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Bioprocess and Biosystems Engineering

, Volume 38, Issue 5, pp 989–993 | Cite as

Enhancing bio-hydrogen production from sodium formate by hyperthermophilic archaeon, Thermococcus onnurineus NA1

  • Seung Seob Bae
  • Hyun Sook Lee
  • Jung Ho Jeon
  • Jung-Hyun Lee
  • Sung Gyun KangEmail author
  • Tae Wan KimEmail author
Short Communication

Abstract

Hyperthermophilic archaeon, Thermococcus onnurineus NA1 was reported to grow on formate producing hydrogen (H2). In this study, to sustain high H2 production rate and demonstrate the feasibility of mass production of H2, high cell density cultivation of T. onnurineus NA1 on sodium formate was employed under optimized conditions. From batch cultures, it was observed that the salinity of medium, significantly changed by the addition of formate salt and pH-adjusting agent, crucially affected cell growth and H2 production. With salinity carefully controlled between 3.7 and 4.6 %, 400 mM sodium formate was found to be an optimal initial concentration for maximizing cell growth-associated H2 production. Under optimal conditions, the repeated batch culture with cell recycling showed high cell density of OD600 of 1.7 in 3 and 30 L bioreactor, and the volumetric H2 production rate was enhanced up to 235.7 mmol L−1 h−1, which is one of the highest values reported to date.

Keywords

Hyperthermophilic archaeon Thermococcus onnurineus NA1 Bio-hydrogen Sodium formate Repeated batch with cell recycling 

Notes

Acknowledgments

This work was supported by the KIOST in-house program (PE99212), and the Development of Biohydrogen Production Technology Using the Hyperthermophilic Archaea program of the Ministry of Oceans and Fisheries in the Republic of South Korea.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Seung Seob Bae
    • 1
    • 2
  • Hyun Sook Lee
    • 1
    • 2
  • Jung Ho Jeon
    • 2
    • 3
  • Jung-Hyun Lee
    • 1
    • 2
  • Sung Gyun Kang
    • 1
    • 2
    Email author
  • Tae Wan Kim
    • 1
    • 2
    Email author
  1. 1.Department of Marine BiotechnologyKorea University of Science and TechnologyDaejeonRepublic of Korea
  2. 2.Korea Institute of Ocean Science and TechnologyAnsanRepublic of Korea
  3. 3.Department of Biological SciencesMyongji UniversityYonginRepublic of Korea

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