Advertisement

Bioprocess and Biosystems Engineering

, Volume 37, Issue 8, pp 1679–1684 | Cite as

Optimization of pyrene degradation by white-rot fungus Pleurotus pulmonarius F043 and characterization of its metabolites

  • Tony HadibarataEmail author
  • Zee Chuang Teh
Original Paper

Abstract

Pleurotus pulmonarius F043, a fungus collected from tropical rain forest, was used to degrade pyrene, a four-rings polycyclic aromatic hydrocarbons (PAHs), in a mineral medium broth. A maximum degradation rate of pyrene (90 %) was occurred at pH 3 and the lowest degradation rate was found in the culture at pH 10 (2 %). More than 90 % pyrene degradation was achieved at pH ranged from 3 to 5, whereas the degradation rate significantly declined when the pH was >5. The degradation of pyrene increased from 2 to 96 % when the temperature rose from 4 to 25 °C. When the temperature was increased to 60 °C resulting the lowest degradation rate into 7 %. Among the agitation rates tested, 120 rpm was the best with 95 % degradation, followed by 100 rpm (90 %). The optimum agitation range for pyrene degradation by P. pulmonarius F043 was 100–120 rpm. Among all the concentrations tested, 0.5 % Tween 80 was the best with 98 % degradation, followed by 1 % Tween 80 (90 %). The optimum concentration of Tween 80 for pyrene degradation by P. pulmonarius F043 was 0.5–1 %. The degradation rate decreased, while the concentration of Tween 80 was increased. The metabolic product was found during degradation process through the identification of gentisic acid by TLC, UV-Spectrophotometer, and GC–MS.

Keywords

Optimization Ligninolytic enzymes Pleurotus pulmonarius F043 Pyrene Gentisic acid 

Notes

Acknowledgments

This project was partly financially supported by Universiti Teknologi Malaysia (02H65) and Ministry of High Education, Malaysia (4L053).

