Skip to main content
SpringerLink
Log in

Growth of Ag-nanoparticles in an aqueous solution and their antimicrobial activities against Gram positive, Gram negative bacterial strains and Candida fungus

  • Original Paper
  • Published:
Bioprocess and Biosystems Engineering Aims and scope Submit manuscript

Abstract

Silver nanoparticles (AgNPs) were synthesized using Ocimum sanctum (Tulsi) leaves aqueous extract as reducing as well as a capping agent in absence and presence of cetyltrimethylammonium bromide (CTAB). The resulting nanomaterials were characterized by UV–visible spectrophotometer, and transmission electron microscope. The UV–Vis spectroscopy revealed the formation of AgNPs at 400–450 nm. TEM photographs indicate that the truncated triangular silver nanoplates and/or spherical morphology of the AgNPs with an average diameter of 25 nm have been distorted markedly in presence of CTAB. The AgNPs were almost mono disperse in nature. Antimicrobial activities of AgNPs were determined by using two bacteria (Gram positive Staphylococcus aureus MTCC-3160), Gram negative Escherichia coli MTCC-450) and one species of Candida fungus (Candida albicans ATCC 90030) with Kirby-Bauer or disc diffusion method. The zone of inhibition seems extremely good showing a relatively large zone of inhibition in both Staphylococcus aureus, Escherichia coli, and Candida albicans strains.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Scheme 1
Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Scheme 2
Fig. 6

Similar content being viewed by others

References

  1. Henglein A (1989) Chem Rev 89:1861–1873

    Article  CAS  Google Scholar 

  2. Henglein A (1993) J Phys Chem 97:5457–5471

    Article  CAS  Google Scholar 

  3. Pal T, Sau TK, Jana NR (1997) Langmuir 13:1481–1485

    Article  CAS  Google Scholar 

  4. El-Sayed MA (2001) Acc Chem Res 34:257264

    Article  Google Scholar 

  5. Rosi NL, Mirkin CA (2005) Chem Rev 105:1547–1562

    Article  CAS  Google Scholar 

  6. Xie J, Lee JY, Wang DIC, Ting YP (2007) ACS Nano 1:429–439

    Article  CAS  Google Scholar 

  7. Bakshi MS (2010) J Nanosci Nanotechnol 10:1757–1765

    Article  CAS  Google Scholar 

  8. Khan Z, Singh T, Hussain JI, Al-Thabaiti SA, El-Mossalamy EH, Obaid AY (2013) Colloids Surf B: Biointerfaces 102:578–584

    Article  CAS  Google Scholar 

  9. Solomon SD, Bahadory M, Jeyarajasingam AV, Rutkowsky SA, Boritz C (2007) J Chem Edu 84:322–325

    Article  CAS  Google Scholar 

  10. Kim HS, Ryu JH, Jose B, Lee BG, Ahn BS, Kang YS (2001) Langmuir 17:5817–5820

    Article  CAS  Google Scholar 

  11. Zhu Y, Li H, Koltypin Y, Gedanken AJ (2002) Mater Chem 12:729–733

    Article  CAS  Google Scholar 

  12. Burda C, Chen X, Narayanan R, El-Sayed MA (2005) Chem Rev 105:1025–1102

    Article  CAS  Google Scholar 

  13. Shankar SS, Rai A, Ahmad A, Sastry MJ (2004) Colloid Interf Sci 275:496–502

    Article  CAS  Google Scholar 

  14. Guzmán MG, Dille J, Godet S (2009) Internat J Chem Biol Eng 2:104–111

    Google Scholar 

  15. Makarov VV, Makarova SS, Love AJ, Sinitsyna OV, Dudnik AO, Yaminsky IV, Taliansky ME, Kalinina NO (2014) Langmuir 30:5982–5988

    Article  CAS  Google Scholar 

  16. Raveendran P, Fu J, Wallen SL (2003) J Am Chem Soc 125:13940–13941

    Article  CAS  Google Scholar 

  17. Goshisht MK, Moudgil L, Rani M, Khullar P, Singh G, Kumar H, Singh N, Kaur G, Bakshi MS (2014) J Phys Chem C 118:28207–28219

