31271925), the Special Project on the Integration of Industry, Education and Research of Guangdong Province, China (No. 2013B090600015), and the Science and Technology Program of Guangzhou, China (No. 2014J4100192).

Compliance with ethical standards

Conflict of interest

The authors claim no conflict of interest.

References

  1. 1.
    Srianta I, Ristiarini S, Nugerahani I, Sen SK, Zhang BB, Xu GR, Blanc PJ (2014) Recent research and development of Monascus fermentation products. Int Food Res J 1:1–12Google Scholar
  2. 2.
    Lin YL, Wang TH, Lee MH, Su NW (2008) Biologically active components and nutraceuticals in the Monascus-fermented rice: a review. Appl Microbiol Biotechnol 77:965–973CrossRefGoogle Scholar
  3. 3.
    Feng Y, Shao Y, Chen F (2012) Monascus pigments. Appl Microbiol Biotechnol 96:1421–1440CrossRefGoogle Scholar
  4. 4.
    Subhasree RS, Babu PD, Vidyalakshmi R, Mohan VC (2011) Effect of carbon and nitrogen sources on stimulation of pigment production by Monascus purpureus on jackfruit seeds. Int J Microbiol Res 2:184–187Google Scholar
  5. 5.
    Hamdi M, Blanc PJ, Loret MO, Goma G (1997) A new process for red pigment production by submerged culture of Monascus purpureus. Bioproc Biosyst Eng 17:75–79Google Scholar
  6. 6.
    Hsu FL, Wang PM, Lu SY, Wu WT (2002) A combined solid-state and submerged cultivation integrated with adsorptive product extraction for production of Monascus red pigments. Bioproc Biosyst Eng 25:165–168CrossRefGoogle Scholar
  7. 7.
    Pereira DG, Tonso A, Kilikian BV (2008) Effect of dissolved oxygen concentration on red pigment and citrinin production by Monascus purpureus ATCC 36928. Braz J Chem Eng 25:247–253CrossRefGoogle Scholar
  8. 8.
    Tudor D, Robinson SC, Cooper PA (2013) The influence of pH on pigment formation by lignicolous fungi. Int Biodeterior Biodegrad 80:22–28CrossRefGoogle Scholar
  9. 9.
    Lee J, Lee SY, Park S, Middelberg APJ (1999) Control of fed-batch fermentations. Biotechnol Adv 17:29–48CrossRefGoogle Scholar
  10. 10.
    Hu Z, Zhang X, Wu Z, Qi H, Wang Z (2012) Perstraction of intracellular pigments by submerged cultivation of Monascus in nonionic surfactant micelle aqueous solution. Appl Microbiol Biotechnol 94:81–89CrossRefGoogle Scholar
  11. 11.
    Hu M, Zhang X, Wang Z (2016) Releasing intracellular product to prepare whole cell biocatalyst for biosynthesis of Monascus pigments in water-edible oil two-phase systems. Bioproc Biosyst Eng 39:1785–1791CrossRefGoogle Scholar
  12. 12.
    Wang Z, Dai Z (2010) Extractive microbial fermentation in cloud point system. Enzyme Microb Technol 46:407–418CrossRefGoogle Scholar
  13. 13.
    Kang B, Zhang X, Wu Z, Qi H, Wang Z (2013) Solubilization capacity of nonionic surfactant micelles exhibiting strong influence on export of intracellular pigments in Monascus fermentation. Microb Biotechnol 6:540–550CrossRefGoogle Scholar
  14. 14.
    Wang Y, Zhang B, Lu L, Huang Y, Xu G (2013) Enhanced production of pigments by addition of surfactants in submerged fermentation of Monascus purpureus H1102. J Sci Food Agric 93:3339–3344CrossRefGoogle Scholar
  15. 15.
    Kang B, Zhang X, Wu Z, Qi H, Wang Z (2013) Effect of pH and nonionic surfactant on profile of intracellular and extracellular Monascus pigments. Process Biochem 48:759CrossRefGoogle Scholar
  16. 16.
    Shen L, Zhang X, Liu M, Wang Z (2014) Transferring of red Monascus pigments from nonionic surfactant to hydrophobic ionic liquid by novel microemulsion extraction. Sep Purif Technol 138:34–40CrossRefGoogle Scholar
  17. 17.
