Bioprocess and Biosystems Engineering

, Volume 38, Issue 2, pp 323–328 | Cite as

Enhanced acid tolerance of Rhizopus oryzae during fumaric acid production

  • Ying Liu
  • Chunwei Lv
  • Qing Xu
  • Shuang Li
  • He Huang
  • Pingkai Ouyang
Original Paper


Ensuring a suitable pH in the culture broth is a major problem in microorganism-assisted industrial fermentation of organic acids. To address this issue, we investigated the physiological changes in Rhizopus oryzae at different extracellular pH levels and attempted to solve the issue of cell shortage under low pH conditions. We compared various parameters, such as membrane fatty acids’ composition, intracellular pH, and adenosine triphosphate (ATP) concentration. It was found that the shortage of intracellular ATP might be the main reason for the low rate of fumaric acid production by R. oryzae under low pH conditions. When 1 g/l citrate was added to the culture medium at pH 3.0, the intracellular ATP concentration increased from 0.4 to 0.7 µmol/mg, and the fumaric acid titer was enhanced by 63 % compared with the control (pH 3.0 without citrate addition). The final fumaric acid concentration at pH 3.0 reached 21.9 g/l after 96 h of fermentation. This strategy is simple and feasible for industrial fumaric acid production under low pH conditions.


Rhizopus oryzae Fumaric acid Low pH fermentation Acid tolerance ATP 



This work was financially supported by the National Natural Science Foundation of China (No. 21106065), the National Basic Research Program of China (No. 2013CB733605), National Science Foundation for Distinguished Young Scholars of China (No. 21225626), and the National High Technology Research and Development Program of China (No. 2011AA02A206).


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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Ying Liu
    • 1
    • 2
  • Chunwei Lv
    • 1
  • Qing Xu
    • 1
  • Shuang Li
    • 1
  • He Huang
    • 1
  • Pingkai Ouyang
    • 1
  1. 1.State Key Laboratory of Material-oriented Chemical Engineering College of Biotechnology and Pharmaceutical EngineeringNanjing Tech UniversityNanjingChina
  2. 2.Science and Technology Development DepartmentSinopec CorporationBeijingChina

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