Bioprocess and Biosystems Engineering

, Volume 40, Issue 2, pp 161–180 | Cite as

High-throughput strategies for the discovery and engineering of enzymes for biocatalysis

  • Philippe Jacques
  • Max Béchet
  • Muriel Bigan
  • Delphine Caly
  • Gabrielle Chataigné
  • François Coutte
  • Christophe Flahaut
  • Egon Heuson
  • Valérie Leclère
  • Didier Lecouturier
  • Vincent Phalip
  • Rozenn Ravallec
  • Pascal Dhulster
  • Rénato Froidevaux
Mini Review

Abstract

Innovations in novel enzyme discoveries impact upon a wide range of industries for which biocatalysis and biotransformations represent a great challenge, i.e., food industry, polymers and chemical industry. Key tools and technologies, such as bioinformatics tools to guide mutant library design, molecular biology tools to create mutants library, microfluidics/microplates, parallel miniscale bioreactors and mass spectrometry technologies to create high-throughput screening methods and experimental design tools for screening and optimization, allow to evolve the discovery, development and implementation of enzymes and whole cells in (bio)processes. These technological innovations are also accompanied by the development and implementation of clean and sustainable integrated processes to meet the growing needs of chemical, pharmaceutical, environmental and biorefinery industries. This review gives an overview of the benefits of high-throughput screening approach from the discovery and engineering of biocatalysts to cell culture for optimizing their production in integrated processes and their extraction/purification.

Keywords

Biotechnologies Characterization Enzyme catalysis High-throughput screening 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Philippe Jacques
    • 1
  • Max Béchet
    • 1
  • Muriel Bigan
    • 1
  • Delphine Caly
    • 1
  • Gabrielle Chataigné
    • 1
  • François Coutte
    • 1
  • Christophe Flahaut
    • 1
  • Egon Heuson
    • 1
  • Valérie Leclère
    • 1
  • Didier Lecouturier
    • 1
  • Vincent Phalip
    • 1
  • Rozenn Ravallec
    • 1
  • Pascal Dhulster
    • 1
  • Rénato Froidevaux
    • 1
  1. 1.Equipe Procédés Biologiques, Génie Enzymatique et Microbien, ProBioGEM, Institut Charles Viollette, E.A. 7394Université de Lille et Université d’ArtoisVilleneuve d’AscqFrance

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