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Bioprocess and Biosystems Engineering

, Volume 38, Issue 3, pp 457–467 | Cite as

Escherichia coli persistence kinetics in dairy manure at moderate, mesophilic, and thermophilic temperatures under aerobic and anaerobic environments

  • Pramod K. Pandey
  • Sagor Biswas
  • Venkata K. Vaddella
  • Michelle L. Soupir
Original Paper

Abstract

To assess Escherichia coli (E. coli) persistence in dairy manure, bench scale experiments were conducted under aerobic and anaerobic environments. The changes in E. coli levels in dairy manure were assessed at moderate (25 °C), mesophilic (37 °C), and thermophilic (52.5 °C) temperatures. The inactivation of E. coli at moderate, mesophilic, and thermophilic temperatures were described by linear regression equations. Subsequently, double-exponential kinetic models were developed to describe the E. coli decay curves under aerobic and anaerobic environments. The kinetics models were used to estimate E. coli log reductions at various temperatures. Results showed that the double-exponential kinetic models performed well while calculating E. coli reductions in dairy manure over the incubation period. In addition, we evaluated digestate to compare the changes in total solids and volatile solids, total organic carbon, total nitrogen, pH, and oxygen reduction potential levels in aerobic and anaerobic conditions under various temperatures. We anticipate that the results presented here will be useful for enhancing the understanding of pathogen reduction in anaerobic and aerobic processes during dairy manure treatment.

Keywords

Aerobic Anaerobic Dairy manure E. coli Inactivation 

Notes

Acknowledgments

Authors thank to Division of Agriculture and Natural Resources (ANR), University of California, Davis, and Iowa State University, Ames for supporting the work. The authors would like to thank Kendal Agee, Andrew Paxson, Charles Velasquez, and Ray Sims, students and trainees of Iowa State University, Ames, Iowa, for assistance with sample collection and analysis.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Pramod K. Pandey
    • 1
  • Sagor Biswas
    • 1
  • Venkata K. Vaddella
    • 1
  • Michelle L. Soupir
    • 2
  1. 1.Department of Population Health and Reproduction, Veterinary Medicine ExtensionUniversity of CaliforniaDavisUSA
  2. 2.Department of Agricultural and Biosystems EngineeringIowa State UniversityAmesUSA

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