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Highly efficient production of d-lactic acid from chicory-derived inulin by Lactobacillus bulgaricus

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Abstract

Inulin is a readily available feedstock for cost-effective production of biochemicals. To date, several studies have explored the production of bioethanol, high-fructose syrup and fructooligosaccharide, but there are no studies regarding the production of d-lactic acid using inulin as a carbon source. In the present study, chicory-derived inulin was used for d-lactic acid biosynthesis by Lactobacillus bulgaricus CGMCC 1.6970. Compared with separate hydrolysis and fermentation processes, simultaneous saccharification and fermentation (SSF) has demonstrated the best performance of d-lactic acid production. Because it prevents fructose inhibition and promotes the complete hydrolysis of inulin, the highest d-lactic acid concentration (123.6 ± 0.9 g/L) with a yield of 97.9 % was obtained from 120 g/L inulin by SSF. Moreover, SSF by L. bulgaricus CGMCC 1.6970 offered another distinct advantage with respect to the higher optical purity of d-lactic acid (>99.9 %) and reduced number of residual sugars. The excellent performance of d-lactic acid production from inulin by SSF represents a high-yield method for d-lactic acid production from non-food grains.

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Acknowledgments

This study was supported by the Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, the National Natural Science Foundation of China (51561145015), the Key Research and Development Program of Jiangsu Province of China (BF2015007).The authors are also grateful to the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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Authors and Affiliations

  1. College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, People’s Republic of China

    Qianqian Xu, Jie Zhou, Xin Li & Jia Ouyang

  2. College of Forestry, Nanjing Forestry University, Nanjing, 210037, People’s Republic of China

    Ying Zang & Peng Liu

  3. Key Laboratory of Forest Genetics and Biotechnology of the Ministry of Education, Nanjing Forestry University, Nanjing, 210037, People’s Republic of China

    Qiang Yong & Jia Ouyang

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  1. Qianqian Xu
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  2. Ying Zang
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  3. Jie Zhou
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  4. Peng Liu
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  5. Xin Li
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Correspondence to Jia Ouyang.

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Xu, Q., Zang, Y., Zhou, J. et al. Highly efficient production of d-lactic acid from chicory-derived inulin by Lactobacillus bulgaricus . Bioprocess Biosyst Eng 39, 1749–1757 (2016). https://doi.org/10.1007/s00449-016-1650-5

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  • DOI: https://doi.org/10.1007/s00449-016-1650-5

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