    Hu Z, Zhang X, Wu Z, Qi H, Wang Z (2012) Export of intracellular Monascus pigments by two-stage microbial fermentation in nonionic surfactant micelle aqueous solution. J Biotechnol 162:202–209CrossRefGoogle Scholar
  18. 18.
    Wang B, Zhang X, Wu Z, Wang Z (2016) Biosynthesis of Monascus pigments by resting cell submerged culture in nonionic surfactant micelle aqueous solution. Appl Microbiol Biot 100:7083–7089CrossRefGoogle Scholar
  19. 19.
    Ghaly AE, Kamal M, Correia LR (2005) Kinetic modeling of continuous submerged fermentation of cheese whey for single cell protein production. Bioresour Technol 96:1143–1152CrossRefGoogle Scholar
  20. 20.
    Brethauer S, Wyman CE (2010) Review: continuous hydrolysis and fermentation for cellulosic ethanol production. Bioresour Technol 101:4862–4874CrossRefGoogle Scholar
  21. 21.
    Zheng Y, Xin Y, Guo Y (2009) Study on the fingerprint profile of Monascus products with HPLC–FD, PAD and MS. Food Chem 113:705–711CrossRefGoogle Scholar
  22. 22.
    Chen G, Shi K, Song D, Quan L, Wu Z (2015) The pigment characteristics and productivity shifting in high cell density culture of Monascus anka mycelia. BMC Biotechnol 15:72CrossRefGoogle Scholar
  23. 23.
    Zhao F, Yu J (2001) L-Asparaginase release from Escherichia coli cells with K2HPO4 and Triton X100. Biotechnol Prog 17:490–494CrossRefGoogle Scholar
  24. 24.
    Shi K, Song D, Chen G, Pistolozzi M, Wu Z, Quan L (2015) Controlling composition and color characteristics of Monascus pigments by pH and nitrogen sources in submerged fermentation. J Biosci Bioeng 120:145–154CrossRefGoogle Scholar
  25. 25.
    Riesenberg D, Guthke R (1999) High-cell-density cultivation of microorganisms. Appl Microbiol Biotechnol 51:422–430CrossRefGoogle Scholar
  26. 26.
    Yang J, Chen Q, Wang W, Hu J, Hu C (2015) Effect of oxygen supply on Monascus pigments and citrinin production in submerged fermentation. J Biosci Bioeng 119:564–569CrossRefGoogle Scholar
  27. 27.
    Musaalbakri AM, Ariff A, Rosfarizan M, Ismail A (2006) Aeration and agitation strategies for the improvement of red pigment production by Monascus purpureus FTC 5391. J Trop Agric Food Sci 34:89Google Scholar
  28. 28.
    Foster JW, Wanksman SA (1939) The specific effect of zinc and other heavy metals on growth and fumaric-acid production by Rhizopus. J Bacteriol 37:599–617Google Scholar
  29. 29.
    Cane DE (1984) In: Suckling CJ (ed) Enzyme Chemistry, Enzyme-level studies of the biosynthesis of natural products. Springer, Netherlands, pp 196–231Google Scholar
  30. 30.
    Lin TF, Demain AL (1991) Effect of nutrition of Monascus sp. on formation of red pigments. Appl Microbiol Biotechnol 36:70–75CrossRefGoogle Scholar
  31. 31.
    Hajjaj H, Klaebe A, Goma G, Blanc PJ, Barbier E, Francois J (2000) Medium-chain fatty acids affect citrinin production in the filamentous fungus Monascus ruber. Appl Environ Microbiol 66:1120–1125CrossRefGoogle Scholar
  32. 32.
    Carels M, Shepherd D (1977) The effect of different nitrogen sources on pigment production and sporulation of Monascus species in submerged, shaken culture. Can J Microbiol 23:1360–1372CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  • Gong Chen
    • 1
  • Rui Tang
    • 1
  • Xiaofei Tian
    • 1
    • 3
  • Peng Qin
    • 2
  • Zhenqiang Wu
    • 1
    Email author
  1. 1.School of Bioscience and BioengineeringSouth China University of TechnologyGuangzhouChina
  2. 2.Guangzhou Institute of MicrobiologyGuangzhouChina
  3. 3.Dongguan Tianyi Biotechnology Co., LtdDongguanChina

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