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Bioprocess and Biosystems Engineering

, Volume 38, Issue 8, pp 1437–1445 | Cite as

Immobilization of Aspergillus niger lipase on chitosan-coated magnetic nanoparticles using two covalent-binding methods

  • Yolanda Osuna
  • José Sandoval
  • Hened Saade
  • Raúl G. López
  • José L. Martinez
  • Edith M. Colunga
  • Gabriela de la Cruz
  • Elda P. Segura
  • Fernando J. Arévalo
  • María A. Zon
  • Héctor Fernández
  • Anna IlyinaEmail author
Original Paper

Abstract

Aspergillus niger lipase immobilization by covalent binding on chitosan-coated magnetic nanoparticles (CMNP), obtained by one-step co-precipitation, was studied. Hydroxyl and amino groups of support were activated using glycidol and glutaraldehyde, respectively. Fourier transform infrared spectrometry, high-resolution transmission electron microscopy and thermogravimetric analysis confirmed reaction of these coupling agents with the enzyme and achievement of a successful immobilization. The derivatives showed activities of 309.5 ± 2.0 and 266.2 ± 2.8 U (g support)−1 for the CMNP treated with glutaraldehyde and with glycidol, respectively. Immobilization enhanced the enzyme stability against changes of pH and temperature, compared to free lipase. Furthermore, the kinetic parameters K m and V max were determined for the free and immobilized enzyme. K m value quantified for enzyme immobilized by means of glutaraldehyde was 1.7 times lowers than for free lipase. High storage stability during 50 days was observed in the immobilized derivatives. Finally, immobilized derivatives retained above 80 % of their initial activity after 15 hydrolytic cycles. The immobilized enzyme can be applied in various biotechnological processes involving magnetic separation.

Keywords

A. niger lipase Immobilization on magnetic nanoparticles coated with chitosan Glycidol and glutaraldehyde as coupling agents 

Notes

Acknowledgments

The work is financially supported by bilateral grant SRE-CONACYT No. 187488 (Mexico) and PICT CAMEB-2012-No. 0855 (Argentina).

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Yolanda Osuna
    • 1
  • José Sandoval
    • 2
  • Hened Saade
    • 3
  • Raúl G. López
    • 3
  • José L. Martinez
    • 1
  • Edith M. Colunga
    • 2
  • Gabriela de la Cruz
    • 2
  • Elda P. Segura
    • 1
  • Fernando J. Arévalo
    • 4
  • María A. Zon
    • 4
  • Héctor Fernández
    • 4
  • Anna Ilyina
    • 1
    Email author
  1. 1.Cuerpo Académico (CA) de NanobiocienciaUniversidad Autónoma de CoahuilaSaltilloMexico
  2. 2.CA de Química Analítica, Facultad de Ciencias QuímicasUniversidad Autónoma de CoahuilaSaltilloMexico
  3. 3.Departamento de Procesos de PolimerizaciónCentro de Investigación en Química AplicadaSaltilloMexico
  4. 4.Departamento de Química. Grupo de Electroanalítica (GEANA). Facultad de Ciencias Exactas, Físico-Químicas y NaturalesUniversidad Nacional de Río CuartoRío CuartoArgentina

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