Abstract
The evolutionary adaptation was carried out on the thermotolerant yeast Kluyveromyces marxianus NIRE-K1 at 45 °C up to 60 batches to enhance its xylose utilization capability. The adapted strain showed higher specific growth rate and 3-fold xylose uptake rate and short lag phase as compared to the native strain. During aerobic growth adapted yeast showed 2.81-fold higher xylose utilization than that of native. In anaerobic batch fermentation, adapted yeast utilized about 91 % of xylose in 72 h and produced 2.88 and 18.75 g l−1 of ethanol and xylitol, respectively, which were 5.11 and 5.71-fold higher than that of native. Ethanol yield, xylitol yield and specific sugar consumption rate obtained by the adapted cells were found to be 1.57, 1.65 and 4.84-fold higher than that of native yeast, respectively. Aforesaid results suggested that the evolutionary adaptation will be a very effective strategy in the near future for economic lignocellulosic ethanol production.
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One of the authors (N. K. Sharma) is very thankful to Sardar Swaran Singh National Institute of Bio-Energy, Kapurthala for providing Junior Research Fellowship and I. K. Gujral Punjab Technical University, Kapurthala for providing Ph.D. registration (Pro. reg. 1422002). Authors are also gratefully acknowledged the Ministry of New and Renewable Energy, Govt. of India for providing financial supports to carry out the research activities.
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Sharma, N.K., Behera, S., Arora, R. et al. Enhancement in xylose utilization using Kluyveromyces marxianus NIRE-K1 through evolutionary adaptation approach. Bioprocess Biosyst Eng 39, 835–843 (2016). https://doi.org/10.1007/s00449-016-1563-3
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DOI: https://doi.org/10.1007/s00449-016-1563-3