Bioprocess and Biosystems Engineering

, Volume 40, Issue 1, pp 1–7 | Cite as

Immobilization of proline-specific endoprotease on nonporous silica nanoparticles functionalized with amino group

  • Fuhua Zhao
  • Tonggang HouEmail author
  • Jianxun Wang
  • Yijun Jiang
  • Shuxia Huang
  • Qiao Wang
  • Mo Xian
  • Xindong MuEmail author
Original Paper


Enzyme immobilization is believed to provide an excellent base for increasing environmental tolerance of enzyme and considerable period of time. In this work, a kind of nonporous silica nanoparticles functionalized with amino group was synthesized to immobilize proline-specific endoprotease (PSEP). PSEP is known to specifically cleave peptides (or esters) at the carboxyl side of proline, thus can prevent the formation of haze and prolong the shelf life of beer. After immobilization, the environmental tolerance (temperature and pH, respectively) was obviously improved, and the immobilized enzyme can retain above 90 % of its original activity after 6 uses. Moreover, the immobilized enzyme can effectively prevent the formation of chill-haze using fresh beer fermentation liquid.


Enzyme immobilization Proline-specific endoprotease Enzymatic activity Beer Chill-haze 



This work is supported by the National Natural Science Foundation of China (Nos. 21433001, 21273260), Applied Basic Research Foundation of Qingdao City (No. 14-2-4-21-jch), and Shandong Provincial Natural Science Foundation for Distinguished Young Scholar, China (No. JQ201305).


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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Fuhua Zhao
    • 1
    • 2
  • Tonggang Hou
    • 2
    Email author
  • Jianxun Wang
    • 2
  • Yijun Jiang
    • 2
  • Shuxia Huang
    • 1
  • Qiao Wang
    • 2
  • Mo Xian
    • 2
  • Xindong Mu
    • 2
    Email author
  1. 1.State Key Laboratory of Biological Fermentation Engineering of BeerTsingtao Brewery Co., LtdQingdaoPeople’s Republic of China
  2. 2.Key Laboratory of Bio-based Materials, Qingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of SciencesQingdaoPeople’s Republic of China

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