Bioprocess and Biosystems Engineering

, Volume 37, Issue 11, pp 2235–2242 | Cite as

Ethanol production from sunflower meal biomass by simultaneous saccharification and fermentation (SSF) with Kluyveromyces marxianus ATCC 36907

Original Paper
First Online:
Received:
Accepted:

Abstract

The lignocellulosic materials are considered promising renewable resources for ethanol production, but improvements in the processes should be studied to reduce operating costs. Thus, the appropriate enzyme loading for cellulose saccharification is critical for process economics. This study aimed at evaluating the concentration of cellulase and β-glucosidase in the production of bioethanol by simultaneous saccharification and fermentation (SSF) of sunflower meal biomass. The sunflower biomass was pretreated with 6 % H2SO4 (w/v), at 121 °C, for 20 min, for hemicellulose removal and delignificated with 1 % NaOH. SSF was performed with Kluyveromyces marxianus ATCC 36907, at 38 °C, 150 rpm, for 72 h, with different enzyme concentrations (Cellulase Complex NS22086-10, 15 and 20 FPU/gsubstrate and β-Glucosidase NS22118, with a cellulase to β-glucosidase ratio of 1.5:1; 2:1 and 3:1). The best condition for ethanol production was cellulase 20 FPU/gsubstrate and β-glucosidase 13.3 CBU/gsubstrate, resulting in 27.88 g/L ethanol, yield of 0.47 g/g and productivity of 0.38 g/L h. Under this condition the highest enzymatic conversion of cellulose to glucose was attained (87.06 %).

Keywords

Lignocellulosic biomass Helianthus annuus Enzymatic saccharification Bioethanol Thermotolerant yeast 
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Notes

Acknowledgments

The authors acknowledge the company Caramuru SA for having provided the sunflower meal and Brazilian National Research Council (CNPq) and Itaipu Technological Park (PTI) for the master grant awarded to Danielle Camargo.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Danielle Camargo
    • 1
  • Simone D. Gomes
    • 1
  • Luciane Sene
    • 1
  1. 1.Post-Graduate Program in Agricultural Engineering, PGEAGRI, Center of Exact and Technological SciencesWestern Paraná State UniversityCascavelBrazil

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