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Bioprocess and Biosystems Engineering

, Volume 37, Issue 2, pp 261–267 | Cite as

Streptomyces sp. LK3 mediated synthesis of silver nanoparticles and its biomedical application

  • L. Karthik
  • Gaurav Kumar
  • A. Vishnu Kirthi
  • A. A. Rahuman
  • K. V. Bhaskara Rao
Original Paper

Abstract

In the present study, the marine actinobacteria mediated biosynthesis of silver nanoparticles (AgNps) was achieved using Streptomyces sp LK3. The synthesized AgNps showed the characteristic absorption spectra in UV–vis at 420 nm, which confirmed the presence of nanoparticles. XRD analysis showed intense peaks at 2θ values of 27.51°, 31.87°, 45.57°, 56.56°, 66.26°, and 75.25° corresponding to (210), (113), (124), (240), (226), and (300) Bragg’s reflection based on the fcc structure of AgNps. The FTIR spectra exhibited prominent peaks at 3,417 cm−1 (OH stretching due to alcoholic group) and 1,578 cm−1 (C=C ring stretching). TEM micrograph showed that the synthesized AgNps were spherical in shape with an average size of 5 nm. Surface morphology and topographical structure of the synthesized AgNps were dignified by AFM. The synthesized AgNps showed significant acaricidal activity against Rhipicephalus microplus and Haemaphysalis bispinosa with LC50 values of 16.10 and 16.45 mg/L, respectively. Our results clearly indicate that AgNps could provide a safer alternative to conventional acaricidal agents in the form of a topical antiparasitic formulation. The present study aimed to develop a novel, cost-effective, eco-friendly actinobacteria mediated synthesis of AgNps and its antiparasitic activity.

Keywords

AFM Silver nanoparticles Streptomyces sp LK3 TEM XRD 

Notes

Acknowledgments

The authors wish to thank the Management of VIT University for providing necessary facilities to carry out this study.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • L. Karthik
    • 1
  • Gaurav Kumar
    • 1
  • A. Vishnu Kirthi
    • 2
  • A. A. Rahuman
    • 2
  • K. V. Bhaskara Rao
    • 1
  1. 1.Environmental Biotechnology Division, School of Bio Sciences and TechnologyVIT UniversityVelloreIndia
  2. 2.Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of ZoologyC. Abdul Hakeem CollegeVelloreIndia

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