Assessment of different carbohydrates as exogenous carbon source in cultivation of cyanobacteria
- 661 Downloads
Glucose is the substrate most widely used as exogenous carbon source for heterotrophic cultivation of cyanobacteria. Due to limited information about the use of different carbohydrates as carbon sources to support cyanobacterial heterotrophic metabolism, the objective of this work was to evaluate different monosaccharides (arabinose, fructose, galactose, glucose, mannose and xylose), disaccharides (lactose, maltose, sucrose and trehalose) and polysaccharides (carboxymethylcellulose, cassava starch, Hi-maize®, maltodextrin Corn Globe 1805® and xylan) as exogenous carbon source for heterotrophic culture of cyanobacterium Phormidium sp. The batch cultivation using fructose as organic carbon source resulted in the highest (p < 0.05) cell biomass (5,540 mg/L) in parallel with the highest (p < 0.05) substrate yield coefficient (0.67 mgbiomass/mgfructose). Mannose was the carbon source with the highest (p < 0.05) substrate consumption rate (3,185.7 mg/L/day) and maltodextrin was the carbohydrate with major potential to produce biomass (1,072.8 mgbiomass/L/day) and lipids (160.8 mglipids/L/day). Qualitatively, the fatty acid profiles of the lipid extract from Phormidium sp. showed predominance of saturated chains for the cultures grown with most of the carbon sources, with the exception of the ones grown with xylose and maltodextrin.
KeywordsMicroalgae/cyanobacteria Heterotrophic metabolism Carbohydrates
Funding for this research was provided by São Paulo Research Foundation (FAPESP, Brazil).
- 3.Smith AJ (1982) In: Carr NG, Whitton BA (eds) Modes of cyanobacterial carbon metabolism. The biology of cyanobacteria. University of California Press, BerkeleyGoogle Scholar
- 4.Fay P (1983) The Blue–Greens (Cyanophyta–Cyanobacteria). In: Edward Arnold (ed) The Institute of Biology’s, Studies in Biology, no 160Google Scholar
- 6.Lee YK (2004) Algal nutrition. Heterotrophic carbon nutrition. In: Richmond A (ed) Handbook of microalgal culture. Biotechnology and applied phycology. Blackwell Publishing, OxfordGoogle Scholar
- 10.Guiry MD, Guiry, GM (2013) AlgaeBase. World-wide electronic publication, National University of Ireland, Galway. http://www.algaebase.org searched on October 2013
- 12.APHA, AWWA, WEF (2005) Standard methods for the examination of water and wastewater, 21st edn. Prot City Press, BaltimoreGoogle Scholar
- 14.Hartman L, Lago RCA (1976) A rapid determination of fatty acid methyl esters from lipids. Lab Prat 22:475–476Google Scholar
- 15.StatSoft, Inc. (2004) STATISTICA for Windows (Computer program manual). http://www.statsoftinc.com
- 19.Raboy B, Padan E (1978) Active transport of glucose and α-methylglucoside in the cyanobacterium Plectonema boryanum. J Biol Chem 253:3287–3291Google Scholar
- 22.Fuchs B, Suttner P, Sterner S, Wastlhuber R, Loos E (1994) Disproportionating transglycosylase (d-enzyme) in green algae and cyanobacteria. Partial purification and characterization. Zeitschrift für Naturforschung 49:163–170Google Scholar
- 25.Wood T (1885) The pentose phosphate pathway. Academic Press Inc, OrlandoGoogle Scholar
- 28.van den Hoek C, Mann DG, Jahns HM (1995) Algae—an introduction to phycology. Press Sindicate of the University of Cambridge, New YorkGoogle Scholar