References

  1. 1.
    Zakaria MP, Takada H, Tsutsumi S, Ohno K, Yamada J, Kouno E, Kumata H (2002) Distribution of polycyclic aromatic hydrocarbons (PAHs) in rivers and estuaries in Malaysia: a widespread input of petrogenic PAHs. Environ Sci Technol 36:1907–1918CrossRefGoogle Scholar
  2. 2.
    Sartoros C, Yerushalmi L, Beron P, Guiot SR (2005) Effects of surfactant and temperature on biotransformation kinetics of anthracene and pyrene. Chemosphere 61:1042–1050CrossRefGoogle Scholar
  3. 3.
    Sudipt KS, Om VS, Rakesh KJ (2002) Polycyclic aromatic hydrocarbons: environmental pollution and bioremediation. Trends Biotechnol 20:243–248CrossRefGoogle Scholar
  4. 4.
    Jin D, Jiang X, Jing X, Ou Z (2007) Effects of concentration, head group, and structure of surfactants on the degradation of phenanthrene. J Hazard Mat 144:215–221CrossRefGoogle Scholar
  5. 5.
    Heitkamp MA, Cerniglia CE (1988) Mineralization of polycyclic aromatic hydrocarbons by a bacterium isolated from sediment below an oil field. Appl Environ Microbiol 54:1612–1614Google Scholar
  6. 6.
    Bautista LF, Sanz R, Molina MC, Gonzalez N, Sanchez D (2009) Effect of different non-ionic surfactants on the biodegradation of PAHs by diverse aerobic bacteria. Int Biodeter Biodegr 63:913–922CrossRefGoogle Scholar
  7. 7.
    Zhu L, Feng S (2003) Synergistic solubilization of polycyclic aromatic hydrocarbons by mixed anionic–nonionic surfactants. Chemosphere 53:459–467CrossRefGoogle Scholar
  8. 8.
    Venkata Mohan S, Kisa T, Ohkuma T, Kanaly RA, Shimizu Y (2006) Bioremediation technologies for treatment of PAH-contaminated soil and strategies to enhance process efficiency. Rev Environ Sci Biotechnol 5:347–374CrossRefGoogle Scholar
  9. 9.
    Perelo LW (2010) Review: in situ and bioremediation of organic pollutants in aquatic sediments. J Hazard Mat 177:81–89CrossRefGoogle Scholar
  10. 10.
    Franzetti A, Gennaro DP, Bestetti G, Lasagni M, Pitea D, Collina E (2008) Selection of surfactants for enhancing diesel hydrocarbons-contaminated media bioremediation. J Hazard Mat 152:1309–1316CrossRefGoogle Scholar
  11. 11.
    Vidali M (2001) Bioremediation. An overview. Pure Appl Chem 73:1163–1172CrossRefGoogle Scholar
  12. 12.
    Hammel KE (1997) In: Cadisch G, Giller KE (eds) Driven by nature: plant litter quality and decomposition. CAB International, WallingfordGoogle Scholar
  13. 13.
    Bezalel L, Hadar Y, Cerniglia CE (1997) Enzymatic mechanisms involved in phenanthrene degradation by white-rot fungus Pleurotus ostreatus. App Environ Microbiol 63:2495–2501Google Scholar
  14. 14.
    Kariminiaae-Hamedaani HR, Sakurai A, Sakakibara M (2007) Decolorization of synthetic dyes by a new manganese peroxidase-producing white rot fungus. Dyes Pigm 72:157–162CrossRefGoogle Scholar
  15. 15.
    Novotny C, Erbanova P, Cajthaml T, Rothschild N, Dosoretz C, Sasek V (2000) Irpex lacteus, a white rot fungus applicable to water and soil bioremediation. Appl Microbiol Biotechnol 54:850–853CrossRefGoogle Scholar
  16. 16.
    Pointing SB (2001) Feasibility of bioremediation by white-rot fungi. Appl Microbiol Biotechnol 57:20–33CrossRefGoogle Scholar
  17. 17.
    Hadibarata T, Kristanti RA (2013) Biodegradation and metabolite transformation of pyrene by basidiomycetes fungal isolate Armillaria sp. F022. Bioproc Biosys Eng 36:461–468CrossRefGoogle Scholar
  18. 18.
    Eriksson M, Ka JO, Mohn WW (2001) Effects of low temperature and freeze-thaw cycles on hydrocarbon biodegradation in Arctic Tundra soil. Appl Environ Microbiol 67:5107–5112CrossRefGoogle Scholar
  19. 19.
    Leys MN, Bastiaens L, Verstraete W, Springael D (2004) Influence of the carbon/nitrogen/phosphorus ratio on polycyclic aromatic hydrocarbons degradation by Mycobacterium and Sphingomonas in soil. Appl Microbiol Biotechnol 66:726–736CrossRefGoogle Scholar
  20. 20.
    Mohn W, Stewart RG (2000) Limiting factors for hydrocarbon biodegradation at low temperatures in Artic soils. Soil Biol Biochem 32:1161–1172CrossRefGoogle Scholar
  21. 21.
    Hadibarata T, Yusoff ARM, Aris A, Salmiati, Hidayat T, Kristanti RA (2012) Decolorization of azo, triphenylmethane and anthraquinone dyes by laccase of a newly isolated Armillaria sp. F022. Water Air Soil Poll 223:1045–1054CrossRefGoogle Scholar
  22. 22.
    Zouari-Mechichi H, Mechichi T, Dhoui A, Sayad S, Martínez AT, Martinez MJ (2006) Laccase purification and characterization from Trametes trogii isolated in Tunisia: decolorization of textile dyes by the purified enzyme. Enzyme Microbial Technol 39:141–148CrossRefGoogle Scholar
  23. 23.
    Lejune R, Baron GV (1995) Effect of agitation on growth and enzyme production of Trichoderma reesei in bath fermentation. Appl Microbiol Biotechnol 43:249–258CrossRefGoogle Scholar
  24. 24.
    Amanullah A, Justen P, Davies A, Paul GC, Nienow AW, Thomas CR (2000) Agitation induced mycelial fragmentation of Aspergillus oryzae and Penicillium chrysogenum. Biochem Eng J 5:109–114CrossRefGoogle Scholar
  25. 25.
    Li ZJ, Shukla V, Fordyce AP, Pedersen AG, Wenger KS, Marten MR (2000) Fungal morphology and fragmentation behavior in fed-batch Aspergillus oryzae fermentation at the production scale. Biotechnol Bioeng 70:300–312CrossRefGoogle Scholar
  26. 26.
    Hadibarata T, Kristanti RA (2013) Effect of surfactants and identification of metabolites on the biodegradation of fluoranthene by basidiomycetes fungal isolate Armillaria sp. F022. Bioproc Biosys Eng. doi: 10.1007/s00449-013-1025-0
  27. 27.
    Brownawell BJ, Chen H, Zhang W, Westall JC (1997) Sorption of non-ionic surfactants on sediment materials. Environ Sci Technol 31:1735–1741CrossRefGoogle Scholar
  28. 28.
    Fytianos K, Voudrias E, Papamichali A (1998) Behavior and fate of linear alkylbenzene sulfonate in different soils. Chemosphere 36:2741–2746CrossRefGoogle Scholar
  29. 29.
    Hadibarata T, Kristanti RA (2012) Identification of metabolites from benzo[a]pyrene oxidation by ligninolytic enzymes of Polyporus sp. S133. J Environ Manag 111:115–119CrossRefGoogle Scholar
  30. 30.
    Hadibarata T, Yusoff ARM, Aris A, Kristanti RA (2012) Identification of naphthalene metabolism by white rot fungus Armillaria sp. F022. J Environ Sci 24:728–732CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  1. 1.Institute of Environmental and Water Resource Management, Faculty of Civil EngineeringUniversiti Teknologi MalaysiaSkudaiMalaysia

Personalised recommendations