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Development of a two-step process for production of 3-hydroxypropionic acid from glycerol using Klebsiella pneumoniae and Gluconobacter oxydans


In this work, a two-step process was developed for the production of 3-hydroxypropionic acid from glycerol. In the first step, glycerol was converted to 1,3-propanediol by Klebsiella pneumonia. In the second step, the 1,3-propanediol was converted into 3-hydroxypropionic acid by Gluconobacter oxydans. In a 7.0 L bioreactor, the whole process took 54 h, consumed 480 g glycerol and produced 242 g 3-hydroxypropionic acid. The conversion rate of glycerol to 3-hydroxypropionic acid was 50.4 % (g g−1). The final concentration of 3-hydroxypropionic acid arrived 60.5 g L−1. The process was effective for 3-HP production from glycerol and it might provide a new approach to the biosynthesis of 3-HP from a cheap starting material. Moreover, in this paper, it was first reported that the by-product of 3-hydroxypropionic acid production from 1,3-propandeiol was acrylic acid.

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This study was financially supported by Natural Science Foundation of China (No. 21506057), and Natural Science Foundation of Shanghai (No. 2013ZR1412100), and was partly supported by the National major science and technology projects of China (No. 2012ZX09304009), 863 Program (No. 2012AA022201C), and the Fundamental Research Funds for the Central Universities.

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Correspondence to Dongzhi Wei.

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Zhao, L., Lin, J., Wang, H. et al. Development of a two-step process for production of 3-hydroxypropionic acid from glycerol using Klebsiella pneumoniae and Gluconobacter oxydans . Bioprocess Biosyst Eng 38, 2487–2495 (2015).

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  • 3-Hydroxypropionic acid
  • Glycerol
  • Klebsiella pneumoniae
  • Gluconobacter oxydans
  • Two-step process