Bioprocess and Biosystems Engineering

, Volume 40, Issue 3, pp 395–402 | Cite as

Use of saline waste water from demineralization of cheese whey for cultivation of Schizochytrium limacinum PA-968 and Japonochytrium marinum AN-4

  • Tomas Humhal
  • Petr Kastanek
  • Zuzana Jezkova
  • Anna Cadkova
  • Jana Kohoutkova
  • Tomas Branyik
Research Paper

Abstract

Saline waste water from demineralization of cheese whey was used as the main component of waste saline medium (WSM) for cultivation of thraustochytrids. The suitability of WSM for cultivation of Schizochytrium limacinum PA-968 and Japonochytrium marinum AN-4 was evaluated by comparison with cultivation on nutrient medium (NM) in shake flask and fermenter cultures. Biomass productivities achieved in WSM for the thraustochytrids were comparable with those in NM for both shake flask and fermenter cultures. The maximum total lipid content (56.71% dry cell weight) and docosahexaenoic acid productivity (0.86 g/L/day) were achieved by J. marinum AN-4 grown on WSM in shake flask and fermenter cultures, respectively. A cost estimate of WSM suggests that this medium could result in lower production costs for thraustochytrid biomass and lipids and contribute to the effective reduction in saline diary process waste water.

Keywords

Saline waste water Schizochytrium limacinum Japonochytrium marinum Docosahexaenoic acid Shake flask Bioreactor 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Tomas Humhal
    • 1
  • Petr Kastanek
    • 1
    • 2
  • Zuzana Jezkova
    • 1
  • Anna Cadkova
    • 1
  • Jana Kohoutkova
    • 3
  • Tomas Branyik
    • 1
  1. 1.Department of BiotechnologyUniversity of Chemistry and Technology PraguePragueCzech Republic
  2. 2.EcoFuel Laboratories Ltd.PragueCzech Republic
  3. 3.Department of Food Analysis and NutritionUniversity of Chemistry and Technology PraguePragueCzech Republic

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