    Article  CAS  Google Scholar 

  18. Bakshi MS (2011) J Phys Chem C 115:13947–13960

    Article  CAS  Google Scholar 

  19. Mahal A, Goshisht MK, Khullar P, Kumar H, Singh N, Kaur G, Bakshi MS (2014) Phys Chem Chem Phys 16:14257–14270

    Article  CAS  Google Scholar 

  20. Hussain S, Al-Thabaiti SA, Khan Z (2014) Bioprocess Biosyst Eng 37:1727–1735

    Article  CAS  Google Scholar 

  21. Lee M-H, Oh S-G, Suh K-D, Kim D-G, Sohn D (2002) Colloids Surf A: Physicochem Eng Asp 210:49–60

    Article  CAS  Google Scholar 

  22. Mantion A, Guex AG, Foelske A, Mirolo L, Fromm KM, Painsi M, Taubert A (2008) Soft Matter 4:606–617

    Article  CAS  Google Scholar 

  23. Ramanathan R, O’Mullane AP, Parikh RY, Smooker PM, Bhargava SK, Bansal V (2011) Langmuir 27:714–719

    Article  CAS  Google Scholar 

  24. Bansal V, Ramanathan R, Bhargava SK (2011) Aust J Chem 64:279–293

    Article  CAS  Google Scholar 

  25. Obaid AY, Al-Thabaiti SA, El- Mossalamy EH, Al-Harbi LM, Khan Z Arabian J Chem. doi:10.1016/j.arabjc.2014.12.035

  26. AL-Thabaiti SA, Khan Z, Hussain S (2015) J Mol Liq 212:316–324

    Article  CAS  Google Scholar 

  27. Khan Z, Al-Thabaiti SA, Obaid AY, Al-Youbi AO (2011) Colloids Surf B: Biointerfaces 82:513–517

    Article  CAS  Google Scholar 

  28. Sau TK, Murphy CJ (2004) J Am Chem Soc 126:8648–8649

    Article  CAS  Google Scholar 

  29. Chen SH, Wang ZL, Ballato J, Foulger SH, Carroll DL (2003) J Am Chem Soc 125:16186–16187

    Article  CAS  Google Scholar 

  30. Kuo C-H, Huang MH (2005) Langmuir 21:2012–2016

    Article  CAS  Google Scholar 

  31. Chen J, Herricks T, Xia Y (2005) Angew Chem Int Ed 44:2589–2592

    Article  CAS  Google Scholar 

  32. Bakshi MS (2009) Langmuir 25:12697–12705

    Article  CAS  Google Scholar 

  33. Dreaden EC, Mackey MA, Huang X, Kang B, El-Sayed MA (2011) Chem Soc Rev 40:339–3404

    Article  Google Scholar 

  34. Li QQ, Wang G, Zhang M, Cuff CF, Huang L, Reed E (2009) Oncol Rep 22:161–170

    CAS  Google Scholar 

  35. Rahman MTU, Shilpi JA, Ahmed M, Hossain CF (2005) J Ethnopharmacalogy 99:203–209

    Article  Google Scholar 

  36. Kweka EJ, Nkya HM, Lyaruu L, Kimaro EE, Mwangonde BJ, Mahande AM (2009) J Cell Anim Biol 3:171–174

    CAS  Google Scholar 

  37. Khan MN, Khan TA, Khan Z, Al-Thabaiti SA (2015) Bioprocess Biosyst Eng 38:2397–2416

    Article  CAS  Google Scholar 

  38. De S, Pal A, Jana NR, Pal T (2000) J Photochem Photobiol A: Chem 131:111

    Article  CAS  Google Scholar 

  39. Al-Thabaiti SA, Al-Nowaiser FM, Obaid AY, Al-Youbi AO, Khan Z (2008) Colloids Surf B: Biointerfaces 67:230–237

    Article  CAS  Google Scholar 

  40. Mock JJ, Barbic M, Smith DR, Schultz DA, Schultz S (2002) J Chem Phys 116:6755–6759

    Article  CAS  Google Scholar 

  41. Wiley B, Sun Y, Mayers B, Xi Y (2005) Chem-Eur J 11:454–463

    Article  CAS  Google Scholar 

  42. Creighton JA, Blatchford CG, Albrecht MG (1979) J Chem Soc, Faraday Trans 75:790–798

    Article  CAS  Google Scholar 

  43. Sharma VK, Yngard RA, Yekaterina L (2009) Adv Colloid Interf Sci 145:83–96

    Article  CAS  Google Scholar 

  44. Ulkur E, Oncul O, Karagoz H, Yeniz E, Celikoz B (2005) Burns 31:874–877

    Article  Google Scholar 

  45. Panacek A, Kvıtek L, Prucek R, Kolar M, Vecerova R, Pizurova N, Sharma VK, Nevecna T, Zboril R (2006) J Phys Chem B 110:16248–16253

    Article  CAS  Google Scholar 

  46. Rai M, Yadav A, Gade A (2009) Biotechnol Adv 27:76–83

    Article  CAS  Google Scholar 

  47. Landsdown ABG (2002) J Wound Care 11:125–138

    Article  Google Scholar 

  48. Castellano JJ, Shafii SM, Ko F, Donate G, Wright TE, Mannari RJ, Payne WG, Smith DJ, Robson MC (2007) Int Wound J 4:114–122

    Article  Google Scholar 

  49. Zhao Y, Sun X, Zhang G, Trewyn BG, Slowing II, Lin VSY (2011) ACS Nano 5:1366–1375

    Article  CAS  Google Scholar 

  50. Yu T, Malugin A, Ghandehari H (2011) ACS Nano 5:5717–5728

    Article  CAS  Google Scholar 

  51. Mahal A, Khullar P, Kumar H, Kaur G, Singh N, Jelokhani-Niaraki M, Bakshi MS (2013) ACS Sustain Chem Eng 1:627–639

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Chemistry Department, Faculty of Science, King Abdulaziz University, P.O. Box 80203, Jeddah, 21589, Saudi Arabia

    Elham Shafik Aazam & Zoya Zaheer

Authors
  1. Elham Shafik Aazam
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zoya Zaheer
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zoya Zaheer.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Aazam, E.S., Zaheer, Z. Growth of Ag-nanoparticles in an aqueous solution and their antimicrobial activities against Gram positive, Gram negative bacterial strains and Candida fungus. Bioprocess Biosyst Eng 39, 575–584 (2016). https://doi.org/10.1007/s00449-016-1539-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00449-016-1539-3

Keywords

Search

Navigation