Bioprocess and Biosystems Engineering

, Volume 38, Issue 6, pp 1065–1073 | Cite as

Industrial vitamin B12 production by Pseudomonas denitrificans using maltose syrup and corn steep liquor as the cost-effective fermentation substrates

  • Wei Xia
  • Wei Chen
  • Wei-fu Peng
  • Kun-tai LiEmail author
Original Paper


The aerobic Pseudomonas denitrificans is widely used for industrial and commercial vitamin B12 fermentation, due to its higher productivity compared to the anaerobic vitamin B12-producing microorganisms. This paper aimed to develop a cost-effective fermentation medium for industrial vitamin B12 production by P. denitrificans in 120,000-l fermenter. It was found that maltose syrup (a low-cost syrup from corn starch by means of enzymatic or acid hydrolysis) and corn steep liquor (CSL, a by-product of starch industry) were greatly applicable to vitamin B12 production by P. denitrificans. Under the optimal fermentation medium performed by response surface methodology, 198.27 ± 4.60 mg/l of vitamin B12 yield was obtained in 120,000-l fermenter, which was close to the fermentation with the refined sucrose (198.80 mg/l) and was obviously higher than that obtained under beet molasses utilization (181.75 mg/l). Therefore, maltose syrups and CSL were the efficient and economical substrates for industrial vitamin B12 fermentation by P. denitrificans.


Pseudomonas denitrificans Vitamin B12 Industrial fermentation Maltose syrup Corn steep liquor 



This work was financially supported by the National Natural Science Foundation of China (Grant No. 31460019), Jiangxi Key Project of Basic Research Program (20143ACB21005), Training Program for Young Scientists of Jiangxi Provincial Department of Science and Technology (20142BCB23025), and International Scientific and Technological Cooperation Projects of Jiangxi Provincial Department of Science and Technology (20141BDH80033). We are also grateful to Shijiazhuang Pharma Group Hua Rong Pharmaceutical Co. Ltd for supporting this work.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  1. 1.Nanchang Key Laboratory of Applied Fermentation TechnologyJiangxi Agricultural UniversityNanchangChina

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