Bioprocess and Biosystems Engineering

, Volume 37, Issue 12, pp 2529–2537 | Cite as

Kinetics of sucrose conversion to fructo-oligosaccharides using enzyme (invertase) under free condition

  • Deepa C. Khandekar
  • Tapas Palai
  • Aman Agarwal
  • Prashant K. Bhattacharya
Original Paper


The study reports the synthesis of fructo-oligosaccharide (FOS) from sucrose using invertase derived from Saccharomyces cerevisiae. The reaction was conducted in a batch mode under free enzyme condition. Fructo-oligosaccharide formation was detected at a high sucrose concentration of over 200 g/L. The investigation was extended to study the effect of different parameters such as initial sucrose concentration (ISC), pH, and enzyme concentration. A maximum FOS yield of 10 % (dry basis) was observed using 525 g/L of ISC, with 6 U/mL of the enzyme, and pH 5.5 at 40 °C. 1-Kestose was the major product of among different forms of FOS. The FOS yield increased with an increase in sucrose concentration up to 525 g/L, beyond which it started to decrease. However, the maximum FOS yield was not affected by the increasing concentration of the enzyme beyond a certain level (2 U/mL). Furthermore, the activity of enzyme slightly increased with an increase in the pH up to 6, and thereafter it declined. Addition of glucose decreased the FOS yield because of enzyme inhibition. A five-step, ten-parameter model was developed, for which the simulation was performed in COPASI. The results predicted by the model were consistent with the experimental data.


Fructo-oligosaccharide Invertase Kinetic model Sucrose Saccharomyces cerevisiae 

List of symbols




Objective function of rate constants






Rate constants for step reactions


Michaelis–Menten constant




Unit of enzyme


Maximum reaction rate





High performance liquid chromatography


Initial sucrose concentration





One of the authors (PKB) gratefully acknowledges the Department of Biotechnology (DBT), Government of India, for partial financial support against sanction order number: BT/PR14530/PID/06/599/2010. Further, the first author (DCK) gratefully acknowledges Prof. Ashok Kumar of the Department of Biological Sciences and Bioengineering at I.I.T.-Kanpur, for useful discussions in carrying out the experimental work.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Deepa C. Khandekar
    • 1
  • Tapas Palai
    • 1
  • Aman Agarwal
    • 1
  • Prashant K. Bhattacharya
    • 1
  1. 1.Department of Chemical EngineeringIndian Institute of Technology KanpurKanpurIndia

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